309results about How to "Rapid Quantitative Detection" patented technology

The invention belongs to the technical field of micro fluid control, particularly disclosing a micro fluid control chip and a preparation method and application thereof. One side of the chip is provided with a plurality of sample introduction holes, each sample introduction hole is communicated with a sample introduction runner; after meeting, each sample introduction runner is communicated with a main runner; the main runner is provided with a narrow runner; a micro-sphere of which the size is 0.5-1.5 mm is filled in front of the inlet of the narrow runner; the outlet of the main runner is communicated to a waste liquid tank arranged at the other side of the chip. The preparation method mainly comprises: firstly preparing a PDMS substrate; then, preparing a PDMS cover plate with an upper groove; sticking the substrate onto the cover plate to obtain a semi-finished product of the chip; carrying out modified treatment on each runner in the semi-finished product of the chip; finally, putting the micro-sphere in, controlling the charge quantity, and finishing production. The micro fluid control chip of the invention has high solution mixing efficiency, stable solution flow rate, small volume, convenient carrying and relatively simple manufacturing; when being used for detecting the density of triphosadenine, the micro fluid control chip has the advantages of high sensitivity, favourable detection effect and the like and can not be affected by environment humidity.

The invention in particular relates to a whole-blood labeled immunoassay method for quickly collecting a target object from whole-blood through specificity binding reaction and labeling and monitoring the target object by a labeling technology, and an instant detection system. The method comprises the following steps: 1) capturing magnetic beads; 2) eluting blood; 3) labeling a detection antibody; 4) eluting the detection antibody; 5) detecting. The system comprises a microfluidic chip, a magnetic field generation device arranged above or below the microfluidic chip and a detection device, wherein the microfluidic chip comprises a microchannel, the magnetic beads arranged in the microchannel, a waste liquid outlet and an eluent storage pond; the microchannel is separated into a first reaction region, a second reaction region and a detection region in stages by a separation mechanism. The technical scheme provided by the invention has the characteristics of high sensitivity, high repeatability, simple system structure, low cost and the like, and can detect the target object from trace blood samples quickly and quantitatively.

The invention discloses a fluorescent microsphere immunochromatographic testing card for testing five indexes of hepatitis b and a method for preparing the same. The testing card comprises a hepatitis b surface antigen test paper strip, a hepatitis b e surface antigen test paper strip, a hepatitis b surface antibody test paper strip, a hepatitis b e surface antibody test paper strip, and a hepatitis b core antibody test paper strip. Each test paper strip is formed by overlapping and bonding filter paper, a sample pad, a glass fiber film spray-coated with fluorescent microspheres, a cellulose nitrate film and water absorption paper on a bottom plate by glue in sequence, wherein the cellulose nitrate film is coated with antigens serving as a testing area and anti-rabbit antibodies serving as a quality control area; and during a test, after emitted fluorescent light passes a filter, the emitted spectrum is collected, accumulated and multiplied by the CCD scanning technology and then converted into a numerical signal, the numerical signal is multiplied by a correction factor, and the strength of the corrected fluorescent light is substituted in a standard curve of a fluorescence analyzer, so that the concentrations of the five indexes of hepatitis b of the sample can be automatically worked out. The test of hepatitis b viruses by the testing card has the characteristics of specificity, sensitivity, simpleness and accuracy.

The invention relates to an immunochromatography time resolution fluorescence kit for synchronously detecting mixed pollution of five types of fungaltoxinfungi toxins of aflatoxin and the like and application. The immunochromatography time resolution fluorescence kit comprises an immunochromatography time resolution fluorescence test paper strip and sample reaction bottles of monoclonal antibody freeze-drying samples containing europium labels, wherein the fluorescence test paper strip comprises a PVC (polyvinyl chloride) substrate; a water absorbing pad, a detection pad and a sample pad are sequentially adhered onto one surface of the substrate; the adjacent pads are overlapped and connected at the connecting part; the detection pad uses a nitrocellulose membrane as a base pad, and is provided with a transverse quality control line and five detection lines from top to bottom to respectively coat the bovine serum albuminaflatoxin B1-BSA conjugate of each toxin; a fumonisin B1 monoclonal antibody is excreted by a hybrid tumor cell strain Fm7A11 with collection number of CCTCC NO.C201636. The immunochromatography time resolution fluorescence kit is applied to synchronously detect the mixed pollution of toxins of aflatoxin B1, fumonisin B1, ochratoxin A, zearalenone and aspergillus versicolor, and has the advantages that the operation is simple and quick, and the sensitivity is high.

