562 results about "Oryza" patented technology

The invention discloses a seed coating agent specially used for direct seeding rice, which is characterized in that the seed coating agent mainly contains the following ingredients by weight: 2 to 8 percent of super absorbent resin, 1 to 5 percent of oxygenates, 0.1 to 0.5 percent of sparrow and rat destruction agent , 0.5 to 2 percent of bactericide, 0.5 to 2 percent of pesticide, 0.02 to 0.2 percent of plant growth regulator, 3 to 10 percent of trace element fertilizer and the margin is special type mineral clay. The powder raw materials are crushed to more then 100 meshes and are mixed evenly, the mixture is coated on the surfaces of rice seeds by a seed-coating method for direct sowing, thus clay sacculus integrating 'a small water reservoir, a small oxygen reservoir, a small drug storage and a small fertilizer storage' are formed around the rice seeds and at the roots of seedlings; the seed coating agent has the effects of preserving and providing water, ventilating and providing oxygen, promoting seedling emergency, preventing diseases and killing pests, preventing mice and destructing sparrows, strengthening seedlings and roots and increasing production. The seed coating agent is also characterized by low dosage, low cost, simple and convenient operation, and the like, thereby being applicable to coating rice seeds which are directly sowed both on flooded paddy fields and dry paddy fields.

The invention relates to a cultivating technique of cross bred lobster in rice field and pond. The technique is the producing manner which utilizes the shallow water environment in the rice field and is supplemented by artificial measures to plant the rice and cultivate the lobster to increase the profit of the unit rice field area. Since the freshwater cross-bred lobster has low requirements on water and the cultivating place, and fish culture in paddy field is a tradition in a plurality of areas in China, and the economic profit of the unit rice field area can be effectively increased by popularizing the culture of the freshwater cross-bred lobster in the rice field when the profit of fish culture is dropped. The culture of the freshwater cross-bred lobster in the rice field can facilitate weeding and pest eliminating in rice field and save fertilizer and pesticide; and the rice yield of the rice field raising the lobster can be increased by 5percent to 15percent generally. In some areas, in particular the areas of waterlogged lowland, winter macerated fallowland and cold water paddy field, where only single cropping is allowed, the mode of the rice planting and the freshwater cross-bred lobster cultivation in turn is adopted and the economic profit is remarkable.

The present invention discloses a paddy field rail duck raising technology. Rice, insect, duckweed and duck constituting one biological chain are made to control each other; the time and the space for the four kinds of organisms to constitute the complex colony are controlled based on the rice growth rule and the symbiotic biological environment for rice and duck is improved to realize the pollution-less rice plantation and duck raising. By stimulating natural biological system, the duck raising in paddy field can control the harmful disease, pest and weed, reduce pesticide amount, produce green food material and increase rice field output value.

The invention relates to an artificial feed of sesamia inferens walker as well as a preparation method and a feeding method thereof, belonging to the technical field of insect feeding. The artificial feed comprises soybean meal, corn meal, wheat germ, yeast powder, casein, cane sugar, honey, fresh wild rice shoots or fresh rice stems, ascorbic acid, cholesterol, choline chloride, Fiske salt, vitamin B complex, vitamin E, sorbic acid, methyl parahydroxybenzoats, 40 percent formaldehyde, 98.18 percent rice ketone, agal-agal and water. A long-term and successive feeding method of the sesamia inferens walker is carried out by adopting a wild rice shoots (young larva) and artificial feed (old larva until pupation). The fed larva of the sesamia inferens walker have development period of 24.8 days, larva survival rate of 76.6 percent, percentage of pupation of 87.7 percent, eclosion rate of 91.3 percent, egg laying amount of 101.9 for single adult and egg hatching rate of 88.8 percent. The invention has the advantages of low cost, practicability, simple preparation, and the like, and is suitable for massive, long-term and successive feeding of standard test sesamia inferens walkers.

