265results about How to "Accurate quantitative detection" patented technology

The invention discloses a magnetic particle chemiluminescence micro-fluidic chip. The micro-fluidic chip comprises a top plate (1) and a bottom plate (2), the top plate (1) comprises an air pump (3), a sample adding opening (4), a sample filling area (12), a marking ligand storage pool (5) and a sample mixing area (13). The bottom plate (2) comprises a filter area (6), a magnetic particle coating area (7), a washing area (14), a detecting area (8), a washing liquid storage pool (9), a light-emitting substrate storage pool (10) and a liquid release channel (16). The top plate and the bottom plate both comprise liquid sensing devices, and the liquid sensing devices are used for determining the flowing state of liquid in the micro-fluidic chip and whether bubbles are mixed into the micro-fluidic chip.

The invention discloses a black-smoke car capturing system based on stereoscopic monitoring mode, which comprises a stereoscopic monitoring system of smoke degree, an intelligent video capturing system, an industrial computer and a server platform. The stereoscopic monitoring system of smoke degree and the intelligent video capturing system are connected with service platform through the industrial computer. The smoke stereoscopic monitoring system is used for stereoscopically scanning exhaust smoke of the vehicle, and the scanned image information is sent to the industrial computer; the intelligent video capturing system is used for capturing black smoke video, and the collected video information is sent to the industrial computer. The industrial computer calculates the smoke value and outputs the smoke density distribution diagram after image processing, and sends the capturing command to the intelligent video capturing system when the smoke value exceeds the standard, and carries on the license plate recognition to the video information. The black-smoke car capturing system based on stereoscopic monitoring has the function of capturing the evidence by the video, and has the advantages of accurately giving the smoke value and the opacity value of the automobile exhaust gas, realizing the capturing of the smoky car in the urban key area, reducing the exhaust emission of the urban high pollution motor vehicle and improving the urban air quality.

The invention relates to the technical field of a biosensor, and particularly relates to a fluorescence biosensor for detecting UDG (Uracil-DNA Glycosylase) on the basis of a polymerase-assisted feedback rolling circle amplification and endonuclease amplification fluorescence method. The invention aims to solve problems of both low specificity and low sensitivity of a method for detecting UDG in the prior art. The biosensor for detecting the UDG on the basis of a feedback rolling circle amplification technology achieves a rolling circle amplification effect on matching of phi29 polymerase andendonuclease IV, implements fluorescence resonance energy transfer of a fluorophore and a Quenching group and performs a homogeneous reaction on mixed liquid. A preparation method comprises: constructing a circular template and a composite probe; feeding back a rolling circle amplification signal and carrying out fluorescence detection. Specific hydrolysis of the UDG on a basic group U is utilized, and by utilizing such specific reaction, the UDG can be accurately measured and meanwhile, interference can also be avoided; by utilizing endonuclease IV circle amplification, a signal amplificationeffect is achieved.

The present invention relates to a quantitative detection analysis method of human plasma DNA level. Said method includes the following steps: selecting artificially-synthetic exogenous DNA series whose extraction efficiency is identical to or similar to that of human plasma DNA, but they are non-homologous, selecting housekeeping gene beta actin as determination gene, inserting artificially-synthetic exogenous DNA into carrier pMD18T and placing it in the bacteria to make culture, making blue and white spot screening, colony PCR identification, enzyme-cutting into linearity, placing the above-mentioned material into plasma, adopting double fluorescent quantitative gene amplification, fluorescent groups are respectively FAM and HEX, extracting known exogenous positive plasmid recovery efficiency, using computation formula to make computation so as to obtain the quantity of plasma DNA.

The invention discloses a construction method for a conditional overexpression Yap1 gene mice model at an H11 locus. The construction method comprises the steps: firstly, obtaining an inducible Yap1 overexpression transgenic vector; then, obtaining transgenic F0-generation mice; next, enabling the F0-generation mice to mate with wild-type mice, carrying out passage and line establishment, and thusobtaining F1-generation mice; and carrying out expanding propagation of positive F1-generation mice, then mutually mating, and thus obtaining the conditional overexpression mice Yap1 animal model. The transgenic mice model constructed by the method can be used for studying the function of Yap1 in different tissues and different developmental stages and the action mechanism of Hippo signaling pathways, can also be used for studying the function of the Hippo signaling pathway in animal development, biotumorigenesis, regenerative medicine and other disciplines, and has important guiding significance on biological signal transduction studies.

