The invention discloses a method for promoting a target
rhizobium to migrate and colonize in an alfalfa
plant. An endogenous
fluorescence labeling
rhizobium R. GN5f liquid with cyan
fluorescent protein (CFP) characteristics is obtained through a tri-parent conjugation method, natural
plant source
bacteriostatic agent matrine is added, and the endogenous
fluorescence labeling
rhizobium R. GN5f liquid and the
matrine are mixed and inoculated to the root of gannong No.5 alfalfa in the reproductive growth stage. The method for promoting the target rhizobium to migrate and colonize in the alfalfa
plant is advantaged in that (1), the target rhizobium R. GN5f is obtained through the tri-parent conjugation method, is good in
genetic stability and convenient to use, and can be obtained easily; (2) the
matrine restrains air source and soil source microorganisms, and does not restrain rhizobium, and promotes the migration and
colonization of the rhizobium in the alfalfa, and is low in price, and is innocuous and unpoisonous to the environment; (3) the
fluorescence labeling rhizobium liquid containing the matrine can be obtained easily, the
inoculation operation is simple, short in consuming time, and
inoculation can be carried out at any moment in the growth period; and (4) by adding the matrine, the migration and
colonization of the target rhizobium are promoted, and the foundation is laid to obtain seeds with a lot of target
rhizobia.