The invention discloses a method for detecting miRNA (ribonucleic acid). The method comprises the steps: (1) synthesizing a DNA (deoxyribonucleic acid) tetrahedron probe; (2) connecting three peak points of the DNA tetrahedron probe to the surface of a working electrode of an electrochemical device to obtain a working electrode with a capture probe; (3) adding a target miRNAs to be detected, a signal probe and an auxiliary chain into a reaction system to implement hybridization reaction to form a composite body I, and immersing the working electrode into the reaction system to implement hybridization reaction to form a composite body II; (4) generating reaction between the composite body II and enzyme capable of generating catalytic oxidation reduction reaction; (5) adding a primer produced by the enzyme catalytic oxidation to realize electrochemical detection analysis. The target miRNAs can be directly detected by the method disclosed by the invention without being marked and subjected to pre-PCR (polymerase chain reaction) multiplication; the method is easy to operate, so that the experiment cost is greatly reduced, and the experiment efficiency is improved.

The invention relates to preparation and application of a paper-based microfluidic chip for colorimetric analysis; the preparation comprises the step of enabling a hydrophilic channel to be jointed on a hydrophobic substrate, wherein the hydrophilic channel is obtained by printing the pattern of the channel on a piece of filter paper and then shearing the pattern, and the hydrophobic substrate is obtained by treating the filter paper with a normal hexane solution containing methyl trichlorosilane and octadecyl trichlorosilane; the paper-based microfluidic chip is combined with a colorimetric analysis method, so that rapid quantitative detection can be realized. The preparation method is simple and fast, short in production cycle, low in cost, low in energy consumption and free from pollution. The preparation and the application of the paper-based microfluidic chip for colorimetric analysis are suitable for the fields such as clinical diagnosis, food hygiene, environmental monitoring and biochemistry, thus having very wide application scope.

A test strip detection system, comprising a test strip card (1) and a detection device (2); the test strip card (1) comprises a card box (16), a built-in test strip (15) and an electronic label (20) matched with the built-in test strip (15); the electronic label (20) stores parameters such as the standard working curve of an object to be detected and the like; the detection device (2) comprises an optical system (3), a photoelectric detector (4), an analog / digital converter (5), a data processing device (6), an electronic label read-write module (10) with an aerial (11), a voice module (34), a cell box (7) and an output display device (8). The system further comprises a wireless communication module (12) and a wireless network system (13) connected with the wireless communication module (12) and comprising a remote server (14). The data processing device (6) calculates a sample detection result according to the characteristic frequency optical signals transmitted by a test strip detection band (27) and a quality control band (28) in combination with an electronic label (20) transmission parameter; the detection result is displayed on the output display device (8); the voice module (34) vocally prompts the detection result at the same time; the detection result is transmitted to the remote server (14) via the wireless communication module (12) for data management and information feedback.

The invention discloses a preparation method for a paper-based micro-fluidic chip. The trimethoxy silane is processed in a heptane solution to obtain a hydrophobic base. Meanwhile, patterns printed on the channel of a piece of filter paper are cut, and then are immersed in cetyl trimethyl ammonium bromide to obtain a hydrophilic base. The above hydrophobic base and the above hydrophilic base are bonded together to form a hydrophilic and hydrophobic access. Compared with the traditional method, the above preparation method is free of any expensive instrument / agent / metal mold, thus being novel, simple, quick, low in cost and short in production period. The entire preparation process can be completed within 7 minutes.

