The invention relates to an artificial cultivation and
seedling industrial production method of a
dendrobium nobile
polyploid compound, which comprises the following steps of: carrying out artificial
pollination in a flowering period of common
dendrobium nobile, inoculating harvested seeds in a 1 / 2MS liquid
nutrient medium for nonsymbiotic
germination cultivation, transferring protocorm of seeds in a proembryo stage into a 1 / 2MS liquid
nutrient medium containing 0.01% of colchicin for
mutagenesis cultivation, then transferring into 1 / 2MS
solid nutrient medium for cultivation till seedlings ofdendrobium nobile are formed, carrying out flowering cultivation on tetraplont seedlings with
chromosome numbers of 2n=76, taking tetraplont as a female parent and diploid as a male parent or taking diploid as a female parent and tetraplont as a male parent for hybridization to obtain the triploid; transferring triploid hybridized seeds in a formation early state into the 1 / 2MS liquid nutrient medium containing 0.01% of colchicin for carrying out artificial
mutation hexaploid treatment for 150-180h, and then transferring into the 1 / 2MS
solid nutrient medium till the seedlings are formed, selecting seedlings with
chromosome numbers of 2n=114 as tetraplont
dendrobium nobile for carrying out
bottle seedling transplanting, and finally cultivating and managing according to a conventional method till flowering. The invention lays the foundation for the popularization of new varieties of the
dendrobium nobile.