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Japanese maple air layering propagation method
The invention discloses a Japanese maple air layering propagation method which includes selecting a seed tree, selecting branches, air layering, forming new trees, and transplanting new trees. The method has the advantages that the branches, grown 2-3 years, on a growing Japanese maple are used for air layering, allowing the branches to grow on the seed tree in a nutritious manner for 65-72 days, and forming a root system at the ringing cut; the branch rooting rate is 80%, and the transplanting survival rate can reach more than 78%; the air layering propagation method can keep the excellent hereditary features of the seed Japanese maple; the method is simple in technology, convenient to operate, low in cost, high in survival rate and important for Japanese maple seedling fast propagation.
Owner:SICHUAN COLORLINK CO LTD
Method using tissue-subculture sprouts of oil-tea camellia to raise seedlings by grafting
InactiveCN101822160ATo meet the needs of excellent clones, high-quality and high-yielding Camellia oleifera seedlingsGood genetic traitsSeed and root treatmentClimate change adaptationCamellia oleiferaBiology
The invention discloses a method using tissue-subculture sprouts of oil-tea camellia (Camellia oleifera Abel) to raise seedlings by grafting, particularly a method using the tissue-subculture sprout of high-grade oil-tea camellia as a scion and using the germinated germ of oil-tea camellia seeds as a stock to culture a novel plant in a shed sealed by plastic film at 10 to 35 DEG C with the air humidity being 100% on the basis of the method of cleft grafting or slope grafting, wherein the stock seeds are stored at 4 to 5 DEG C by humid sand in an icehouse having high grafting capacity and seed storage capacity; and the grafting process is carried out day by day, and the germination acceleration is carried out group by group, accordingly, the survival rate reaches 75% to 95%. The method of the invention has the advantages of high reproductive rate, high seedling raising capacity, simple method, easy operation and low material consumption; and meanwhile, the offspring can maintain the good hereditary property of the stock plant, thus solving the problem that a large amount of improved branches and budlings are required for the existing method for the vegetative propagation of oil-tea camellia, such as cuttage propagation and seedling-raising by germ-stock grafting, and meeting the requirements of high-grade and high-quality clonal oil-tea camellia sprouts for the development of the oil-tea camellia industry in China.
Owner:GUANGXI FORESTRY RES INST
Cuttage rapid-propagation seedling culture method for elevated sand bed camphora wood cutting orchard tissue culture seedling twigs
ActiveCN102301883ALow costGood genetic traitsGreenhouse cultivationCultivating equipmentsSocial benefitsPlantlet
The invention discloses a cuttage rapid-propagation seedling culture method for elevated sand bed camphora wood cutting orchard tissue culture seedling twigs. The method comprises the following steps: taking semi-lignified twigs formed by camphora wood cutting orchard tissue culture seedling planted on the elevated sand bed as a cutting wood; treating the cutting wood by greenplant growth regulator-ABT6 rooting solution; inserting the treated cutting wood into a mixed medium which contains peat soil and coconut bran in a weight ratio of 3:(1-1.5); culturing on a pressure lifting seedling culture bed capable of automatically controlling temperature, controlling moisture and controlling light; controlling parameters of air humidity to 85%, medium moisture content to 50% and illumination intensity to 1500lux; and after one month, causing the cutting wood to form an integral plant, wherein the cuttage survival rate is 80-85%. The method has the advantages of scientific seedling culture facility, high propagation speed, large seedling culture amount, short seedling culture time, high afforestation survival rate, low wood culture cost, good economic benefit and social benefit and the like, offspring can keep the excellent hereditary character of a stock plant, and the asexual seedling culture of camphora fine variety is achieved.
