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Retinol conjugated protein detection kit

A detection kit and protein-binding technology, which is applied in the field of medical immunity, can solve the problems of reagent quality degradation, easy coalescence, poor reagent stability, etc., and achieve the effect of simple detection, high sensitivity and low production cost

Inactive Publication Date: 2016-04-13
宁波天康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the connection between antibody and latex is usually carried out by chemical coupling method. As an R2 reagent, it is very easy to coalesce, which makes the quality of the reagent decrease with the prolongation of storage time.
Poor reagent stability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0037] The determination of embodiment 2 retinol binding protein

[0038] Detection tool: Hitachi 7060 automatic analyzer.

[0039] Analysis method: two-point endpoint method; main wavelength: 570nm, secondary wavelength: -: sample volume: 2ul (microliter); R1: 200ul; R2: 50ul; reaction direction: rising; measurement temperature: 37°C; sample mixed with R1 After mixing, read the absorbance A1 at 10 seconds; add R2 at 3-5 minutes, and read the absorbance A2 after 5 minutes. The reaction absorbance was calculated as the difference between A2 and A1.

[0040] Calculation method: multi-point calibration, the dose / response curve is made according to the absorbance and reference serum value, and the sample content can be calculated on the dose / response curve according to its absorbance value.

Embodiment 3

[0041] Example 3 Retinol-binding protein detection kit performance evaluation

[0042] 1. Analytical Sensitivity Assessment

[0043] Use 5% bovine serum albumin solution as a blank sample, and the blank sample should not contain the analyte. 20 consecutive detections were performed on the biochemical analyzer, and the mean and standard deviation SD of the 20 results were calculated. The detection limit of the reporting method is the mean value of the blank plus two standard deviations (+2SD). As can be seen from Table 1, the sensitivity is 2.68mg / L.

[0044] Table 1

[0045] 2. High value linear evaluation

[0046] 1 part of low-value serum (10mg / L) and 1 part of high-value serum (100mg / L) according to the ratio of 9:1, 4:1, 2:1, 1:2, 1:4, 1:9 (sample sequence number 1 to 6), prepared 6 samples with different concentrations, each sample was measured twice, as can be seen from Table 2,

[0047] The highest detection range of the detection kit of the present invention ca...

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Abstract

The invention discloses a retinol conjugated protein detection kit. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is a buffer solution, the reagent R2 is the mixture of retinol conjugated protein antibody sensitized polystyrene latex particles and the buffer solution. The retinol conjugated protein detection kit has the advantages that the kit is simple and quick to detect, high in sensitivity, good in accuracy, good in anti-interference capacity and low in production cost; a lipoprotein a (retinol conjugated protein) detection method adopted by the kit is a latex enhanced immuno-turbidimetry and enables the lipoprotein a (retinol conjugated protein) detection to be more economical, convenient and quicker, and the retinol conjugated protein detection kit is applicable to automatic biochemical analyzers of most of hospitals and especially can achieve quick quantitative detection for emergency treatment.

Description

technical field [0001] The invention belongs to the field of medical immunity, and relates to an immunological detection reagent. Furthermore, the invention relates to a retinol-binding protein detection kit and a detection kit. Background technique [0002] Retinol-binding protein (RBP) assay kit, this product is used for in vitro quantitative determination of the content of retinol-binding protein in human serum for auxiliary diagnosis. RBP is mainly synthesized in the liver, so decreases and increases in serum RBP are associated with and influenced by the presence and severity of liver disease. In liver disease, liver cirrhosis and acute and chronic hepatitis, serum RBP levels were significantly reduced. [0003] RBP can be used as an early diagnostic indicator of renal tubular injury. Retinol-binding protein RBP has strong stability in urine, is not easy to decompose, and is not disturbed by pH and blood pressure. When the renal proximal tubule is injured, the urine o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/531
CPCG01N33/68G01N33/531G01N33/577
Inventor 陆雪龙宋高峰李清华周裕国
Owner 宁波天康生物科技有限公司
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