The invention discloses a novel SERS substrate-based method for quantitatively testing pathogenic bacteria, and belongs to the technical field of biological detection. Silicon dioxide treated by an immunospectral marker is coated with gold nanoparticles for quantitative test of the pathogenic bacteria. Raman spectra signal monitoring data are more stable and reliable and can be greatly repeated. The method is good in test stability, high in sensitivity, high in specificity, the detection process is simple, fast, accurate and quantitative, and a wide detection interval and a low detection limit are obtained. The method is simple in operation, an SERS immune signal probe and a capture probe can be prepared in advance, the capture probe and the signal probe only need to be sequentially added to a to-be-detected sample for reaction during operation and the test is carried out at once through magnetic separation after the reaction is completed: the collection time is 30s, and then the concentration is immediately read from a standard curve, thereby achieving fast quantitatively testing. The method can meet the requirements of food safety and environment monitoring departments, and has wide practicability.

The invention discloses a novel fluorescence immunochromatography test strip for joint inspection of SARS-CoV-2 IgG-IgM antibodies of coronaviruses. The test strip comprises a bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, wherein the sample pad, the combination pad, the nitrocellulose membrane and the water absorption pad are sequentially connected end to end and adhered to the bottom plate; the combination pad is coated with an SARS-CoV-2 structural protein-marker and a goat anti-rabbit IgG-marker, and the nitrocellulose membrane is provided with a detection line T1 coated with a mouse anti-human IgG monoclonal antibody, a detection line T2 coated with a mouse anti-human IgM monoclonal antibody and a quality control line C coated withrabbit IgG. When the test strip is used for quantitatively detecting SARS-CoV-2 IgG and IgM antibodies, the detection sensitivity is high, and the specificity is good and can reach 96%; the batch-to-batch difference is small, and good repeatability is achieved; the test strip can be stored for half a year at normal temperature without reducing the sensitivity and has good stability; the test strip is simple to operate and low in cost, can quickly and quantitatively detect the levels of SARS-CoV-2 IgG and IgM antibodies in a human body, assists a nucleic acid detection means, and provides powerful support for epidemic situations.

The invention discloses a method for synchronously detecting a plurality of organophosphorus fire retardants in bottom mud, belonging to the detection field of trace amount of organophosphorus fire retardants in the environment. The method comprises the main steps of firstly, extracting a target object in a sample through an accelerated solvent extraction instrument; purifying by a gel permeation chromatography; further purifying and enriching by adopting a solid-phase small extraction column; and finally, concentrating and making up to the constant volume and detecting and quantifying by using a gas chromatograph-mass spectrometer. According to the invention, a pre-treatment method for the organophosphorus fire retardants in the bottom mud is established and optimized, the automation degree is high and the repeatability is good; the gas chromatograph-mass spectrometer is used for carrying out quantitative detection, the detection limit is low and the sensitivity is high; the detection limit to nine types of the organophosphorus fire retardants are lower than 0.340 microgram per gram. According to the method, the synchronous analysis and detection on the plurality of trace amount of organophosphorus fire retardants in a complicated environment medium, namely the bottom mud, is realized; the sensitivity and the accuracy are achieved, and the method makes up the disadvantages of the technology in the field.

The invention provides a Raman spectrum based detection method for oxidative rancidity of ganoderma lucidum spores oil. Acid value and peroxidation value of the ganoderma lucidum spores oil are taken as evaluation indexes of the oxidative rancidity degree of the ganoderma lucidum spores oil. The detection method includes taking Raman peak integral strength of 1655cm-1 in the Raman spectrum of a standard sample as a vertical coordinate and taking the acid value or the peroxidation value as a horizontal coordinate to create standard acid value and peroxidation value evaluation drawings of the ganoderma lucidum spores oil; detecting the Raman spectrum of a tested sample, and comparing a result of the Raman peak integral strength of 1655cm-1 with the standard acid value and peroxidation value evaluation drawings to acquire the acid value and the peroxidation value of the tested sample to further acquire an oxidative rancidity degree evaluation result of the tested sample of the ganoderma lucidum spores oil. The Raman spectrum based detection method has the advantages of short detection time, low sample consumption, simplicity and convenience in operation and on-site quick detection, thereby having a broad practical application prospect.