Owner:GUANGXI FORESTRY RES INST
Ecological-floating-bed-based water body ecological restoration method
InactiveCN105645594AExtended service lifeHigh strengthWater treatment parameter controlEnergy based wastewater treatmentFiberEcological environment
The invention relates to an ecological-floating-bed-based water body ecological restoration method which comprises the following steps: 1) establishing an ecological floating bed, adding biological activated carbon into each growing basket, and suspending rope-shaped carbon fiber ecological grass on the bottom of the floating bed; 2) growing emerging plants in a first aquatic plant growing region, wherein the emerging plants are composed of more than two of Canna generalis, Thalia dealbata, Iris wilsonii, calamus, purple loosestrife, bamboo reed, reed, cyperus altrnlifolius and Pontederia corda; growing a floating leaf plant or submerged plant in a second aquatic plant growing region, wherein the floating leaf plant is Ludwigia stipulacea, and the submerged plant is watermilfoil; and 3) reaping: carrying out periodical reaping according to the growth condition of the aquatic plants. The multiple ornamental plants and plant species with higher synergic decontamination capacity are reasonably blended and grown to establish a stable floating bed phytocommunity, thereby enhancing the synergistic actions among organisms, enhancing the restorability of the ecological floating bed for the water ecological environment, effectively enhancing the purifying effect of the ecological floating bed, and having stabler purifying effects.
Owner:广东景泽生态环境股份有限公司
Erythrophloeum fordii cutting propagation method
ActiveCN103460931ANarrow growth spaceResistant to lignificationHorticultureDiseaseErythrophloeum fordii
The invention discloses an erythrophloeum fordii cutting propagation method which includes the procedures of stock plant selection, cutting orchard building, stock plant management, cutting slip picking, cutting and post-cutting management. Excellent robust nursery plants free of pest diseases are selected as stock plants and are densely arranged in a rectangular piece to build a cutting orchard, top cutting is conducted to promote sprouting, twigs or semi wood is selected as cutting scions which are inserted in container cups filled with substrates, and cutting seedlings are cultured to obtain excellent robust erythrophloeum fordii cutting seedlings. The method solves the problem that erythrophloeum fordii is difficult in vegetative propagation, has the advantages of being free of geographic limitation, high in propagation speed, simple, easy to operate, high in survival rate and the like, maintains good hereditary character of the erythrophloeum fordii, has great significance on protection and expansion of propagation and planting of the erythrophloeum fordii and has good economical benefit, social benefit and ecological benefit.
Owner:GUANGXI FORESTRY RES INST
Acid protease and preparation method thereof
InactiveCN101638647AHigh enzyme productionImprove performanceHydrolasesPeptide/protein ingredientsMicroorganismYeast
The invention provides acid protease which is derived from microorganism sources and a preparation method thereof. The acid protease is characterized in that a. enzymology characteristics: the optimumpH is 2.5-3.5, the stable pH is 2.5-6.0, the optimum temperature is 40-50 DEG C, and the temperature stability ranges from 30 DEG C to 50 DEG C; b. with A. niger as an enzyme producing strain and wheat bran as main materials, the acid protease is prepared by solid fermentation process; the fermenting and enzyme producing capabilities are more than or equal to 47000u / g (based on dry yeast), the yield of liquid enzyme is more than or equal to 85% and that of solid enzyme is more than or equal to 80%, which all reach the food grade hygienic standards. The acid protease takes grain processing by-products as main raw materials and has high fermentation enzyme activity, high enzyme extraction purification yield and low production cost. The acid protease is suitable for serving as the preparation raw material or the additive of the products in such industries as tanning, medicine, brewing and feed.
Owner:SHANDONG LONGKETE ENZYME PREPARATION
Tissue culture rapid propagation method of eucalyptus cloeziana
ActiveCN105454047AReliable and reliableCultivation goals are clearClimate change adaptationAfforestationBudCell budding
The invention discloses a tissue culture rapid propagation method of eucalyptus cloeziana. The method adopts an excellent single plant of an excellent eucalyptus cloeziana family, which is cut down and promoted to germinate, a bud serves as an explant, a stem section with a resting bud induces a sterile bud in vitro, the sterile bud is obtained without a callus seedling path, the stem section of the explant directly grows a side bud or an adventitious bud, and the sterile bud is subjected to subculture enrichment culture, rooting culture and rooting seedling transplanting to obtain a regenerated plant. The tissue culture rapid propagation method of eucalyptus cloeziana is lower in operation difficulty, stable in the growth of a sterile bud propagation system and good in repeatability and can be used for scale expanding propagation repeatedly, after the rooting culture of cut buds, the rooting rate is higher, the popularization value is realized, rooting seedlings can survive after transplanting, seedlings normally grow, the obtained regenerated plant can robustly grow in a large field, a survival rate of afforestation is more than 95% and excellent inheritable characters of the selected plant can be kept.
