40results about How to "Good genetic traits" patented technology

The invention discloses a method using tissue-subculture sprouts of oil-tea camellia (Camellia oleifera Abel) to raise seedlings by grafting, particularly a method using the tissue-subculture sprout of high-grade oil-tea camellia as a scion and using the germinated germ of oil-tea camellia seeds as a stock to culture a novel plant in a shed sealed by plastic film at 10 to 35 DEG C with the air humidity being 100% on the basis of the method of cleft grafting or slope grafting, wherein the stock seeds are stored at 4 to 5 DEG C by humid sand in an icehouse having high grafting capacity and seedstorage capacity; and the grafting process is carried out day by day, and the germination acceleration is carried out group by group, accordingly, the survival rate reaches 75% to 95%. The method of the invention has the advantages of high reproductive rate, high seedling raising capacity, simple method, easy operation and low material consumption; and meanwhile, the offspring can maintain the good hereditary property of the stock plant, thus solving the problem that a large amount of improved branches and budlings are required for the existing method for the vegetative propagation of oil-teacamellia, such as cuttage propagation and seedling-raising by germ-stock grafting, and meeting the requirements of high-grade and high-quality clonal oil-tea camellia sprouts for the development of the oil-tea camellia industry in China.

The invention discloses a method for highly efficient assisted selection of molecular markers for japonica rice restorer lines, comprising the following steps: (1) using primers to mark the first and tenth chromosome restorer genes SSR of four rice parents respectively, and then performing amplification and electrophoresis , to obtain the restorer line genotypes of the four rice parents; (2) cross the four rice parents, and then obtain F 2 The molecular marker-assisted selection results of gene population restorer line, in F 2 The restorer line lanes of molecular marker-assisted selection in generation segregation population; (3) for F 4 generation by molecular marker-assisted selection. A large number of restorer lines and a wide range of restorer types can be selected in a short period of time by molecular marker-assisted selection, which saves manpower, material resources and financial resources.

The invention belongs to the technical field of asexual reproduction of plants, and in particular relates to a method for cultivating wisteria seedlings. The operation steps include layering in the air, management after stripping branches, transplanting of stripped branch seedlings, etc., selecting a flowering and fruiting mother tree with a trunk diameter of more than 2.5cm , The diameter of 0.5~1.0cm that germinates on the main branch of the plant is robust, and the 1-year-old side branch without diseases and insect pests is used as the stripping branch. The stripping seedlings with well-developed root systems are obtained by adopting the air layering method, and then the stripping seedlings are cut and transplanted into containers to cultivate wisteria seedlings. The invention cultivates wisteria seedlings with fast rooting at the girdling mouth, roots grow in 34 days, the seedling period is short, the root system of the seedlings is particularly strong and developed, the survival rate of transplanting can reach more than 91%, and the growth is rapid after transplanting, and it is ready after 3 months of cultivation. Can be out of the garden. The method is simple and easy to implement, has low cost, and better compensates for the problems of low rooting rate of wisteria hard branch cutting seedlings, small cutting seedlings, long flowering time of plants and the like. The invention can provide technical support for rapid cultivation of wisteria seedlings.

The invention discloses a propagation method for increasing the grafting survival rate of Madhuca hainanensis Chun et How. The method comprises the following steps of: cutting half-lignified or lignified sun-exposed erect branches serving as scions from a Madhuca hainanensis Chun et How parent tree, removing leaves, keeping 2-4 bud eyes on each section of scion, scattering the scions, and standing for 2-3 days until myron natural juice flows out; stumping parts, which are 10 centimeters away from base parts, of 1-2 year old Madhuca hainanensis Chun et How seedlings serving as root stocks and standing for 2-3 days to make myron juice flows out naturally until incision juice is solidified; and grafting by adopting scion-root stock double side whip grafting. The Madhuca hainanensis Chun et How scions and the root stocks are pretreated, so that the problems of difficulty in operating and easily caused joint infection of a grafted plant due to excessive juice of Madhuca hainanensis Chun etHow are solved; the grafting method is improved and the scion-root stock double side whip grafting is used for grafting; and after the method disclosed by the invention is used for grafting, a wound starts to heal 8-12 days later, new buds can grow from the scions 20-25 days later, and the survival rate is increased by 22 percent and is up to 96 percent.