The invention discloses a method for breeding functional formula rice varieties, belonging to the technical field of special rice breeding. The method is characterized by comprising the following steps of selecting a rice variety with larger extending area and good quality in production as the recurrent parent, crossing the recurrent parent with black rice, purple rice, red rice, glutinous rice and giant embryo rice respectively, backcrossing the offsprings with the recurrent parent, breeding the near-isogenic lines which have agronomic traits similar to the agronomic traits of the recurrent parent and respectively have the traits of the black rice, purple rice, red rice, glutinous rice and giant embryo rice and mixing the seeds in proportion to prepare the seeds for production of the formula rice. The method has the following beneficial effects that after the formula rice varieties bred by the method are planted, the shapes of plants and leaves and the agronomic traits are neat and consistent; the rice obtained by grinding unhulled rice can simultaneously contain the five functional rice, namely the black rice, the purple rice, the red rice, the glutinous rice and the giant embryo rice; the functional formula rice can be produced by planting the seeds; the production method is simple and is high in efficiency and good in benefits; and the produced high nutrition functional formula rice integrates color, aroma, taste and nutrition curative effect and has good health care function.

The present invention relates to methods of reducing chemical fertilizer usage and greenhouse gas nitrous oxide emission and to methods of improving plant growth rate and seed productivity in agriculture through the application of a novel artificially manufactured formula containing a nitrogen-fixing bacterium that efficiently colonizes non-legume plants in aerial parts and the root system. The bacteria inocula and methods are particularly suitable for plants in the genera Jatropha, Sorghum, Gossypium, Elaeis, Ricinus, Oryza and Manihot.

The invention relates to a method for high-efficiently cultivating excellent later generation of the wild rice distant hybridization , which is to cultivate the wide rice distant-hybridization high-efficient excellent later generation through the hybridization of the cultivated rice, the embryo rescue, the strong seedling culture, the pollen culture and the molecular marker assisted selection of the hybridization later generation of the cultivated rice and the wide rice. The survival rate of the distant hybridization of the invention is high; the hybrid progeny is backcrossed at the preliminary period and then self-bred, thus being beneficial to overcome the reproductive barrier caused by the different genome in the hybrid progeny of the wide rice and the cultivated rice, and to stabilize more genetic characters of the wide rice inside the cultivated rice; the pollen culture can accelerate the stability of the purification of the hybrid progeny; with the established molecular marker assisted selection, the breeding can be accurately performed without being influenced by the environmental conditions and being restricted by the growth and development period.

The invention particularly relates to a breeding method for producing paddy rice with a colorful rice producing function and belongs to the field of special rice breeding. The breeding method comprises the following steps of: (1) selecting a three-line maintenance line B as a female parent to be hybridized with a black rice male parent, carrying out multi-generation backcrossing on the first filial generation and the maintenance line B, and breeding a maintenance line black B with black seed coats and with other properties the same as the maintenance line B; (2) using a sterile line A corresponding to the three-line maintenance line B to be hybridized with the maintenance line black B to produce a sterile line black A containing heterozygous black seed coat genes; (3) using the sterile line black A to be hybridized with a restore line endosperm R to produce first filial F1 generation seeds filial F1; and (4) using the first filial F1 generation seeds filial F1 as production seeds. Thebreeding method has the advantages that hybridized paddy rice seeds capable of being directly used for producing colorful rice can be produced through the breeding method, and the functional colorfulrice can be produced through planting the seeds. The production method is simple, the efficiency is high, the benefits are good, and the produced rice is rich in various nutrition elements.

The invention relates to a crayfish rice continuous-cropping breeding and planting technology. The invention has the special technical key points that: the rice field, which has good water quality, sufficient water and no pollution source and is convenient to irrigate and drain, is selected to breed crayfish; the area of the rice field is properly over dozens of acres; feeding ditches are excavated in ridges; a crayfish rice continuous-cropping breeding and planting mode comprises three modes of throwing seed crayfish, throwing ovigerous crayfish and throwing young crayfish; according to the requirements of the three modes, the seed crayfish or the young crayfish are thrown sufficiently in one step; when a large number of young crayfish move, artificial feed such as minced fillet, scrambled screw clam meat and the like are fed properly; in winter, crayfish enter caves to live through the winter; in March of the next year, the crayfish come out again after water temperature rises again, wherein the water temperature is controlled by a method for adjusting water depth and is controlled to be 20 to 30 DEG C commonly; and the crayfish are caught at the end of March to at the end of May every year. By the technology, the rice field can be utilized completely to artificially cultivate the crayfish; the problem that the rice field leaves unused in rainy seasons of winter and spring is solved; and a wide prospect for farmers to achieve prosperity is achieved.