Owner:广西壮族自治区国有东门林场
Method for asexually and rapidly propagating homalium hainanense
InactiveCN103749129ASolve the problem of the shortage of a large number of well-developed varietiesAccelerate the development of afforestationHorticultureDiseaseShoot
The invention discloses a method for asexually and rapidly propagating homalium hainanense. The method includes working procedures for collecting seeds, constructing cutting orchards, managing mother plants, collecting shoots for cutting, performing cutting and managing cutting seedlings, and particularly includes selecting excellent and healthy mature trees without disease and pest injury to be used as mother trees for seed collection; collecting the seeds and conventionally accelerating germination of the seeds; transplanting sprouts of the seeds on seedling growing beds to culture seedlings and using the seedling growing beds as the cutting orchards; pruning tops of the seedlings to promote germination; cultivating totally-lignified branches to be used as the cutting scions; performing cutting on the scions in container cups filled with media; cultivating cutting seedlings to acquire the excellent and healthy cutting seedlings of the homalium hainanense. The method for asexually and rapidly propagating the homalium hainanense has the advantages that the homalium hainanense can be asexually propagated by means of cutting, so that the seedlings keep original excellent genetic characters of female parents, large quantities of seedlings can be neatly outputted from the orchards, the problem of shortage in improved varieties due to massive development of the homalium hainanense can be solved, afforestation development of the homalium hainanense can be accelerated, living requirements of people on furniture made of precious wood can be met, and the method has an important significance.
Owner:GUANGXI FORESTRY RES INST
Planting method of high-yield pueraria lobata
InactiveCN108157101AEasy to grow seedlingsIncrease planting yieldBio-organic fraction processingExcrement fertilisersSporeLobata
The invention relates to the technical field of pueraria lobata planting, in particular to a planting method of high-yield pueraria lobata. The method comprises the following steps that during planting, front surfaces and reverse surfaces of pueraria lobata seedlings are distinguished, the smooth surfaces face upwards, and the surfaces with spores face downwards and are in contact with soil; during planting, the pueraria lobata seedlings are planted at the angle of 45 degrees, when many pueraria lobata seedlings grow, small and bad pueraria lobata seedlings need to be cut off, 1-3 good-qualityroots are reserved, and all the seedlings are evenly placed in pits separately; during planting, the head directions of the pueraria lobata seedlings are unified; during planting, soil covers the spores, and pueraria lobata heads are exposed; during planting, fine and loose soil is taken by hands to cover the pueraria lobata seedlings, then the soil is slightly pressed by hands, and then the pitsare filled with a large amount of soil; after planting, each plant is watered with 1-3 kg of rooting water immediately, and better growth and development of the pueraria lobata seedlings are guaranteed. According to the planting method, a traditional planting method of pueraria lobata is improved, the yield of pueraria lobata is increased, and the method is beneficial to planting and popularization of pueraria lobata.
Owner:镇宁自治县虎头园林绿化有限公司
Jackfruit branch topping and seedling growing method
InactiveCN104012355AGood genetic traitsSimple technologyBiocidePlant growth regulatorsDiseaseEthephon
The invention discloses a jackfruit branch topping and seedling growing method. Firstly, robust branches without diseases and pests are chosen from a jackfruit seed tree with excellent quality; secondly, girdling is conducted on the portion 15-20 cm away from the position where the branches are divaricated, 0.01%-0.06% ABT-1 solution is smeared on a girdling opening by one circle, the girdling position is bound with a wet matrix and wrapped in a thin film, and the two ends of the girdling position are bound through ropes; after branch topping is conducted for 5-25 days, diluted ethephon solution is added to a thin film bag; after branch topping is conducted for 40-50 days, a root system gradually grows from the girdling opening, and half-cutting treatment is conducted on the lower ends of the branches bound by the ropes; after half-cutting treatment is conducted for 20-30 days, the branches are cut off from the half-cut positions and subjected to heeling and transplanting. The jackfruit branch topping and seedling growing method is simple, operation is easy, the seedling growing period is short, the rooting percentage is high, the survival rate of transplanting is high, limitation of seasons is avoided, and the quality of grown seedlings is guaranteed at the same time.