The invention provides a super-paramagnetic nanoparticle for capturing exosomes and a preparation method thereof as well as a specific exosome luminescence immune quantitative detection kit, and belongs to the technical field of exosome detection. The super-paramagnetic nanoparticle for capturing the exosomes, provided by the invention, comprises a super-paramagnetic nanoparticle base body and anexosome shared marker antibody coupled with the super-paramagnetic nanoparticle base body. The specific exosome luminescence immune quantitative detection kit provided by the invention comprises the super-paramagnetic nanoparticle for capturing the exosomes, an exosome specific marker antibody for luminescence labeling, confining liquid, a washing solution, a NaOH water solution, hydrogen peroxideand a calibrator, and can realize simple, rapid and quantitative detection of the specific exosomes; the specific exosome luminescence immune quantitative detection kit can be directly used for detecting a marker of the specific exosomes in blood serum, blood plasma, pleuroperitoneal fluid, urine and cerebrospinal fluid samples; the specific exosome luminescence immune quantitative detection kithas the advantages of high sensitivity, good stability, short detection time and simplicity in operation and is very applicable to clinical detection.

The invention discloses a magnetic immune chromatography method capable of synchronously detecting Tm and Pa allergens. The method is characterized by comprising the following steps of: sequentially sticking a sample pad, a combination pad combining anti-Tm and anti-Pa immune magnetic beads, a chromatography film and a water absorption pad to a bottom plate crossly at intervals of about 2 millimeters, and then covering a transparent plastic sealing film on the upper layer to construct a magnetic immune chromatography test strip capable of synchronously detecting the Tm and Pa allergens. A Pa antigen detection line T1, a Tm antigen detection line T2 and a goat anti-mouse IgG quality control line C are pre-coated on the chromatography film, the immune magnetic beads can be captured by chromatography, and quick qualitative detection of the Tm and Pa allergens is realized according to 1 to 3 macroscopic color development strips formed after the chromatography of a sample; or quantitative detection of single allergen or synchronous quantitative detection of two allergens is realized by detecting the constructed magnetic immune test strips through a magnetic analyzer according to the magnetic signal detection values of the detection lines and the control line formed after the chromatography of the sample.

The invention relates to a fluorescence probe, a preparation method and an application thereof. The fluorescence probe is arranged as an addition activating type probe, can be used for specific fluorescent mark of protein and also can be used for ration, detection, dynamic and activity study of protein and cell, tissue and in vivo images.

The invention discloses a zearalenone-vomitoxin double-channel immune quantitative test strip, and belongs to the technical field of immunoassay and rapid detection. A fluorescent probe is prepared bymarking fluorescent microspheres; a fluorescent microsphere-zearalenone monoclonal antibody, a fluorescent microsphere-vomitoxin monoclonal antibody and a fluorescent microsphere-goat anti-rabbit secondary antibody are included, and zearalenone artificial antigen, fluorescent microsphere artificial antigen and goat anti-mouse secondary antibody are sprayed on a nitrocellulose membrane to serve asa detection line T1, a detection line T2 and a quality control line C respectively to prepare the immunochromatographic test strip; and zearalenone and vomitoxin in the sample are analzyed simultaneously and quantitatively by reading the fluorescence value of the detection lines in a fluorescence immunoassay analyzer by means of a competitive immunoassay method. The method overcomes the defect that colloidal gold is not easy to store in the test strip technology, and the method for preparing the fluorescent probe is simple, efficient and high in sensitivity.