The invention discloses a mixing breeding method of fish and ducks in a paddy field and relates to a comprehensive utilizing method of planting and breeding, which comprises the following steps: (1) preparing the paddy field, ditching a ditch with the depth of 50-120cm and the width of 30-120cm in the paddy field and digging a pit with the depth of at least 120cm, the width of at least 180cm and the length adapted to the length of the south end of the paddy field at the south end of the paddy field, wherein a pit opening is as high as the bottom surface of the ditch; filling water till water height is 10-30cm above than the surface of the paddy field; (2) three days after seedlings are planted in the paddy field, breeding 15 to 18 ducklings per mu at the breeding time between sunrise and sunset each day; (3) five days after the seedlings are planted in the paddy field, breeding 50-150 kilograms of fish fries per mu once; (4) before harvest, draining the water in the paddy field, leading all fishes to enter the pit and acquiring the fishes; (5) after the fishes are acquired, breeding a duck every day; after rice is harvested, keeping breeding the duck every day and feeding rice grains left in the field; and after the rice grains are eaten up by the duck, acquiring the duck. The invention improves the utilization benefits of the farmland and increases the economic income of farmers.

The invention discloses a plant source composition. The plant source composition comprises the following ingredients: saccharomycopsis ferment filtrate, nicotinamide, panthenol, sodium ascorbyl phosphate, arbutin and a compound extract, wherein the compound extract comprises radix cynanchi atrati extract, chlorella extract, rice germ extract, barley malt extract, vaccinii myrtilli folium extract,punica granatum fruit extract, fructus hippophae extract, sesame sprout extract, wild soybean sprout extract, wheat malt extract, tomato fruit extract and aloe barbadensis leaf extract. The plant source composition further comprises the following ingredients: a conditioner, a humectant, a pH regulator, an emollient, a solubilizer, a solvent and a chelating agent. The plant source composition has the cooperative efficacies of whitening the skin, preserving moisture and resisting oxidation, the essence has fine and smooth texture, and the active ingredients can be easily absorbed by the skin.

The present invention discloses breeding process of rice blast-resisting high quality and high yield round-grained rice variety Zhendao-10. The breeding process breeds rice variety featuring high resistance to rice blast, high quality and high yield. The breeding process includes the following steps: 1. hybridizing Zhendao-99 hybrid rice variety with early maturing, high yield and good taste as the female parent and Zhen-540 hybrid rice variety with excellent appearance quality as the male parent to obtain F1; and 2. selfing with the F1 for six generations to obtain the rice blast-resisting high quality and high yield round-grained rice variety Zhendao-10.

A rice and lobster co-culture method includes a rice planting part and a lobster breeding part. The lobster breeding part includes the steps of (1) rice field modification; (2) juvenile shrimp culture; (3) adult shrimp breeding; (4) parent shrimp putting; (5) adult shrimp fishing, supplementary juvenile shrimp putting and parent shrimp retention. The rice planting part includes the steps of (1) rice cultivation; (2) rice field management; (3) field drying; (4) rice pest and disease control; (5) draining and harvesting. Compared with an ordinary rice and lobster co-culture mode, the morbidity frequency of lobsters can be reduced to 45 days or so from 7-10 days, the disease control cost is reduced by 150 yuan / mu or so, and the survival rate can be increased to 80% or above from 30-40%.

