The invention provides a production method of minitype potato seeds bred by directly using potato stem segments, relating to the minitype potato seed high power propagation technology. In the invention, potato detoxification test tube tissue culture plantlet stem segments are used as a raw material, and then are directly implanted in an MS liquid or solid induction medium without any plant growth hormone, and the minitype potato seeds capable of sprouting are induced and produced in 6-8 weeks. The tissue culture plantlet stem segments are directly bred without changing the medium, thus obtaining the potato seeds in one step. The method has the advantages of saving time, labor and cost, having simple equipment, less used plantlets, less consumed time, rapid propagation and high efficiency and the like. The breeding period is 1.5-2 months, production can be performed for 5-6 periods each year, and the breeding chamber at unit area can produce 500,000-600,000 minitype potato seeds, thus making full use of the tissue culture chamber production space, greatly improving the output of the detoxification minitype potato seeds at each unit area, and increasing the production efficiency at each unit area by 50-100 times. The method is suitable for large-scale factory production of minitype potato seeds.