Owner:SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI
Method for grafting and propagating oriental cherry by use of cherry rootstock
The invention relates to a method for grafting and propagating oriental cherry by use of cherry rootstock. The method comprises the following steps: selecting the current-year cherry rootstock as a rootstock, grafting, cutting and managing, and applying dedicated fertilizers in rootstock cultivating and cutting stages respectively. The affinity of the rootstock is strong, the cutting seedling root system is flourishing, the budding survival rate is high, the grafting survival rate is more than or equal to 98%, the cutting survival rate is more than or equal to 99%, and the transplanting survival rate is more than or equal to 98%. The rootage is easy by cuttage and the root system is large and is increased by 40% or more compared with the root system of a common grafted seedling.
Owner:烟台市林业技术推广站
Ultra-low-temperature Pleurotus eryngii spawn preservation method for factory production
An ultra-low-temperature Pleurotus eryngii spawn preservation method for factory production includes 1, preserved spawn preparation; 2, protective agent preparation; and 3, preserving operation. The step of preserving operation includes (1), slicing agar of mother spawn source with mycelia into the bean sizes through an inoculating needle by sterile operation, placing the 4 to 6 agar slices into one sterile test tube, then adding the protective agent till covering the agar slices, sealing the sterile test tube, and marking through mark pens; (2), punching holes at the bottom, cover and side wall of plastic bottles, placing the sterile test tubes to the plastic bottles, then placing the plastic bottles the a ultra-low-temperature freezer, and decreasing the temperature to -80 to -150 DEG C at the speed of decreasing 1+-0.1 DEG C in every minute. By the aid of the ultra-low-temperature Pleurotus eryngii spawn preservation method for factory production, the Pleurotus eryngii spawn can be maintained in extreme slow metabolism state for 3 to 5 years generally, and excellent genetic characteristics can be maintained constantly.
Owner:HUNAN AGRICULTURAL UNIV
Method for establishing human amniotic fluid stem cell line
InactiveCN101928694ARapid separation methodEffective separation methodMicroorganism based processesTissue cultureAmniotic fluidStem cell line
The invention relates to a method for establishing a human amniotic fluid stem cell line. The method comprises the following steps of: 1) performing isolated culture on primary amniotic fluid stem cells; 2) separating and purifying the primary amniotic fluid stem cells subjected to isolated culture; and 3) performing monoclonal screening and amplification culture on the separated amniotic fluid stem cells so as to establish the amniotic fluid stem cell line. The invention provides a rapid, effective and safe method for establishing the human amniotic fluid stem cell line, can realize long-term amplification of the human amniotic fluid stem cells in vitro, can provide a novel seed cell source for tissue engineering research, and makes the human amniotic fluid stem cells serve as seed cells for clinical application.
Owner:NORTHWEST A & F UNIV
Method for cultivating Chinese wistaria nursery stock
ActiveCN109566232AHigh root rateShort seedling periodLayeringGrowth substratesWisteria frutescensMain branch
The invention belongs to the technical field of plant vegetative propagation, and particularly relates to a method for cultivating Chinese wistaria nursery stock. The method comprises the operation steps of overhead strip pressing, management after branch peeling, transplanting of nursery stock obtained after branch peeling and the like. Plants with flowering and fruiting seed trees and trunk diameter being 2.5 cm or above are selected, and robustly growing one-year-old lateral branches with the diameter being 0.5-1.0 cm and with no plant disease or insect pest are adopted as branch peeling branches, wherein the lateral branches germinate on the plant main branches. By adopting the overhead strip pressing method, branch peeling seedlings with developed root systems are obtained, the branchpeeling nursery stock is sheared and transplanted into a container for cultivation to obtain Chinese wistaria nursery stock. The cultivated Chinese wistaria nursery stock girdling opening rooting isfast, roots begin to grow at the 34 th day, the seedling forming stage is short, the nursery stock root system can be specially robust and strong, the transplanting survival rate can reach 91% or above, the transplanted nursery stock can grow rapidly, and after cultivation is performed for 3 months, the nursery stock can be sold. The method is simple and easy to implement, and low in cost, and theproblems that the Chinese wistaria cutting seedling rooting rate is low, the cutting seedling nursery stock is small, and time needed for flowing of the plants is long are solved. Technical supportscan be supplied for rapidly cultivating the Chinese wistaria nursery stock.
