The invention discloses a method for preparing a micro-fluidic
chip for culturing and detecting
lung cancer cells. The method comprises the following steps: preparing a
mask; preparing a hydrogel material; preparing a
cell culture layer; arranging the
mask of the
cell culture layer on a glass substrate, and injecting the hydrogel material under shading conditions, so that the hydrogel material is cured; preparing a micro-channel layer, namely arranging the
mask of the micro-channel layer on the prepared
cell culture layer, and injecting the hydrogel material under the shading conditions, so that the hydrogel material is cured; and eluting and preparing a sealing cover layer, namely eluting the prepared micro-channel layer, and sealing by using PDMS. According to the micro-fluidic
chip prepared by the method disclosed by the invention, multiple groups of cell samples can be simultaneously cultured, a three-dimensional growth environment is provided for the cells, the
human lung tissue structure is completely simulated, a growth environment close to the
human body is provided for the cells, and the
metabolite is very close to the
metabolite of human cells, so that the detection data is closer to the human real conditions, and early diagnosis of
lung cancer is promoted.