The invention discloses a micro-fluidic
chip which comprises a substrate, a sampling part and a bottom cover, wherein a sample feeding cavity, a pre-treating region, a detection cavity and a reaction region are formed in the substrate; the sampling part comprises a sample feed inlet, a sample accommodating cavity and a sample outlet; the pre-treating region comprises a pre-treating
reagent cavity, a pre-treating cavity and a gas cavity; the reaction region comprises a reaction
reagent cavity and a reaction cavity; elastic cavity walls are formed on the pre-treating
reagent cavity, the gas cavity, the reaction reagent cavity and the reaction cavity; a transparent panel is arranged on the upper surface of the detection cavity and a transparent bottom plate is arranged on the lower surface of the detection cavity; and the transparent panel and the transparent bottom plate correspond to each other. Based on a transmitting
turbidimetry, according to the micro-fluidic
chip, the content of a to-be-detected component in a sample is quickly and quantitatively detected by means of a micro-fluidic
chip technology, so that the micro-fluidic chip has the characteristics of being easy to operate, high in sensitivity and accurate in result. A preparation method of the micro-fluidic chip comprises the steps of preparing a reagent; and assembling the chip.