461 results about "Selfing" patented technology

The invention discloses a method for transforming a hot-pepper nucleo-cytoplasmic interactive male-sterile line, a homo-maintainer line and a homo-restorer line, which comprises the following steps of: hybridizing by using a hot-pepper selfing line as a male parent and the hot-pepper nucleo-cytoplasmic interactive male-sterile line as a female parent, simultaneously selfing a male parent plant, selecting sterile plants or other inflorescences in 4-50 corresponding male-parent filial single plants and hot-pepper filial generations for paired test crossing when in filial-generation fertility separation, selfing each male-parent single plant, and respectively processing according to the group fertility expression of test crossing generations to select the male-sterile line, the homo-maintainer line and the homo-restorer line. When in filial-generation group fertility separation, the invention not only can be used for transforming the hot-pepper nucleo-cytoplasmic interactive male-sterile line and the homo-maintainer line from the hot-pepper selfing line, but also can be used for breeding the homo-restorer line, expands the selecting space of the restored male parents of other homologous sterile lines and can be used for screening more excellent three-line combined varieties. A hot-pepper hybrid is produced by adopting a male-sterile three-line technology, all the complex pollination procedures are simplified, and the economic benefit is quite remarkable.

The invention provides a five-in-one variety breeding method. The five-in-one variety breeding method comprises the following steps: firstly, taking a hybridized combination A*B or a population variety C as a base material, planting the base material in a strong stress environment at high density and large group, continuously carrying out selfing and test mating by using an IPT ((Isopentenyl-Transferases) method, planting the test mating progenies in regions with remarkably different ecological environments and selecting single plants D of which the test mating progenies meet certain standard; secondly, carrying out DH (Doubled Haploid) breeding by taking the D as the material to obtain more than 1000 haploid seeds, doubling the haploids to obtain more than 100 DH pure lines, carry outing target character selection, genetic background restoration selection and homozygosity identification by using an MAS (Marker Assisted Selection) molecule marker assisted breeding technology and selecting a DH pure line F according with a breeding target; thirdly, taking F as parent and assembling the F with the traditional common pure line cultivar to form a hybridized combination, planting the hybridized combination at more than ten test points in different ecological regions and selecting hybridized combination with strong adaptability, high quality and high yield as a hybrid variety G for breeding. According to the five-in-one variety breeding method provided by the invention, the breeding efficiency is increased.