The invention relates to the technical field of a biosensor, and particularly relates to a fluorescence biosensor for detecting UDG (Uracil-DNA Glycosylase) on the basis of a polymerase-assisted feedback rolling circle amplification and endonuclease amplification fluorescence method. The invention aims to solve problems of both low specificity and low sensitivity of a method for detecting UDG in the prior art. The biosensor for detecting the UDG on the basis of a feedback rolling circle amplification technology achieves a rolling circle amplification effect on matching of phi29 polymerase andendonuclease IV, implements fluorescence resonance energy transfer of a fluorophore and a Quenching group and performs a homogeneous reaction on mixed liquid. A preparation method comprises: constructing a circular template and a composite probe; feeding back a rolling circle amplification signal and carrying out fluorescence detection. Specific hydrolysis of the UDG on a basic group U is utilized, and by utilizing such specific reaction, the UDG can be accurately measured and meanwhile, interference can also be avoided; by utilizing endonuclease IV circle amplification, a signal amplificationeffect is achieved.

The invention discloses a paper chip enzyme-linked immunoassay test card for a combined multi-tumor marker test. The test card is characterized in that upper, middle and lower filter paper chips, a double-sided tape and a paper-based slide frame are arranged between an upper shell and a lower shell; the upper, middle and lower filter paper chips refer to wax patterned filter paper chips which are provided with hydrophilic and hydrophobic regions and are alternately arranged respectively; a sample introduction and liquid feeding window is formed in an upper shell body; the upper filter paper chip is arranged between the upper shell and the double-sided tape; the middle filter paper chip is arranged between the double-sided tape and the lower filter paper chip, and sensitive regions which are distributed in arrays and used for fixing different reaction reagents are arranged on the middle filter paper chip; the water-based slide frame is arranged between the lower filter paper chip and the lower shell; the upper and middle filter paper chips are bonded through the double-sided tape; the lower filter paper chip slides on the water-based slide frame relative to the upper and middle filter paper chips, so that the different reaction reagents can sequentially flow among the multiple layers of filter paper chips. According to the test card, an early diagnosis device applied in clinical site is provided for patients.

The invention discloses a three-carbon electrode electrochemiluminescence base fabric micro-fluidic chip and a preparation method and application thereof. The preparation method of the chip comprises the following steps of designing the shape of a reaction tank and patterns of electrodes, and then obtaining a screen of the reaction tank and a screen of the electrodes, wherein the electrodes are three-carbon electrodes and include the working electrode, the counter electrode and the reference electrode; conducting screen printing of the shape of the reaction tank on a fabric, conducting screen printing of the patterns of the electrodes on the same fabric, and conducting airing to obtain the three-carbon electrode electrochemiluminescence base fabric micro-fluidic chip. The electrode screen printing processing method is applied onto the base fabric micro-fluidic chip for the first time, the processing method has the advantage that no expensive or complex instruments are needed, and has the other important advantage that the base fabric micro-fluidic chips with the reaction tanks and the electrodes can be obtained in batches. The electrodes are the three-carbon electrodes, and compared with a traditional three-electrode system, the manufacturing cost is low; usage for one time can be achieved, and no complex polishing pretreatment is needed.

The invention discloses a method for quickly and quantificationally detecting live bifidobacterium and relates to a quick and quantificational detection method for the live bifidobacterium in products containing bifidobacterium. A detection technology of the bifidobacterium is divided into the prior detection with plate bacterial colony counting as a base and various detections with molecular biology as a base. The method comprises the following steps that: a sample is taken, is diluted up to 10 times, and is subjected to EMA treatment, DNA of the sample is extracted, the molecular beacon-real-time PCR detection is performed, the content of the bifidobacterium is calculated according to a standard curve, wherein, the extraction process of the DNA is that: the sample which is subjected to the EMA treatment is added with 18 percent sodium citrate and 1M NaOH and is centrifugated for 10min at a rotating speed of 10,000rpm, somatic cells are collected and are washed by ultrapure water, a bacterial suspension is taken, is added into a Tritox-100 liquid with a concentration of 2 percent, is subjected to water bath treatment for 10min at a temperature of 100 DEG C, and then is cooled immediately, and supernate is used for molecular beacon-real-time PCR amplification. The method is used for quantitative detection of the live bifidobacterium in the products containing the bifidobacterium.

