The invention relates to a method for integrating an exogenous gene into a sheep listeria genome. The method comprises the following steps of: electro-transforming a first target recombinant plasmid into a first recombined bacterium, and then performing single / double homologous recombination hybridizing cultivation on the bacterium which is obtained after transforming, thereby obtaining a first target bacterium, wherein the first target bacterium is a recombined sheep listeria, the exogenous gene is integrated with an orfBAldh gene in the genome, and a code protein of the exogenous gene is stably expressed and secreted; or electro-transforming a second target recombinant plasmid into a second recombined bacterium, and then performing single / double homologous recombination hybridizing cultivation on the bacterium which is obtained after transforming, thereby obtaining a second target bacterium, wherein the second target bacterium is a recombined sheep listeria, actA gene and plcB gene are not in the genome, the exogenous gene is integrated with the orfBAldh gene in the genome, and the code protein of the exogenous gene is stably expressed and secreted.