Owner:GUANGXI FORESTRY RES INST
Erythrophloeum fordii cutting propagation method
ActiveCN103460931BNarrow growth spaceResistant to lignificationHorticultureErythrophloeum fordiiDisease
The invention discloses an erythrophloeum fordii cutting propagation method which includes the procedures of stock plant selection, cutting orchard building, stock plant management, cutting slip picking, cutting and post-cutting management. Excellent robust nursery plants free of pest diseases are selected as stock plants and are densely arranged in a rectangular piece to build a cutting orchard, top cutting is conducted to promote sprouting, twigs or semi wood is selected as cutting scions which are inserted in container cups filled with substrates, and cutting seedlings are cultured to obtain excellent robust erythrophloeum fordii cutting seedlings. The method solves the problem that erythrophloeum fordii is difficult in vegetative propagation, has the advantages of being free of geographic limitation, high in propagation speed, simple, easy to operate, high in survival rate and the like, maintains good hereditary character of the erythrophloeum fordii, has great significance on protection and expansion of propagation and planting of the erythrophloeum fordii and has good economical benefit, social benefit and ecological benefit.
Owner:GUANGXI FORESTRY RES INST
Tissue culture and rapid propagation method of 1212 variety of Eucalyptus cloeziana F. Muell.
PendingCN111492978AStable genetic traitsCompletely maintain genetic characteristicsPlant tissue cultureHorticulture methodsGenetic traitsInflorescence
The invention relates to the technical field of eucalyptus cultivation, in particular to a tissue culture and rapid propagation method of a 1212 variety of Eucalyptus cloeziana F. Muell. According tothe tissue culture and rapid propagation method, excellent individual plants of Eucalyptus cloeziana F. Muell. excellent provenance are adopted, cut down and subjected to germination promoting, budlings are collected for inducing sterile buds, and regenerated plants are obtained through subculture, rooting culture and light-substrate seedlings of the sterile buds. The tissue culture and rapid propagation method of the 1212 variety of Eucalyptus cloeziana F. Muell. is low in operation difficulty, low in production cost, stable in growth of an aseptic propagation system, high in proliferation coefficient of subculture seedlings, good in repeatability and high in rooting rate of rooting seedlings, the produced tissue cultured seedlings are high in survival rate, stable in genetic traits and tidy in forest form, the seedlings can maintain the excellent genetic characteristics of excellent trees, large-area planting is suitable, and large-scale production and promotion are easy.
Owner:广西八桂种苗高科技集团股份有限公司
Method using tissue-subculture sprouts of oil-tea camellia to raise seedlings by grafting
InactiveCN101822160BGood genetic traitsReduce consumptionSeed and root treatmentClimate change adaptationCamellia oleiferaCladodes
The invention discloses a method using tissue-subculture sprouts of oil-tea camellia (Camellia oleifera Abel) to raise seedlings by grafting, particularly a method using the tissue-subculture sprout of high-grade oil-tea camellia as a scion and using the germinated germ of oil-tea camellia seeds as a stock to culture a novel plant in a shed sealed by plastic film at 10 to 35 DEG C with the air humidity being 100% on the basis of the method of cleft grafting or slope grafting, wherein the stock seeds are stored at 4 to 5 DEG C by humid sand in an icehouse having high grafting capacity and seedstorage capacity; and the grafting process is carried out day by day, and the germination acceleration is carried out group by group, accordingly, the survival rate reaches 75% to 95%. The method of the invention has the advantages of high reproductive rate, high seedling raising capacity, simple method, easy operation and low material consumption; and meanwhile, the offspring can maintain the good hereditary property of the stock plant, thus solving the problem that a large amount of improved branches and budlings are required for the existing method for the vegetative propagation of oil-teacamellia, such as cuttage propagation and seedling-raising by germ-stock grafting, and meeting the requirements of high-grade and high-quality clonal oil-tea camellia sprouts for the development of the oil-tea camellia industry in China.
