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Quick propagating method of blumea riparia(Bl.)DC medicine material

A kind of cinnamon cinnamon, fast technology, applied in the field of rapid propagation and cultivation of cinnamon cinnamon, can solve the problems of slow propagation speed, genetic variation, low survival rate, etc., achieve no variation, simple operation method, transplantation The effect of high planting survival rate

Inactive Publication Date: 2007-09-26
广西桂西制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After years of mining, the resources of wild medicinal materials have been gradually reduced, and the quality of medicinal materials is unstable, which cannot meet the expanded production needs. Large-scale artificial cultivation is required as a source of raw materials for medicinal materials
At present, wild osmanthus osmanthus is mainly propagated by seeds. Due to the poor development of the seed embryo tissue of this medicinal material and the influence of factors such as seasons and growth environments, the reproductive rate of the seeds is low, and the germination rate is lower than 5%, which cannot meet the needs of large populations. In addition, due to sexual hybridization, genetic variation will occur in the offspring, resulting in unstable quality, which is not conducive to the quality control of the pharmaceutical production process
Breeding by seed propagation method, the propagation speed is slow, the survival rate is low, and it is difficult to meet the requirements of large-scale planting, so there is an urgent need for a high propagation rate and fast propagation method for seedling production
[0004] After searching, so far, there are no domestic reports on the breeding and cultivation of Ainaxiang yunnanensis, and there are no relevant research records abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] ①Induction of callus: Insert different parts of the sterilized explants into the induction medium. The explants cut out from the shoot tips are about 2cm long, the young stems are about 3.5cm long, and the old stems are about 3.5cm long , leaf size is 1.0×0.6cm 2 , The length of the bud is about 1.0cm. The number of explants to inoculate depends on different parts of the explants. Generally, the tip is 3 per bottle, the young stem is 4 segments / bottle, the old stem is 4 segments / bottle, the leaves are 10 pieces / bottle, and the flower buds are 10 pieces. / bottle. The formula of induction culture medium is MS+BA1.0mg / L+NAA0.1mg / L+sucrose 2.0%+agar powder 0.5%, (wherein MS culture medium is general basic culture medium, and the present invention selects the phytohormone market also available for sale, 2.4-D and NAA are produced by China National Pharmaceutical (Group) Shanghai Chemical Reagent Company; 6-BA, KT and IBA are produced by Sanland International Inc.; IAA is p...

Embodiment 2

[0042] ①Induction of sterile seedlings: inoculate the sterilized stems of Ainaxiang yunnanensis on the induction medium, the induction medium formula is MS+BA1.0mg / L+NAA0.1mg / L+sucrose 2.5%+agar Powder 0.5%, culture conditions are: temperature is 25-28°C, pH value is 5.8, light is 1000-1200Lux, light time is 10-12h / d, after 30 days, small buds are differentiated from the axillary buds of the stem.

[0043] ② Subculture of test-tube seedlings: When the test-tube seedlings of Diangui Ainaxiang grow to 1.5cm, cut the buds and transfer them to MS+BA1.0mg / L+NAA0.2mg / L+Met1.0mg / L+sucrose 2.5 % + agar powder 0.5% on the proliferation medium, the culture conditions are the same as before, 20 days after inoculation, clusters of buds will gradually sprout from the base of the young shoots, and each young shoot can differentiate about 7 to 8 plants on average, and then the young shoots are cut off and transformed into Into the same medium for repeated culture, a large number of multiplic...

Embodiment 3

[0048] ①Selection of strong branches: The branches of Diangui Ainaxiang collected from the wild are selected with a diameter of 0.25-0.5cm, and cut into small sections of 15-40cm.

[0049] ②Treatment before cutting: Wash the cut branches and soak them in a solution composed of NAA, IAA, and IBA for 5 hours. The concentrations of NAA, IAA, and IBA in the solution are 0.5-2.5 mg / L.

[0050] ③Cuttage seedling raising: insert the treated branches in the sand pond, cultivate them in shade and moisture, and sprouts will grow at the base of the petioles of the branches in about 2 weeks, and continue to cultivate for about 4 weeks to grow new roots. The transplanting survival rate reaches 90%. above.

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PUM

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Abstract

The invention provides a method for quickly cultivating Blumea riparia medicinal material, which comprises that collecting Blumea riparia explants in wild, which it contains stem sharp, young stem, old stem, blades, bulbs, and seeds, to be washed and disinfected, and using plant organism quick cultivating method and grafting cultivating method to quickly cultivate the Blumea riparia, cultivating seeds, while the obtained seed can be directly used in field. The invention has high cultivate speed, short period as 2-3 months, with high survival rate more than 90%, simple operation, simple device, low cost, and application in whole year. And the product cultivated by the invention has stable gene character, without variation.

Description

technical field [0001] The invention relates to a plant regeneration method through tissue culture technology, in particular to a method specially used for the rapid propagation and cultivation of Ainaxiang yunnanensis. Background technique [0002] Diangui Ainaxiang has been identified by experts as Bloumea megacephala (Randeria) Chang et Tseng, a plant of the genus Ainaracea of ​​Compositae, also known as Huaainaxiang and Guanya. It is the main product of Guangxi Guixi Pharmaceutical Co., Ltd. The main raw material, the whole herb is used as medicine. The product has the functions of promoting blood circulation, removing blood stasis, hemostasis and regulating menstruation. It is used to treat blood stagnation, menorrhagia, long menstrual period, postpartum lochia and other gynecological blood disorders. The total effective rate is 96%. Above, the annual sales income of the product exceeds 60 million yuan, which is a plant with extremely high economic value. [0003] The ...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 包国庆韦鹏霄岑秀芬陈卓张明珍
Owner 广西桂西制药有限公司
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