The invention provides a culture technology of perennial regenerative rice, which displays advantages by the following: treating in accordance with local conditions and choosing high-quality seed; choosing region and farm land; cultivating, reseeding and fertilizing scientifically timely; managing water scientifically and dormant baked the farm land timely; preventing and wiping out plant diseases and insect pests and birds and rats; harvesting timely and collecting cereal carefully. The invention is characterized by the following: saving labor and cost and early-ripening (using seasons to culture double-cropping paddy); having high and stable yield; being like rice transplanting rice in grain size and bigger than current regenerative with stable culturing; extending high-quality seed to degenerate; adjusting labor stress at busy time and tension in supply of the high-quality seed. The invention improves the yield through using the technology to culture the perennial regenerative rice efficiently, which can achieve about 505. 2kg per mu and the most being 572. 8kg per mu and the yield of two seasons can be more than 900kg or even be a ton of grain per mu If cultures the perennial rice in last season while rice transplanting rice in next season.

The present invention relates to active compound combinations, in particular within a fungicide composition, which comprises (A) prothioconazole and (B) fluxapyroxad and optionally (C) a further fungicidally active compound. Moreover, the invention relates to a method for curatively or preventively controlling the phytopathogenic fungi of plants or crops (e.g. cereals such as wheat, barley, rye, oats, millet and triticale; soya beans; rice; com / maize; oil seed rape including canola; beans, peas, peanuts; sugar beet, fodder beet, beetroot; potatoes; cotton), and to the use of a combination according to the invention for the treatment of seed, to a method for protecting a seed and not at least to the treated seed.

The present invention provides polyploid rice selectively breeding and identifying method with polyploid meiosis stability (PMeS) gene. The rice selectively breeding process includes: selecting parents; hybridization or complex hybridization; back crossing; doubling chromosome; identifying polyploid; selecting bearing property; observing meiosis behavior; self-crossing to form stable line; and identifying high bearing property. The identifying indexes include: polyploid line of Asia cultivated indica type rice or japonica rice; bearing rate not lower than 65 %; normal meiosis behavior; normal pollen shape and physiological activity and normal dyed pollution with 0.1 5 I-KI not lower than 95 %; bearing rate of hybrid of PMeS polyploid rice and other polyploid rice variety over 70 % and that of PMeS polyploid rice and wild rice variety over 65 %. The present invention solves the problem of low bearing rate of polyploid rice and may be used in breeding polyploid rice variety.

A rice cultivar designated Templeton is disclosed. The invention relates to the seeds of rice cultivar Templeton, to the plants of rice cultivar Templeton, to plant parts of rice cultivar Templeton, and to methods for producing a rice plant produced by crossing rice cultivar Templeton with itself or with another rice variety. The invention also relates to methods for producing a rice plant containing in its genetic material one or more transgenes and to the transgenic rice plants and plant parts produced by those methods. This invention also relates to rice cultivars, or breeding cultivars, and plant parts derived from rice cultivar Templeton, to methods for producing other rice cultivars, lines or plant parts derived from rice cultivar Templeton, and to the rice plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid rice seeds, plants, and plant parts produced by crossing rice cultivar Templeton with another rice cultivar.

The invention discloses a rice cultivation method. The rice cultivation method comprises the following steps of: S10, preparing before seeding; S20, carrying out seeding operation; S30, carrying out irrigation by infiltration on a rice field in a whole growth period when the rice enters the growth period, and carrying out pest control and grass disaster control; and S40, timely harvesting according to the rice growth period and carrying out planting on next round of crops. According to the rice cultivation method disclosed by the invention, no-tillage direct seeding and irrigation by infiltration are organically combined, so that the no-tillage direct seeding saves a rice seedling bed period; and flooding irrigation is replaced by the irrigation by infiltration, so that the technical problems that the conventional rice cultivation technology is high in seedling transplanting and greenhouse gas discharging amount, and lower in fertilizer and moisture utilization efficiency are solved, energy-saving and emission-reducing capacity of a rice producing system is improved, and purposes of saving the oil consumption by 45%, comprehensively reducing emission of the greenhouse gas by 50% and remarkably improving the water fertilizer utilization efficiency are achieved; and moreover, the rice cultivation method is higher in mechanical degree, simplified in technology, easy to popularize, and especially has popularization prospect in a rice-wheat (oilseed rape) paddy-upland rotation region.

