78 results about "Subfamily" patented technology

The browsable database can allow for high-throughput analysis of protein sequences. One helpful feature may be a simplified ontology of protein function, which allows browsing of the database by biological functions. Biologist curators may have associated the ontology terms with Hidden Markov Models (HMMs), rather than individual sequences, so that they can be applied to additional sequences. To ensure accurate functional classification, HMMs may be constructed not only for families, but for curator-defined subfamilies, whenever family members have divergent functions or nomenclature. Multiple sequence alignments and phylogenetic trees, including curator-assigned information, can be available for each family. Various versions of the browsable database may include training sequences from all organisms in the GenBank non-redundant protein database, and the HMMs can be used to classify gene products across the entire genomes of human, and Drosophila melanogaster.

Compositions and methods for the assessment of T cell receptor variable subunits. The present invention provides nucleotide sequences for the evaluation of the expression of TCRV families. These nucleotides sequences were obtained through a bioinformatic investigation of the nucleotide sequences for TCRVα and TCRVβ families. The nucleotide sequences of the present invention uniquely recognize each and every subfamily and allelic member of a particular TCRV family, while at the same time not recognizing the members of any other TCRV family. This unique expression recognition profile of the nucleotide sequences of the present invention provides great utility for the assessment of TCR families in a clinical setting, such as through polymerase chain reactions, gene chip technology, and direct electrophoretic measurement of DNA or RNA.

A method of producing form-specific anti-peptide antibodies for a wild type protein and its one amino acid mutated protein using a peptide antigen, by obtaining a protein sequence of the wild type protein and its one amino acid mutated protein, selecting a continuous amino acid sequence without any internal cysteine residues that includes the one amino acid mutated sequence and wild type sequence corresponding to the mutated site at the end of the sequence to obtain a synthetic mutation peptide and a synthetic wild type peptide, conjugating the synthetic peptides to a carrier protein, and immunizing an animal to produce antibodies. Methods of detecting cancer and methods of treating cancer.

The present invention relates to novel proteins, termed INSP081, INSP082, and INSP091, herein identified as members of the Germinal Center Kinase (GCK) subfamily of the STE20 family of protein kinases, preferably as NCK-interacting kinases (NIKs) and more preferably as NIK-like embryo specific kinases (NESKs), and to the use of these and nucleic acid sequences from the encoding genes in the diagnosis, prevention, and treatment of disease.

A distant hydridization method between the subfamilies of cyprinid includes chealth eugamic male and female parents catalyzing female, artificial fertilization, incubation and naturalizing to obtain to descendant with strong bionergy. Its advantages are high disease resistance, high survival rate and high growth speed.

The present invention relates to a novel class of AKR1C3 inhibitors, to compositions containing them, to methods for their preparation, and to methods of use thereof. The AKR1C3 inhibitors may be useful in the treatment of, for example, prostate cancer, benign prostate hyperplasia (BPH), lung cancer, acne, seborrhea, hirsuitism, baldness, alopecia, precocious puberty, adrenal hypertrophy, polycystic ovary syndrome, breast cancer, uterine cancer, uterine fibroids, endometriosis, myeloma and leiomyoma.

The invention provides a method for detecting sheep-derived ingredients in glue type traditional Chinese medicines and products thereof. The method provided by the invention comprises the step of detecting by using the difference of a specific nucleotide sequence or amino acid sequence in a genome of a sheep subfamily animal, wherein the specific amino acid sequence is shown in SEQ. ID No1. By adopting the method provided by the invention, whether the glue type traditional Chinese medicines contain the sheep-derived ingredients or not can be detected rapidly, so as to distinguish the authenticity; although polypeptides of collagen in the glue type traditional Chinese medicines are subjected to certain hydrolysis and destruction, the sheep-derived ingredients in the glue type traditional Chinese medicines and products thereof can be identified on the basis that differential polypeptides in a main ingredient, namely collagen, of the glue type traditional Chinese medicine are high in content and little in damaged extent.

A method for distant hybridization between subfamilies of cyprinus carpio and megalobrama amblycephala includes the following steps that firstly, female cyprinus carpio and male megalobrama amblycephala which have obvious sexual maturity characteristics and good somatic features are selected as parent fishes for special pool breeding, then, artificial spawning induction is conducted on the parent finishes in a breeding season, the parent fishes with a good spawning induction effect are selected to be subjected to artificial dry-method fertilization, running water incubation is conducted on fertilized embryos in a pure water incubation device, fish fry are raised after being incubated, the raised fish fry are detected and screened, hybrid offspring of the cyprinus carpio and the megalobrama amblycephala are obtained through flow cytometry DNA content determination and renal tissue chromosome ploidy detection, and the hybrid offspring comprise diploid natural gynogenesis cyprinus carpio, diploid natural gynogenesis mirror carps, diploid fish like the cyprinus carpio and tetraploid hybrid fish. The method has the advantages that the rate of fertilization is high, the incubating rate is high, the offspring are excellent in character, and the method has great significance in genetic breeding.