Owner:GUANGXI FORESTRY RES INST
Hybrid method for improving cold resistance of Ilex rotunda
InactiveCN107711490AGood genetic traitsStable genetic traitsPlant genotype modificationGreenhouse cultivationSodium hypochlorite solution
The invention discloses a hybrid method for improving the cold resistance of Ilex rotunda. The method includes the following steps of (1) pollen storage: taking a good Ilex cornuta varfortunei plant as a female parent from April to May, using a sterilized conical flask to collect male pollen, and filling the conical flask with nitrogen for storage after low temperature baking; (2) hybridization: activating the stored pollen firstly from March to April in the second year, when pollination needs to be conducted, using a sodium hypochlorite solution and a plant extract solution to spray Ilex flowers respectively, and then conducting pollination; (3) seed treatment: placing first-generation seeds into an ammoniation pool to conduct ammoniation treatment, and then conducting temperature changing treatment; (4) repeated hybridization: according to the way of step 2, conducting hybridization cultivation on the processed first-generation seeds and Ilex cornuta varfortunei to obtain second-generation seeds, conducting hybridization cultivation on the second-generation seeds and the Ilex cornuta varfortunei to obtain third-generation seeds, and then conducting intraspecific breeding cultivation on the third-generation seeds to obtain hybridized Ilex rotunda seeds; (5) cultivation: conducting greenhouse cultivation on the hybridized Ilex rotunda seeds after the treatment of step 3.
Owner:界首市铭硕园种植专业合作社
Bending pruning seedling growing method for pearl guava
The invention relates to the technical field of agriculture plantation and especially relates a bending pruning seedling growing method for pearl guava. The method comprises following steps: a. bending pruning seedling selection; b. root promoting medium preparation; c. bending pruning: after girdling, wrapping a peeling mouth with the root-promoting media, and then wrapping the peeling mouth with rice straw and film, sawing off branches at the lower end of the peeling mouth after rooting; d. temporary planting: moving temporary planted seedlings out of the nursery after 1-2 years cultivation to obtain pearl guava planting stock. The invention provides a bending pruning seedling growing method for pearl guava, which has the advantages of convenient material obtaining, strong operability, high success rate of the bending pruning seedling growing, and keeping the excellent characters of the female parent, so as to achieve the purpose of rapidly obtaining the pearl guava seedlings for production.
Owner:广西陆川县乌坭坡珍珠番石榴专业合作社
A high-efficiency assisted selection method for molecular markers in the restorer line of japonica rice
ActiveCN109486986BObvious heterosisImprove recoverabilityMicrobiological testing/measurementBiotechnologyElectrophoreses
Owner:辽宁省水稻研究所
Tissue culture and rapid propagation method of eucalyptus cloeziana 1203 variety
PendingCN111492975AStable genetic traitsCompletely maintain genetic characteristicsHorticulture methodsPlant tissue cultureSeeds sourcePlantlet
The invention relates to the technical field of eucalyptus cultivation, in particular to a tissue culture and rapid propagation method of a eucalyptus cloeziana 1203 variety. The method comprises thefollowing steps of adopting an excellent single plant of an excellent seed source of eucalyptus cloeziana, cutting down and promoting germination of the excellent single plant, collecting bud sticks for inducing sterile buds, and performing subculture, rooting culture and container seedling culture on the sterile buds to obtain a regenerated plant. The tissue culture and rapid propagation method of the eucalyptus cloeziana 1203 variety is low in operation difficulty and production cost; a sterile propagation system is stable in growth, subcultured seedlings are high in multiplication coefficient and good in repeatability, rooted seedlings are high in rooting rate, produced tissue culture seedlings are high in survival rate, stable in inheritable character and tidy in forest form, and nursery stocks can keep excellent inheritance characteristics of superior trees and are difficult to mutate; and the method is easy for large-scale production and popularization.
