The invention discloses a molecule marking method of a rice blast-resisting
gene, belonging to the field of
crop molecule
heredity and breeding. The method comprises the following steps: (1) taking a rice sample and extracting a
genome DNA (
Deoxyribose Nucleic Acid) of the rice sample; and (2) carrying out PCR (
Polymerase Chain Reaction) amplification on the
genome DNA of the rice sample by utilizing any one pair of molecule-marked primers in RM6091 and RM26632, carrying out
electrophoresis detection on a PCR amplification product, and if a molecule-marked
DNA segment with corresponding size is amplified, showing that a Pi-bdl(t)
gene exists. Through rice blast-resisting
gene Pi-bdl(t) molecule marking in the invention, whether thin rice as well as crossbred descendants, backcross descendants and multiple cross descendants thereof contain the gene can be detected, the resistance level of the gene on rice blast can be forecasted, the selecting efficiency of rice blast resistant materials can be greatly increased, and the breeding process for
disease resistance can be accelerated.