The invention discloses a DNA extraction method of Duck Enteritis Virus genome and the genome sequence. Firstly, the pyrolysis of chicken embryo fibroblast which is infected with DEV is processed by hypertonic buffer; then the nuclease is added for the digestion of cell DNA; and the virus genome DNA is obtained through phenol chloroform extracting method. The obtained sample is broken randomly into 2kb to 2.5kb fragments; the pUC19 vector is linked to transform host E. coli and construct genomic library. The positive clones in the library are randomly sequenced; the total sequence length has 6 times coverage rate for the virus genome. The sequence result is assembled and clustered to obtain the whole DEV genome sequence with the length of156512bp. The analysis shows that the genome structure is the same with that of the members of Varicella virus in Alpha- herpesus subfamily, which provides theoretical basis for the classification of DEV.

The invention discloses a method for the distant hybridization between subfamilies of red crucian carps and xenocypri davidi bleekers, comprising the following steps: firstly, selecting red crucian carps and xenocypri davidi bleekers with maturated gonads and good body features to be respectively used as female parent fishes and male parent fishes for hybridization, and carrying out artificial induced spawning on the female and male parent fishes in a breeding season; secondly, selecting the male and the female parent fishes with good effect of induced spawning for carrying out artificial dry-method insemination, and carrying out automatic hatching in running water after the insemination is finished; thirdly, breeding hatched fries, and detecting and screening the bred fries to obtain redcrucian carp and xenocypri davidi bleeker hybridized triploid fishes, red crucian carp and xenocypri davidi bleeker hybridized tetraploid fishes and natural gynogenesis red crucian carps. In the invention, the characteristics of the bred hybridized generation are improved, and the insemination rate and the hatching rate of distant hybridization are remarkably improved; in addition, the method is anew way for generating the tetraploid fishes and the natural gynogenesis red crucian carps, and has important biological significance in the aspects of the biological evolution and the genetic breeding of fishes.

The invention discloses a subfamily distant hybridization for German mirror carp and megalobrama amblycephala. The method comprises the following steps of artificial dry-process fertilization on the ova of the pubescent German mirror carp and sperms of the megalobrama amblycephala by taking the German mirror carp as a female parent and the megalobrama amblycephala as a male parent, hatching fertilized ova at the water temperature of 19-20 DEG C, and breeding fries in a pond; then, detecting the DNA (deoxyribonucleic acid) content, the chromosome number and the blood cell sizes of the fries by a flow cytometry DNA content determination method, a chromosome ploidy detection method of peripheral blood cell culture and a blood smear method, screening, and then preparing to obtain triploid hybrid fishes and tetraploid hybrid fishes. A tetraploid fish strain which is genetically stable and has four sets of crucian carp chromosomes can be formed by subsequent self-cross selective breeding of the tetraploid hybrid fishes, and the tetraploid fish strain can be used as a resource for breeding the infertile triploid fishes. The method disclosed by the invention has the advantages that not only are the characters of hybrid offspring good but also a good foundation can be laid for variety breeding, and a precious resource is provided for subsequent breeding of new polyploid fishes.

The present invention relates to novel engineered Ga proteins and assay methods of using such proteins to advance drug discovery. Engineered Ga proteins described by the invention contain alterations of at least one and preferably two or more amino acid residues that are highly conserved among all four subfamilies of Ga proteins. A preferred engineered protein disclosed here is a double mutant, Gαπ R178M A326S. This specific combination of mutations yields an unexpectedly amplified effect on Ga function both in terms of GTPase activity (GTP hydrolysis) and GDP dissociation. This synergistic effect may have a profound influence on the way GPCR signaling pathways are examined for the development of new pharmacotherapies, particularly in the field of central nervous system disorders such as Parkinson's disease.

The invention discloses application of SBP-box type transcription factors of switchgrass in the aspect of increasing plant biomass and fermentable sugar yields, and belongs to the technical field of plant genetic engineering. The application mainly includes cloning genes of the SBP-box type transcription factors PvSPL1 and 2 of the switchgrass and determining sequences of the SBP-box type transcription factors of the switchgrass; inhibiting the transcription activity of integral subfamilies of the transcription factors PvSPL1 and 2 on the basis of the genes and the sequences by the aid of chimeric repressor silencing technologies (CRES-T) so as to generate transgenic switchgrass plants with increased tiller quantities. The application has the advantages that dry matter biomass of the obtained transgenic switchgrass plants can be increased by 2.0-2.3 times, and the total fermentable sugar yields can be increased by 2.0-2.2 times; the generated transgenic switchgrass energy plants can be integrated in conventional breeding projects, and accordingly precious germplasm resources can be provided for creating novel varieties of high-yield and efficiently degradable energy plants; novel targets for molecular breeding in future can be provided by the authenticated SBP-box type transcription factors PvSPL1 and 2 of the switchgrass, and the SBP-box type transcription factors PvSPL1 and 2 of the switchgrass can be used for designing oriented molecules of commercialized energy plant products.