Owner:广西八桂种苗高科技集团股份有限公司
A method for cultivating wisteria seedlings
Owner:GUANGXI FORESTRY RES INST
Propagation method for increasing grafting survival rate of Madhuca hainanensis Chun et How
InactiveCN102138443BImprove survival rateImproved Grafting MethodForestryHorticultureMadhuca hainanensisRootstock
The invention discloses a propagation method for increasing the grafting survival rate of Madhuca hainanensis Chun et How. The method comprises the following steps of: cutting half-lignified or lignified sun-exposed erect branches serving as scions from a Madhuca hainanensis Chun et How parent tree, removing leaves, keeping 2-4 bud eyes on each section of scion, scattering the scions, and standing for 2-3 days until myron natural juice flows out; stumping parts, which are 10 centimeters away from base parts, of 1-2 year old Madhuca hainanensis Chun et How seedlings serving as root stocks and standing for 2-3 days to make myron juice flows out naturally until incision juice is solidified; and grafting by adopting scion-root stock double side whip grafting. The Madhuca hainanensis Chun et How scions and the root stocks are pretreated, so that the problems of difficulty in operating and easily caused joint infection of a grafted plant due to excessive juice of Madhuca hainanensis Chun etHow are solved; the grafting method is improved and the scion-root stock double side whip grafting is used for grafting; and after the method disclosed by the invention is used for grafting, a wound starts to heal 8-12 days later, new buds can grow from the scions 20-25 days later, and the survival rate is increased by 22 percent and is up to 96 percent.
Owner:RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
The Group Breeding Method of Guangdong Twig Bean
ActiveCN104429971BGood genetic traitsMaintain hereditary traitsPlant tissue cultureHorticulture methodsCarrageenanEmbryo
The invention discloses a tissue culture seedling-raising method of millettia fordii dunn. The method comprises the following steps: disinfecting and sprouting seeds, selecting germinal sterile embryo axes as explants, carrying out adventitious bud proliferation, carrying out rooting culture, hardening seedlings and transplanting the seedlings to obtain millettia fordii dunn seedlings, wherein a multiplicative culture medium comprises an MS culture medium, 0.3-0.5mg / L of 6-BA, 0.06-0.1mg / L of TDZ, 0.15-0.3mg / L of NAA, 30g / L of cane sugar and 5.8g / L of carrageenan; the pH value is 5.5-5.8. The millettia fordii dunn seedlings cultured by the method has the characteristics of good hereditary stability, high reproduction speed, short seedling raising period, low cost and simplicity in operation; the experimental facility requirements are low; the operation process is simple; the millettia fordii dunn seedlings are bred in scales; the result is stable and reliable.
Owner:广州市鲸力生物科技有限公司
Molecular marker assisted efficient selection method of typical/stained abortion type restorer lines of japonica rice
ActiveCN109486986AImprove selection efficiencyObvious heterosisMicrobiological testing/measurementJaponica ricePopulation
The invention discloses a molecular marker assisted efficient selection method of typical / stained abortion type restorer lines of japonica rice. The method herein includes the steps of (1) using primers to mark first and tenth chromosome restoring genes SSR of four rice parents respectively, performing amplifying before electrophoresis to obtain restorer line genotypes of the four rice parents; (2) hybridizing the four rice parents to obtain F2-generation population restorer line gene molecular-marker-assisted selection results, and subjecting F2-genernation segregation population to molecular-marker-assisted selection to obtain a restorer line lane; (3) subjecting F4 generation to molecular-marker-assisted selection. More restore lines under wide restorer type range can be attained withina short time by molecular-marker-assisted selection; manpower, material and financial resources can be saved.