The invention discloses a beta 2-microglobulin detection kit which comprises a reagent R1 and a reagent R2, and the reagent R1 is a buffer solution; the reagent R2 is a mixture of beta 2-microglobulin antibody sensitization polystyrene latex particles and a buffer solution. The detection kit has the advantages that the detection is simple and rapid, the sensitivity is high, the accuracy is good, the disturbance resistance is high and the production cost is low. An adopted beta 2-microglobulin detection method is latex enhanced immunoturbidimetry, the beta 2-microglobulin detection is more economical, convenient and rapid with the method, and the beta 2-microglobulin detection kit is suitable for automatic biochemistry analyzers in vast majority of hospitals, and particularly suitable for realizing rapid quantitative detection on emergency treatment.

The invention discloses a method for detecting exosome small molecule metabolites by using mass spectrometry. The method comprises the following steps of preparing an instrument and a reagent; preparing an iron-containing oxide nanometer particle substrate; preparing an exosome sample; respectively taking the exosome samples diluted according to different proportions; performing sample application onto a target plate; performing drying at the room temperature; dispensing the iron-containing oxide nanometer particle substrate onto the exosome sample; performing drying at the room temperature; performing mass spectrometric detection; analyzing the mass spectrometric detection result to draw the conclusion. The method has the advantages that the micro-nano particle materials are used as the substrate; the qualitative and quantitative detection on molecules to be detected is fast and efficiently realized by using few biological samples in mass spectrometric analysis; meanwhile, the background interference and hotspot effects of the conventional substrate can be avoided; the detection on metabolism molecules in the exosome samples is realized.

The invention discloses a method of detecting biological molecules by a nano enzyme immune sandwich novel technology. The method comprises the following steps: (1) coupling a first monoclonal antibodyto first magnetic nano enzyme particles to obtain a capture probe; (2) coupling a second monoclonal antibody to second magnetic nano enzyme particles to obtain a detection probe; (3) incubating a to-be-detected sample and the capture probe; (4) obtaining a capture probe-antigen compound from a solution in the step (3); (5) resuspending the capture probe-antigen compound, adding the detection probe, and carrying out incubation; (6) obtaining a capture probe-antigen-detection probe compound from the solution in the step (5); (7) adding a peroxide and a catalytic primer to develop color, and detecting the light absorbance value of the solution through an enzyme-linked instrument; and (8) measuring biological molecules of known concentration and drawing a standard curve to obtain the antigencontent in the to-be-detected biological sample. The method is simple and rapid in operating step, environment-friendly and suitable for detecting various chemical or biological molecules.

The invention relates to a beta 2-acceptor stimulant ELISA kit, which comprises the following compositions in proportion: a coating plate, a calibrator, an enzyme labelling object operating fluid, a substrate liquid A, a substrate liquid B, a stopping solution, a condensed washing liquid and a condensed extract. The kit sensitivity is 0.1 ppb, and the precision is as follows: intra assay variation coefficient (CV%) is 5%, and inter assay variation coefficient (CV%) is 10%; the accuracy is shown by recovery rate, and the recovery rate is in the range of 80-110%. IC 50 range is between 0.3 and 0.5 ppb, and the linear correlation coefficient |r| is not less than 0.9900, and the product has the advantages of wide usage scope, convenient usage and accurate detection.

The invention provides a rapid detection method for the content of aflatoxin in brown rice based on FT-NIR technology. The method comprises the following steps: step 1, sample preparation: a step of collecting brown rice sample with different Aspergillus flavus invasion degrees, crushing the brown rice samples to obtain sample powders and refrigerating the sample powders for detection; step 2, spectral detection: a step of collecting diffuse reflection spectral information of the sample powders by using a Fourier transform near-infrared spectrometer and determining the contents of aflatoxin B1, B2, G1 and G2 and a total amount thereof in the sample powders by using a multifunctional column purification-high performance liquid chromatography-fluorescence method; step 3, spectral pretreatment: a step of pretreating original spectral information of the sample powders obtained in the previous steps so as to eliminate interference; step 4, quantitative forecasting and analysis: a step of analyzing the pretreated spectral information by using a stepwise multivariate linear regression method (SMLR) and establishing a stoichiometrical model of the contents of aflatoxin B1, B2, G1 and G2 and the total amount thereof in the sample powders and the spectral information of the samples; and step 5, rapid determination: a step of outputting the contents of aflatoxin B1, B2, G1 and G2 and the total amount thereof by using the established model on the basis of the spectral information of to-be-detected brown rice.