The invention provides a method for detecting amino acid content in grain by use of terahertz frequency-domain spectrum technology. The method comprises the steps of grinding grain samples to be tested and then tableting the grain samples to be tested, directly testing the grain samples to be tested by use of a terahertz time-domain spectrum system in a transmission measurement mode under a nitrogen atmosphere to obtain the terahertz time-domain spectrum signals of the samples, performing Fourier transform on the time-domain spectrums to obtain the frequency-domain spectrums of the samples, performing normalization spectrum preprocessing on all the obtained frequency-domain spectrums, and finally, dividing the preprocessed frequency-domain spectrums of the grain tableted samples to be tested into calibration set frequency-domain spectrums and verification set frequency-domain spectrums, and establishing a quantitative analysis model by use of the partial least squares regression method so as to obtain the quantitative detection values of the various grain samples to be tested. The method for detecting the amino acid content in the grain by use of the terahertz frequency-domain spectrum technology is simple in sample preparation, simple to operate, and capable of truly and effectively realizing fast and accurate quantitative detection on the amino acids in the grain.

The invention provides a microdroplet-type digital PCR detection kit for a latent hepatitis B virus. The kit comprises digital PCR reaction liquid and a primer probe composition, and the primer probecomposition is selected from at least one of an S gene area primer probe composition and a C gene area primer probe composition. According to the kit, primer probes are respectively designed for S andC conserved sequences of the hepatitis B virus, a duplex double-gene digital PCR technology is adopted to synchronously detect HBV DNA in the same tube, and the operation is convenient and quick; thesensitivity and specificity of a detection method for the latent hepatitis B virus are improved, the probability that the false negative result is caused due to mutation is reduced as much as possible, and the detection efficiency can be further improved.

The invention discloses a composite insulator ageing photo-thermal radiation detection system based on an unmanned aerial vehicle. The system comprises an airborne terminal 9 and a ground terminal 14,the airborne terminal comprises an unmanned aerial vehicle body 1, a power module 2, a flight control module 3, a satellite positioning module 4, a cloud deck 5, a photo-thermal radiation detection module 6, an airborne wireless transmission module 7 and an airborne information processing center 8, and the ground terminal comprises a ground wireless transmission module 10, a ground unmanned aerial vehicle control module 11, an image processing and displaying module 12 and a ground information positioning center 13. According to the system, the unmanned aerial vehicle carries the photo-thermalradiation detection module to detect temperature distribution time change or space distribution conditions of a composite insulator after laser radiation, the relationships among thermal conduction characteristics and aging degree of the composite insulator are analyzed, and photo-thermal radiation online remote sensing evaluation of an ageing state of the overhead composite insulator is achieved. The system has the advantages that the method and operation are simple, non-contact, lossless, high-sensitivity, efficient, online remote sensing evaluation of the ageing degree is achieved and thelike.

The invention discloses a center hole out-of-roundness detection method of shaft parts. The method includes the following steps that a spare tip of a machine tool is transformed into a three-point tip for detection; the three-point tip is arranged on a machine tool headstock for grinding a workpiece to form a headstock three-point tip; the workpiece to be subjected to center hole out-of-roundness detection is hoisted to the machine tool to be clamped and the headstock three-point type tip and the workpiece are marked with marks through a marking pen; a machine tool button is jogged, a heart-shaped clamping and poking rod of the machine tool drives the workpiece to jog and rotate; the runout value A1 of the portion closest to the shaft neck of the headstock three-point type tip is measured; the steps mentioned above are repeated, then the machine tool button is jogged again to stop the workpiece from rotating, the workpiece is poked manually to rotate clockwise by about 90 degrees, 180 degrees and 270 degrees, and the runout value A2, the runout value A3 and the runout value A4 are recorded; and the Amax-Amin difference value serves as the center hole out-of-roundness judgment value. By the adoption of the center hole out-of-roundness detection method, the precise detection purpose of center hole out-of-roundness of the shaft parts can be well achieved, and the clear and operable control requirements can be met for control over center hole out-of-roundness.