The present invention provides modified, highly potent bone morphogenetic proteins. In particular, the present invention relates to the observation that BMP-6 and BMP-9 are less susceptible to inhibition by Noggin that are other members of the BMP subfamily of proteins. The present invention features chimeric bone morphogenetic proteins in which the middle portion of BMP-6 or BMP-9 replaces the middle portion of another BMP subfamily protein to cause resistance to inhibition by Noggin or other Noggin-like antagonists. Other embodiments of modified BMPs, compositions and methods of use are also included.

The invention discloses a method for distant hybridization of cyprinuscarpiohaematopterus and megalobramaamblycephala subfamilies. The method comprises the following steps: taking cyprinuscarpiohaematopterus as female parent fish and megalobramaamblycephala as male parent fish, artificially inducing spawning of the female parent fish and the male parent fish in a breeding season, carrying out dry artificial insemination, incubating obtained fertilized roe in running water in an incubation tank to obtain hybrids of the cyprinuscarpiohaematopterus and the megalobramaamblycephala, and testing and screening the hybrids to obtain diploid cyprinuscarpiohaematopterus, diploid common-carp and crucian-carp hybrids and diploid crucian carp. The method for distant hybridization of the cyprinuscarpiohaematopterus and megalobramaamblycephala subfamilies integrates the excellent characters of the cyprinuscarpiohaematopterus and the megalobramaamblycephala, enriches the fish species on the market, lays a favorable foundation for selection and breeding of excellent species, provides precious resources for follow-up selection and breeding of novel polyploid fish and is of great biological significance in biological evolution and genetic breeding of fish.

The invention relates to a method for centralized expanding and detecting the gene region of a genome DNA, which aims to verify the characteristics of the gene area in the genome. The characteristics comprise single nucleotide polymorphism (SNP), point mutuation, sequence insertion / deletion and the levels of dideoxy nucleotide (DNA) methylated CpG sites. With an Alu family, particularly an AluY subfamily common sequence as the main oligonucleotide primer, the method expands the DNA and copies all gene regions which are mostly concentrated in the genome with the oligonucleotide primer inter-alu PCR expanded genome DNA. The method is characterized in that the inter-Alu PCR can filter some non-gene sequences, so that a novel generation of sequencing technology detects the SNP, the point mutuation, the sequence insertion / deletion and the levels of DNA methylated CpG sites of the gene region in a centralized way, so as to save the consumption of the genome DNA required by the sequencing.

The invention relates to a kit for testing clozapine application effect based on rs2032582 and an application method of the kit, and particularly relates to a method for testing single nucleotide polymorphism of ABCB1 (ATP binding cassette subfamily B member 1) NO.8 exon in the field of genes. Sequence of the nucleotide, which is isolated and comprises single nucleotide polymorphism locus, is as shown as SEQ ID NO:1; the single nucleotide polymorphism locus is located at NO.3169, rs2032582 (C->T). The method for testing the nucleotide includes the steps of firstly, testing the nucleotide at the locus NO.3169 of the sequence as shown as SEQ ID NO:1; secondly, testing whether there is of the single nucleotide polymorphism at the locus NO.3169 or not by means of a Taqman-MGB typing method. According to the kit, the application method thereof and the method for testing the nucleotide, a foundation for research on ABCB1 gene polymorphism and clinical medicine safety is laid, a theoretical basis for individualization of clinical medicine is provided, and a guidance for research and development of novel medicines based on pharmacogenomics idea is also provided.

The present invention aims at providing a medicament and a method for promoting replication of hepatocytes and a medicament and a method for ameliorating insulin resistance. An effective amount of a neutralizing agent for CXCL10 belonging to a subfamily of chemokines which are heparin-binding proteins is administered to the hepatocytes to promote the replication of the hepatocytes. As the neutralizing agent for CXCL10, a factor which is specifically bound to CXCL10 and inhibits an activity of CXCL10 or a factor which inhibits CXCL10 expression is suitably used. By administering the neutralizing agent to impaired hepatic tissue, it is possible to restore and regenerate the hepatic tissue. Meanwhile, by administering an effective amount of the neutralizing agent for CXCL10 to the hepatocytes, the insulin resistance in type II diabetes and metabolic syndrome is ameliorated.

