Continuous recessive character backcrossing technology
A technology of recessive traits and technology, applied in the field of continuous backcross technology for recessive traits, can solve problems such as insufficient breeding technology routes
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Embodiment 1
[0040] Example 1. refer to figure 1 . A continuous backcross technique for recessive traits, the steps of which are as follows:
[0041] (1) The material to be improved without the target recessive gene is used as the recurrent parent, and its genotype is AA, and the material containing the target recessive gene aa is used as the donor parent; the recurrent parent is used as the female parent to cross with the donor parent, Or prepare a backcross combination to harvest the first generation of hybrid F 1 with reincarnated parent seeds;
[0042] (2) Planting hybrid first generation F 1 Seeds with the reincarnated parent, with the reincarnated parent as the female parent and the first generation of hybrid F 1 Crossbreed to generate backcross generation BC 1 f 1 Seed, Harvest Backcross Generation BC 1 f 1 with reincarnated parent seeds;
[0043] (3) Planting backcross generation BC 1 f 1 Seeds with the reincarnated parent, with the reincarnated parent as the female par...
Embodiment 2
[0047] Example 2. In step (4) of embodiment 1, from BC 1 f 2 In the process of selecting recessive traits, single plants are crossed with recurrent parents, and BC is harvested in pairs at the same time 2 f 1With the reincarnation parent seed, as a backup branch.
Embodiment 3
[0048] Example 3. A kind of creation method of the purple leaf marker isogenic line of the continuous backcrossing technology of the recessive character described in application embodiment 1, its steps are as follows:
[0049] (1) The material to be improved without the purple leaf trait is used as the reincarnation parent, and the purple leaf material is used as the donor parent;
[0050] Use the recurrent parent as the female parent to cross with the donor parent, or prepare a backcross combination; harvest the first generation of hybrid F 1 with reincarnated parent seeds;
[0051] (2) Planting hybrid first generation F 1 With the seeds of the reincarnated parent, cross the first generation of hybrids with the reincarnated parent as the female parent to produce the first generation of backcrossed seeds; harvest the first generation of backcrossed BC 1 f 1 with reincarnated parent seeds;
[0052] (3) Planting backcross generation BC 1 f 1 With the seeds of the recurren...
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