Owner:辽宁省水稻研究所
Culture medium and tissue culture method for tissue culture of Craterostigma pumilum Hochst
ActiveCN113287519AImprove survival rateGood genetic traitsHorticulture methodsPlant tissue cultureBiotechnologyCraterostigma pumilum
The invention relates to the technical field of agricultural production, and provides a culture medium and a tissue culture method for tissue culture of Craterostigma pumilum Hochst. The culture medium for tissue culture of the Craterostigma pumilum Hochst comprises a seedling breeding culture medium, a cluster bud induction culture medium, a proliferation culture medium and a rooting culture medium, wherein all the media are based on cheap and easily available MS culture media and are prepared by adding appropriate exogenous hormones or raw materials. The formula of each medium is scientific and reasonable, the media can be correspondingly used for each stage of tissue culture of the Craterostigma pumilum Hochst, the culture process is high in propagation speed, short in period and high in plant survival rate which can reach about 90%, the excellent hereditary character of the Craterostigma pumilum Hochst variety can be well kept, and market popularization and garden application of the variety are favorably promoted.
Owner:SHENZHEN XIANHU BOTANICAL GARDEN ADMINISTRATION
Cuttage rapid-propagation seedling culture method for elevated sand bed camphora wood cutting orchard tissue culture seedling twigs
ActiveCN102301883BLow costGood genetic traitsGreenhouse cultivationCultivating equipmentsBranMoisture
Owner:GUANGXI FORESTRY RES INST
Nauclea officinalis fast propagation method
Owner:海南制药厂有限公司
A method for rapid propagation of Artemisia candidum cuttage in winter
ActiveCN106069664BGrow fastHigh reproductive coefficientCultivating equipmentsSoilless cultivationShootArtemisia annua
The invention discloses a method for rapid cuttage propagation of artemisia lactiflora in winter. The method comprises the steps of 1, seedling culture facility preparation, wherein an additional layer of greenhouse film is added in a plastic multi-span greenhouse, a simple row cover is established on a seedbed, and therefore three-layers of cover film are formed; 2, cutting shoot selection; 3, cuttage, wherein cutting shoots are inserted by inclining by 45 degrees into a light medium, the cuttage depth is 3-4 cm, and bud eyes are exposed from the medium by 1-2 cm; 4, cultivation after cuttage, wherein sufficient water is provided after cuttage, moisture preservation is achieved through the covering row cover film, nutritive water is sprayed once every five days, and the dosage of nutritive water is controlled according to the principle that the water content of the medium is 60-70%. The method remarkably overcomes the defect that cuttage propagation of artemisia lactiflora seedlings can not be achieved in winter in the middle and lower reaches of Yangtze river of China, early spring transplantation and marketing of artemisia lactiflora can be conducted earlier, the growth speed of artemisia lactiflora planted with the method is high, harvesting and marketing can be conducted 30 days earlier than traditional field planting of seedlings, and economic benefits are remarkable.
Owner:NANJING INST OF VEGETABLE SCI
Multi-agent job shop negotiation scheduling optimization method
The invention discloses a multi-agent job shop negotiation scheduling optimization method. The method is divided into two stages: in the first stage, a scheme set is guided to evolve to a Pareto frontier in an inter-agent iterative negotiation mode; and in the second stage, the coordinator selects the scheme with the best social benefit from the scheme set based on the agent score. According to the method, each agent selects a part of random search schemes to put forward and form an initial parent scheme set; the coordinator performs crossover variation on the parent scheme set to generate a child scheme set, then ranks the parent and child scheme sets based on agents, and selects non-dominated schemes from the parent and child scheme sets to enter a new parent scheme set; and the negotiation is iterated for multiple times, and after a negotiation round is reached, the coordinator selects the scheme with the best social benefit from the scheme set based on the agent score. The method is based on the genetic evolution thought, and the optimization effect is effectively improved; and a more accurate information batch exposure mode is adopted, so that the social benefit is effectivelyimproved.
Owner:NORTHWESTERN POLYTECHNICAL UNIV
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