Found in various eukaryotic organisms, polo-like kinases (collectively, Plks) are a conserved subfamily of Ser / Thr protein kinases that play critical roles in cell proliferation. Provided herein are compounds that specifically inhibit the activity of Plks, specifically Plk1. Further provided herein are methods for use of the compounds for the treatment of hyperproliferative disorders, particularly cancer. Also provided are uses of the compounds for the preparation of a medicament.

The invention relates to rice dwarf straw leaf roll mutant (cd1) gene having the nucleotide sequence SEQ ID No.1, the nucleotide sequence of the corresponding wild type OsCD1 gene is SEQ ID No.2. In the invention, a dwarf straw leaf roll mutant is discovered in an established insert mutant database, the gene (cd1) thereof discloses that the mutant character is controlled by CSLD subfamily genes. In the research, the character shown by the mutant seriously influences the growth and development of rice, the shape construction of the plant is suppressed, the chlorophyll content is lowered, the ripening rate decreases greatly, the seeds are not full, and evolution of agronomic trait is limited; therefore, the inventive rice dwarf straw leaf roll mutant (cd1) gene provides reliable theoretical basis for drought combat, disease resistance and lodging resistance, particularly the invention can change plant shape by using gene engineering method, regulate and control the shape of plant, and has important utilization value in gardening ornamental plants.

Imipridones selectively modulate Class A G protein-coupled receptors (GPCRs), such as the D2-like subfamily of dopamine receptors, and are useful for treating conditions and disorders in need of such modulation, such as cancers. Specifically, the cancer is a midline glioma, a cancer having a histone H3 mutation, or both. In addition, methods of identifying whether a subject having these conditions, is likely to be responsive to a treatment regimen, such as imipridone administration, are provided. Furthermore, methods of assessing the effectiveness of a treatment regimen, such as imipridone administration, monitoring, or providing a prognosis for a subject with these condition are also provided.

The present invention relates to novel engineered Ga proteins and assay methods of using such proteins to advance drug discovery. Engineered Ga proteins described by the invention contain alterations of at least one and preferably two or more amino acid residues that are highly conserved among all four subfamilies of Ga proteins. A preferred engineered protein disclosed here is a double mutant, Gαπ R178M A326S. This specific combination of mutations yields an unexpectedly amplified effect on Ga function both in terms of GTPase activity (GTP hydrolysis) and GDP dissociation. This synergistic effect may have a profound influence on the way GPCR signaling pathways are examined for the development of new pharmacotherapies, particularly in the field of central nervous system disorders such as Parkinson's disease.

The invention provides a rubber tree HbAG gene, having a nucleotide sequence as shown in SEQ ID NO: 1. The invention further provides a protein coded with the HbAG gene and application of the HbAG gene. The HbAG gene is cloned from a rubber tree for the first time, and the gene belongs to AG subfamily, participates in development of flowers, formation of ovules and maturation of seeds and executes the functions of D genes. Subcellular localization analysis shows that the gene is localized in a cell nucleus and thus conforms to the feature of a transcription factor. The gene is further transferred to arabidopsis to obtain a transgenic plant, and the transgenic plant is discovered to have such phenotypes that the flowering time is obviously advanced, the nutritional growth time is short, and the plant is dwarfed and the like, so the gene plays an important control role in flower organ development and reproductive development of rubber trees. By using the HbAG gene for genetic improvement on rubber trees, an effective gene resource can be provided for shortening the breeding periods of the rubber trees, improving the breeding efficiency and the like.

The invention relates to compounds acting as selective antagonists of Transient Receptor Potential cation channel subfamily M member 8 (TRPM8), and having formula (I).Said compounds are useful in the treatment of diseases associated with activity of TRPM8 such as pain, inflammation, ischaemia, neurodegeneration, stroke, psychiatric disorders, itch, irritable bowel diseases, cold induced and / or exacerbated respiratory disorders and urological disorders.

TRPC ion channels are non-selective channels widely expressed in human tissues. These channels are involved in numerous physiological functions and are putative targets for the development of novel medicines. There is a need to gain a better understanding of TRPC ion channels in cells and beyond. The present invention provides a pharmacological tool compound that allows to study TRPC ion channels due to its discriminating inhibition of TRPC subfamilies. In the present invention, norgestimate is shown to selectively inhibit TRPC3, TRPC6 and TRPC7.

The invention relates to a chrysanthemum photoperiod-sensitive gene CmFT and application of the CmFT gene in chrysanthemum photoperiod sensitivity. In the invention, the CmFT, an FT homologous gene, is separated out from chrysanthemums by using a homologous gene cloning method as well as a RACE technology, the gene has a nucleotide sequence as shown in SEQ ID No.1, and the CmFT belongs to FT subfamily members. The CmFT is relevant to chrysanthemum photosensitivity. After transforming the gene into model plant Arabidopsis thaliana and common chrysanthemum varieties by genetic transformation, the result shows that the gene can adjust the photoperiod of chrysanthemums and promote flowering.