The invention discloses a frog-rice stereoscopic culturing technology. The production procedure the steps of comprehensive utilization planning, seedling culturing and fingerling stocking, cultivation management and daily management. According to the frog-rice stereoscopic culturing technology, the planting industry and the cultivation industry are efficiently combined, on the same piece of a paddy field, rice can be planted, the frogs can also be cultivated, and harvesting of both the rice and frogs is achieved; the culturing environment is superior, the frogs can be cultivated simply by digging field facilities like fish ditches and fish pits and maintaining the stable water temperature, besides, dissolved oxygen in water is sufficient, and water often flows and exchanges; the frogs in the paddy field can eat pests in the field, pesticide does not need to be sprayed to the rice, faeces of the frogs can provide nutrients for the rice, and chemical fertilizer does not need to be applied, which is beneficial to improvement of the health level of people; meanwhile, the frogs unceasingly move and forage in the paddy field, which loosens the soil, makes water flow and ventilates for the paddy field, rich and diversified products can be provided, the employment of surrounding labor force is driven, and by popularizing the frog-rice stereoscopic culturing technology, surrounding farmers are driven to become rich.

The invention relates to a hybrid rice single-parent dense planting machine transplanting cultivation method. Hybrid rice seeds which do not germinate are removed through a photoelectric color sorter, and germination rate of sown seeds is larger than or equal to 98%. A manual seeder or a printing seeder is adopted to position single-grain seeds for seedling raising, a rice transplanter is used for high-density single-parent seedling machine transplanting, transplanting density is 24-25 thousand holes for double cropping rice and is 18-20 thousand holes for single cropping rice, and high-yield management measures are adopted for field management after greening up. By the method, seed cost can be saved, strong seedlings can be cultivated, yield can be increased remarkably, and comprehensive benefit of rice planting can be increased.

The invention relates to a method for preventing and removing rice weeds and weeds in direct seeding and interplanting seeding paddy, which pertains to a technical field of plant protection. The invention is mainly characterized in that: rice weed seeds and weed seeds left during direct seeding and interplanting seeding of last year are induced to grow; after former planted paddy is harvested, Glyphosate, a spray sterilant herbicide, is employed to prevent rice weeds and weeds, and solving the problem of incapability of removing older green weeds left during former planting paddy in direct seeding and interplanting seeding paddy; meanwhile, the Glyphosate has no effect on germination and growth of planted rice seeds and is environment friendly. The method for preventing and removing of the invention overcomes the shortages of incapability of employing chemical herbicides to remove rice weeds, worse and worse influence to normal direct seeding paddy, interplanting seeds, etc., and even destruction of cultivation and repeated displanting. The method for preventing and removing is in favor of paddy production in direct seeding and interplanting seeding and has significant social benefits.

The invention discloses a frog-rice co-culture ecological high-yield breeding-planting method. The method includes: building a spawning pond: arranging rectangular-ambulatory plane trenches in the pond, and arranging food intake areas around the spawning pond and rest land in the middle; releasing seed frogs into the spawning pond according to a male-female ratio of 2:1, and transferring the seedfrogs into an incubation pond for incubation; building a rice field pond: arranging food intake areas around the rice field pond, trenching around the food intake areas, and forming a rice planting area in the middle; releasing tadpoles: releasing the tadpoles into the rice field pond according to density of 500 tadpoles per square meter; planting rice: in the middle of May which is the seedling culture and seeding period in Hubei Province, planting 11-13 thousand plants per mu, and placing shrimp cages in the food intake areas on September in Hubei Province to catch frogs; manually harvesting. The method has the advantages that conditions for co-growth and co-development of frog and rice are improved reasonably, one-field multi-purpose, one-water multi-purpose and one-season multi-harvestdouble high-yield effect is realized, sustainable and sound development of agricultural production is realized, and pure benefit per mu is more than 11000RMB.