The invention discloses a tissue culture seedling-raising method of millettia fordii dunn. The method comprises the following steps: disinfecting and sprouting seeds, selecting germinal sterile embryo axes as explants, carrying out adventitious bud proliferation, carrying out rooting culture, hardening seedlings and transplanting the seedlings to obtain millettia fordii dunn seedlings, wherein a multiplicative culture medium comprises an MS culture medium, 0.3-0.5mg / L of 6-BA, 0.06-0.1mg / L of TDZ, 0.15-0.3mg / L of NAA, 30g / L of cane sugar and 5.8g / L of carrageenan; the pH value is 5.5-5.8. The millettia fordii dunn seedlings cultured by the method has the characteristics of good hereditary stability, high reproduction speed, short seedling raising period, low cost and simplicity in operation; the experimental facility requirements are low; the operation process is simple; the millettia fordii dunn seedlings are bred in scales; the result is stable and reliable.

The invention discloses a cuttage rapid-propagation seedling culture method for elevated sand bed camphora wood cutting orchard tissue culture seedling twigs. The method comprises the following steps: taking semi-lignified twigs formed by camphora wood cutting orchard tissue culture seedling planted on the elevated sand bed as a cutting wood; treating the cutting wood by greenplant growth regulator-ABT6 rooting solution; inserting the treated cutting wood into a mixed medium which contains peat soil and coconut bran in a weight ratio of 3:(1-1.5); culturing on a pressure lifting seedling culture bed capable of automatically controlling temperature, controlling moisture and controlling light; controlling parameters of air humidity to 85%, medium moisture content to 50% and illumination intensity to 1500lux; and after one month, causing the cutting wood to form an integral plant, wherein the cuttage survival rate is 80-85%. The method has the advantages of scientific seedling culture facility, high propagation speed, large seedling culture amount, short seedling culture time, high afforestation survival rate, low wood culture cost, good economic benefit and social benefit and the like, offspring can keep the excellent hereditary character of a stock plant, and the asexual seedling culture of camphora fine variety is achieved.

The invention belongs to a propagation method of south China medicinal plants (Li medicinal plants), in particular to a nauclea officinalis fast propagation method. The method comprises the following steps: selecting sandy loam land blocks for carrying out soil tillage; then, carrying out top pruning bud saving after seed seedlings are planted, wherein each parent strain maintains 2 to 3 strains of healthy and strong lateral buds; placing nutritional bags with nutritional soil on furrows in a seedling green house in lines; taking naphthylacetic acid to be diluted by warm water to obtain rooting liquid; placing the rooting liquid to the normal temperature for use; selecting the lateral buds on the parent strains; then, soaking base parts of the lateral buds in the rooting liquid; sticking the soaked base parts of the lateral buds on the nutritional bags; then, covering an agricultural use film for heat preservation and moisture preservation; carrying out culture; and carrying out seedling emergence and transplanting when the seedlings grow seven pairs of true leaves and the height is higher than 20cm. The invention adopts healthy and strong burgeons for sticking culture, the root system growth can be effectively promoted, the survival rate can be improved, the growing speed of the plants can be improved, the resistance of the plants can be enhanced, good hereditary features of the parent plants are perfectly maintained, and the invention provides a high-efficiency path for the development of the nauclea officinalis planting industry, and is favorable for the development andutilization of the south China medicinal plant (Li medicinal plant) resources.

The invention discloses a method for rapid cuttage propagation of artemisia lactiflora in winter. The method comprises the steps of 1, seedling culture facility preparation, wherein an additional layer of greenhouse film is added in a plastic multi-span greenhouse, a simple row cover is established on a seedbed, and therefore three-layers of cover film are formed; 2, cutting shoot selection; 3, cuttage, wherein cutting shoots are inserted by inclining by 45 degrees into a light medium, the cuttage depth is 3-4 cm, and bud eyes are exposed from the medium by 1-2 cm; 4, cultivation after cuttage, wherein sufficient water is provided after cuttage, moisture preservation is achieved through the covering row cover film, nutritive water is sprayed once every five days, and the dosage of nutritive water is controlled according to the principle that the water content of the medium is 60-70%. The method remarkably overcomes the defect that cuttage propagation of artemisia lactiflora seedlings can not be achieved in winter in the middle and lower reaches of Yangtze river of China, early spring transplantation and marketing of artemisia lactiflora can be conducted earlier, the growth speed of artemisia lactiflora planted with the method is high, harvesting and marketing can be conducted 30 days earlier than traditional field planting of seedlings, and economic benefits are remarkable.