The invention discloses a method for synchronously extracting and purifying various chlorinated polycyclic aromatic hydrocarbons in soil, which belongs to the field of detection of the chlorinated polycyclic aromatic hydrocarbons. The method comprises the following steps: firstly removing moisture in a soil sample by virtue of a freezing dryer, grinding the soil sample, and filtering the ground soil sample by virtue of a 200-mesh sieve for later use; and extracting a target matter in the soil sample by utilizing an acceleration solvent extractor, purifying the target matter in a gel permeation chromatography, finally concentrating the target matter and sizing the target matter. According to the method, a preprocessing method of the chlorinated polycyclic aromatic hydrocarbons in the soil is established, less solvent is used in the entire process, the automation degree is high, less time is used, the safety is high, fewer steps are used, and various chlorinated polycyclic aromatic hydrocarbons in the soil can be accurately, qualitatively and quantitatively determined. The chlorinated polycyclic aromatic hydrocarbons in the soil are quantitatively detected by utilizing the gas phase chromatography-mass spectrometry, the detection limit is low (when S/N is equal to 3, the detection limit is 2.6 to 25pg/g), the precision is high (RSD=6.30 to 12.0 percent), the blank mark recovery rate is 64.1 to 109.2 percent, the synchronous analysis and detection of various trace chlorinated polycyclic aromatic hydrocarbons in the complicated environment medium such as the soil can be realized, sensitivity and accuracy can be realized, and the weaknesses of the prior art can be overcome.

The invention belongs to the field of in-vitro diagnosis, and particularly relates to a test strip card which can filter samples and is provided with a storage medium (14) for storing a tested object standard curve, coefficient parameters or other detection information. The test strip card comprises a card box (3) and a test strip (2) in the card box (3), wherein a storage medium (14) is installed on the card box (3). When detecting a sample, an instrument with signal detection function acquires characteristic signals of a test strip (2) detection belt (7) and calculates the sample single component or multicomponent concentration in combination with the tested object standard curve or coefficient parameters simultaneously read from the storage medium (14) by the instrument. The test strip card for detecting samples has is simple and quick, and has the characteristics of high sensitivity, objective result, high use flexibility and the like.

The invention relates to a Q245R material nondestructive stress test method. By taking the measured coercivity value of the device under test into different formulas in different states, the stress value of the device under test can be obtained. The method can realize accurate and quantitative testing of stress states of Q245R materials. In the invention, according to the characteristics of the magnetization of the Q245R materials and the magnetic memory parameters, the test efficiency is high; tests can be carried out repeatedly to facilitate the re-inspection. The method of the invention can carry out the non-destructive field nondestructive testing on the equipment and pipelines produced with the Q245R materials; the detection process is simple and effective; and requirements for surface conditions of the equipment are not high, thereby saving the testing cost. The method of the invention makes clear the load states of the pipelines, equipment, etc. to prevent accidents related to stress synergy so as to reduce enterprise losses and bring obvious social benefits.

The invention discloses a 24-site microarray screen printing electrochemical sensing device, which comprises a sliding layer arranged in sequence from top to bottom, an insulating layer used to limit the reaction area, a base layer and a support layer used to fix the whole device , the base layer and the insulating layer are fixed on the support layer, the sliding layer and the support layer are slidingly connected, and the conductive printed circuit is collected at the end of the base layer and combined with the joint to connect the electrochemical workstation; and discloses application of this device. The advantage of the present invention is that it combines the traditional immune technology with the sensor technology, and provides a new fast, convenient and sensitive detection method for the trace detection of small molecular substances enrofloxacin and melamine, and can simultaneously realize multi-channel, Detection of multiple samples. It overcomes the technical problems of the original detection method, such as only targeting a single detection object, long detection time, expensive instruments, complicated operation, low sensitivity, poor specificity, and small detection range.