The invention belongs to the field of genetic engineering of plants and discloses a method for building a high-efficiency regenerating and transforming system of Oryza sativaL. subsp. japonica 11 through cultivation of induction of embryogenic callus, subculture multiplication of embryogenic callus, transformation mediated by agrobacterium tumefaciens, cocultivation of embryogenic callus and agrobacterium tumefaciens, screening and cultivation of resistant callus and seedlings differentiation of resistant callus. The method has the advantages of simple technological processes, low production cost, and capabilities of obviously increasing the inductivity of the embryogenic callus, the subculture multiplication rate and the differentiation and regeneration rate of the embryogenic callus of the Oryza sativaL. subsp. japonica 11 and simultaneously shortening the period of obtaining regenerative rice plants; and on the basis, an improved agrobacterium tumefaciens-mediated method can be used for remarkably increasing the efficiency of obtaining the transgenosis rice plants and shortening the period of obtaining regenerative rice plants.

The invention discloses a method for breeding colored rice two-line hybrid rice. The method comprises the steps of carrying out hybridizing by taking colored rice +C as a male parent and a two-line sterile line S as a female parent, and carrying out backcrossing with the female parent to obtain BCnF2 generation seeds; selecting male sterile plants, carrying out paired test cross on the male sterile plants and a two-line restoring line R, and selecting and maintaining homozygous sterile line plants with colored seed coats and corresponding hybrid seeds; detecting seed heterosis, and selecting and maintaining hybrid plants and sterile line plants with heterosis to obtain colored rice sterile line seeds; identifying the fertility, yield, quality and resistance of the sterile line to obtain excellent colored rice sterile line plants; and breeding seeds to obtain a colored rice two-line hybrid system. According to the method, the colored rice and the two-line sterile line of the approved hybrid rice are hybridized and backcrossed, and the two-line sterile line plants with the colored seed coats and heterosis are screened through the restoring line, so that the obtained colored rice hybrid rice and the sterile line thereof not only have the colored seed coats, but also have the advantages of high yield, high quality and disease resistance.

The invention relates to a rice-fish comprehensive planting and breeding technical method. The method is a production method of cultivating and planting ratoon rice after harvesting of single-season rice and continuing fish stocking in the rice field by the utilization of the ecological principle of rice fields. According the ratoon rice, after ripening of the single-season rice, about upper 2 / 3 parts of rice plants are left, ears of rice are picked and the lower 1 / 3 part of the plants and roots is left; after fertilization and cultivation, the second season rice grows out. Breeding of fish inthe rice field runs through the whole process. Melons and vegetables are planted in the ridge, thus creating the three-dimensional circulating rice-fish comprehensive planting and breeding model of ''single-season rice+ ratoon rice + fish'', namely the ''single-season rice+ ratoon rice + fish'' model. The technical method of the invention can be promoted and applied in rice fields in mountainousareas; the bred Sanjiang rice field carp contains rich selenium, which is very beneficial to the human body; and good economic, social and ecological benefits can be obtained.

The invention relates to a rice-shrimp intercropping symbiotic method of single-season rice and three-season shrimps, and belongs to the technical field of ecological planting and breeding. The method comprises the steps of digging a circular furrow at the periphery of a farmland with the area of 50-60 mu, and then planting rice and water weeds in the farmland in the water weeds in an interval manner. Through building a rice field comprehensive planting and breeding ecological system, biological bait resources in the rice field can be fully utilized, the rice and the shrimps grow together, and the problems that a lot of pesticide is used for rice planting, environment is polluted by fertilizer, people health is also influenced, genetic resources are degenerated and yield is decreased due to overfishing of wild crayfish resources and backward artificial breeding technology are solved. The purposes of keeping ecological balance and increasing planting and breeding benefits are achieved.

