687 results about "RNA extraction" patented technology

The invention relates to the field of molecular biology and discloses a method and a kit for extracting ribonucleic acid (RNA). The method for extracting the RNA comprises the following steps of: uniformly mixing a sample and lysis solution; centrifugally collecting supernate and uniformly mixing the supernate and absolute ethyl alcohol which is half of the volume of the supernate; combining the mixture with 0.45 mu m glass fiber cellulose acetate membrane; washing with deproteinization liquid and rinsing liquid; eluting adsorbed RNA; uniformly mixing lysis cells and an extraction aid; and centrifugally removing polysaccharide and polyphenol substances. The kit for extracting the RNA comprises the lysis solution, the deproteinization liquid, the rinsing liquid, the eluting liquid and the extraction aid. The method of the invention has the advantages of simpleness, rapidness, no use of toxic reagent, wide application range and capabilities of effectively removing the polysaccharide andpolyphenol substances and separating high-quality RNA out by adding the extraction aid. The kit of the invention has the advantages of no toxic reagent, wide application range, RNA extraction effectsof plant materials which are rich in polysaccharide and polyphenol superior to that of foreign kits, low cost and suitability for extensive laboratories and scientific researches.

The present invention provides methods and compositions for extracting RNA from cells. The cellular extract may be directly used in a variety of reactions, such as reverse transcription and PCR.

The invention provides a detection method of Chikungunya virus, which comprises the procedures as follows: pretreatment of test sample; RNA extraction of test sample to be detected; design and synthesis of primer and probe; determination of optimum fluorescence quantitative PCR reaction system; program amplification and adjudging results according to Ct value. The optimum fluorescence quantitative PCR reaction system is determined as follows: the CHIK-FP terminal concentration of positive primer is 500nM, the CHIK-RP terminal concentration of reverse primer is 1000nM, and the CHIK-Probe terminal concentration of probe is 250nM. The amplification program is 45 times of circulation of 10 minutes at 50 DEG C, 10 minutes at 95 DEG C, 10 seconds at 95 DEG C and 30 seconds at 62 DEG C. The invention establishes a detection method for Chikungunya virus, which can be applied on monitoring of the virus, and can further strengthen the monitoring work for preventing from spreading the disease into our country and has important social efficiency and economic efficiency.

The invention provides a method for extracting DNA and RNA of a plant synchronously, which utilizes one plant sample; and after the plant sample is treated and decontaminated with the same steps, the DNA and RNA thereof are settled and purified respectively, thereby achieving the purpose of extracting synchronously. The method can be applied to the synchronous extraction of DNA and RNA of a yam sample and has the advantages of being able to synchronously conduct nucleic acid extraction, time-saving, economical and fast; besides, the ultracentrifugation, KAC and the like adopted by the method are very effective in eliminating the impurities contained in the yam such as amylose and the like; the DNA / RNA extraction quality of the method is high; the RNA OD260 / 280 ratio is between (1.8 to 2.0) and the DNA is between 1.7 to 1.8; and the electrophoretic band is complete and clear. The method is also applicable to the nucleic acid extraction of other plant samples.

The invention discloses a primer and a probe combination for detecting bovine viral diarrhea virus by a recombinase polymerase application (RPA) technology. The forward primer sequence is as shown in SEQ ID No.1; the reverse primer sequence is as shown in SEQ ID No.2; and the probe sequence is as shown in SEQ ID No.3. The invention also discloses a kit for bovine viral diarrhea virus detection. When the primer and the probe are used for detecting, a clinical sample only needs the treatment steps of virus RNA extraction, reverse transcription by a one-step method to form cDNA, and isothermal amplification, thermal cycle reaction is not needed, amplification does not need to be performed in a polymerase chain reaction (PCR) instrument, and the result can be displayed clearly on a lateral flow assay test strip, so that the primer and the probe have the advantages of high sensitivity, high specificity, simple reaction procedure, short detection time and the like, and are applicable to quick, accurate, simple and convenient diagnosis and treatment on the diseases in cattle farms.

The present invention provides RNA extraction reagents, methods and kits that are especially useful for extracting RNA. The reagents, methods and kits of the present invention are especially useful for extracting RNA, for example, cytoplasmic RNA, from difficult materials, from plants, especially, difficult plant tissues, such as those containing phenolics, tannins, polysaccharides (such as starch) and resins. Comparative high yields are obtainable according to the present invention when compared to conventional reagents and methods. The RNA preparations obtained in accordance with the present invention are also of high quality as demonstrated by superior A260 / 280 results and by gel electrophoresis.