The invention discloses a quick detector for quality and safety of tea. The detector is provided with a machine shell of which the upper surface is provided with a color comparison cuvette cover. Thedetector is characterized in that: a transmission type color comparison cuvette and a reflection type color comparison cuvette are arranged side by side under the color comparison cuvette cover of themachine shell; the transmission type color comparison cuvette is a rectangular tank body, one side surface of the tank body is provided with a light source / monochromator, and the other opposite sidesurface is provided with a photoelectric detector; and the reflection type color comparison cuvette is a cuboid of which the inside is provided with three T-shaped channel holes, channel openings of two side channel holes are provided with a light source / monochromator respectively, the lower part channel opening of the middle channel hole is provided with a photoelectric detector, and the lower part channel opening of the middle channel hole is provided with a chromatographic plate. The detector can be used for quickly and quantitatively detecting the contents of heavy metal and pesticide in tea in field and quickly detect the quality safety of tea in field.

The invention discloses an HBV detection method. The method comprises the following steps: separating a nucleic acid extract liquid from serum to obtain a sample to be detected; detecting a reaction result by a fluorescent quantitative PCR instrument, and setting a fluorescence signal as fluorescein corresponding to the 5' end fluorescence group of a Taqman fluorescent probe PgRNA-FP when collected, and collecting the fluorescence signal at 60 DEG C; and carrying out result analysis. Specific reverse transcription is carried out according to HBV PgRNA poly A, and then amplification is carried out according to HBV PgRNA specific primers and the probe, so possible interference caused by HBV DNA is effectively eliminated, and the HBV PgRNA detection specificity is effectively improved.

A device for the controlled filling of containers with powdered substance includes a supply vessel with a narrow exit opening for temporarily holding the powdered substance and for discharging a predetermined quantity of the powdered substance into a container located underneath the supply vessel, a vibrator means is connected to the supply vessel for effecting the discharge of the powdered substance. A sensor for determining the quantity of powdered substance discharged from the supply vessel into the container, and a control unit, which converts the data acquired by the sensor into control commands for the vibrator means is included. The sensor includes a capacitive sensor for the quantitative determination of the quantity of powdered substance falling there through during the filling process and is located between the supply vessel and the container.

The invention discloses a kit for testing lipoprotein a(Lp(a)). The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is a buffer solution, and the reagent R2 is a mixture of lipoprotein a(Lp(a)) antibody sensitized polystyrene latex particles and the buffer solution. The kit has the advantages of simplicity and rapidness in detection, high sensitivity, good accuracy, high anti-interference capacity and low production cost; a method for detecting the lipoprotein a(Lp(a)) is latex enhanced immunoturbidimetry, with the adoption of the method, lipoprotein a(Lp(a)) can be detected more economically, more conveniently and more quickly, and the kit is suitable for automatic biochemical analyzers in most hospitals, and in particular, quick and quantitative detection can be realized during emergency treatment.

The invention discloses a retinol conjugated protein detection kit. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is a buffer solution, the reagent R2 is the mixture of retinol conjugated protein antibody sensitized polystyrene latex particles and the buffer solution. The retinol conjugated protein detection kit has the advantages that the kit is simple and quick to detect, high in sensitivity, good in accuracy, good in anti-interference capacity and low in production cost; a lipoprotein a (retinol conjugated protein) detection method adopted by the kit is a latex enhanced immuno-turbidimetry and enables the lipoprotein a (retinol conjugated protein) detection to be more economical, convenient and quicker, and the retinol conjugated protein detection kit is applicable to automatic biochemical analyzers of most of hospitals and especially can achieve quick quantitative detection for emergency treatment.