The invention discloses a grain-rapeseed double-harvesting planting method for a red-soil rice field. The method comprises the steps: (1) selecting a middle-season rice and ratoon rice variety, planting middle-season rice in a red-soil region in a rice seedling transplanting or throwing manner at the first ten days of May, and reaping the middle-season rice at the last ten days of August; and carrying out stubble-remaining regrowing so as to obtain ratoon rice, strengthening field management, and reaping the ratoon rice at the last ten days of October; and (2) selecting a hybridized double-low rape variety, and carrying out interplanting 5 to 7 days before reaping the ratoon rice at the last ten days of October; and carrying out mechanical harvesting and threshing when rape pods of an entire rape field are yellow and seed coats are black brown from the end of April to the beginning of May in an ensuing year. According to the method, on one hand, high and stable yielding of rice and rape can be simultaneously guaranteed from planting time; meanwhile, the middle-season rice is planted in advance by 20 to 30 days (the first or middle ten days of May) in this research, so that the condition that the ratoon rice is reaped at the end of October is ensured, thus, sufficient planting time for rape planting is set aside, and the planting of the middle-season rice is not affected after the rape is reaped; and bumper harvest in both grains and rapeseed is expected.

The invention discloses a method for increasing breeding efficiency of wild rice and cultivated rice distant hybridization embryo rescue, belongs to the field of breeding by the biological technique, and aims at reducing the alcohol disinfecting time and reducing the alcohol concentration as well as creating foundation for the survival rate of hybridization seedlings. The method is that the MS of an embryo culture is improved; the concentration of KNO3, NH4NO3, KH2PO4 and MnSO4.4H2O is reduced; meanwhile, CuSO4.5H2O, CoCl2.6H2O, niacin, pyridoxine hydrochloride and thiamine hydrochloride are not added; the hardening-seedling technology is innovated; when in hardening seedling of the plants, the hardening-seedling treatment with convenient operation and good effect is carried out, so that the survival rate of the seedlings can be greatly increased. According to the operation of the method, the operation of embryo rescue is simplified; the whole culture costs 30 days only, so that the culture cycle can be greatly reduced; meanwhile, the seedling rate can be greatly increased and up to 94%; therefore, the breeding efficiency of the wild rice and cultivated rice distant hybridization embryo rescue can be increased.

The invention discloses a bryophyte reversal-resistant protein PpLEA3-20 and an encoding gene and an application thereof. A plant resistance-related protein provided by the invention is named PpLEA3-20, is derived from physcomitrellapatens, and is a protein shown as (1) or (2), wherein (1) is constituted by an amino acid sequence shown as a sequence 2 in a sequence table, or (2) is obtained by performing substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid residue sequence shown as the sequence 2 in the sequence table, is relevant to plant resistance and is derived from (1). As proved by an experiment for introducing the encoding gene of PpLEA3-20 into oryza sativassp.japonica, the survival rates (44 percent, 36 percent, 60 percent and 72 percent) and growth states of four obtained T2-generation PpLEA3-20 transgenic plants (20-1, 20-2, 20-3 and 20-4) in a drought stress experiment are remarkably better than those of a check plant.

The invention belongs to the technical field of cross breeding of plants and relates to a novel method for improving an achievement rate of a first backcross hybrid generation of oryza sativa and oryza minuta. The method includes that firstly, the oryza sativa is used as a female parent, the oryza minuta is used as a male parent, and the oryza sativa and the oryza minuta are subjected to hybridization and embryo rescue to obtain an interspecific hybrid F1 plant; secondly, the interspecific hybrid F1 plant is used as a female parent, the corresponding oryza sativa is used as a male parent, the interspecific hybrid F1 plant and the corresponding oryza sativa are subjected to backcross, by means of a method which is similar to a method of hybrid rice seed production, and the pollination is directly performed for 3 consecutive days to obtain a plurality of young embryos of a first wide cross and backcross hybrid generation; and finally, the plurality of young embryos are subjected to the embryo rescue to obtain the first backcross hybrid generation BC1F1 plant. According to the novel method for improving the achievement rate of the first backcross hybrid generation of the oryza sativa and the oryza minuta, the method is simple in operating and low in cost, the achievement rate can be stably improved from current 0.02% -0.03% to 0.32%-0.35%, and the problem that the first backcross hybrid generation BC1F1 of the oryza sativa and the oryza minuta is difficult to achieve is solved.