44results about How to "Good antiviral activity" patented technology

The invention relates to gene segments for coding alpha, beta or gamma interferons of pigs and an application thereof. According to the preference of codons of a Pichia stipitis expression system, high-expression codons are selected to renewedly design and synthesize new mature peptide nucleotide sequences of the alpha, beta and gamma interferon of the pigs, and recombinant yeast expression plasmids of pPICZalphaC-IFN-alpha, pPICZalphaC-IFN-beta and pPICZaplhaC-IFN-gamma are successfully constructed, which not only correctly expresses the alpha, beta and gamma interferons of the pigs in Pichia stipitis, but also enables the alpha, beta and gamma interferons of the pigs to be easier to express in the Pichia stipitis with higher expression level, and lays a foundation for the large-scale production and fermentation of the gene engineering.

The invention discloses sesquiterpenoids with anti-influenza virus activity and applications. The sesquiterpenoids are shown in formula (1-14). The present invention obtains 14 sesquiterpenoids through separation and screening, and Through the antiviral activity study, it is found that these sesquiterpenoids have good anti-influenza virus activity, and the selectivity index of some compounds against influenza virus is greater than 500, showing a good research prospect.

The invention relates to a preferred rice black-streaked dwarf virus RNAi multivalent target gene sequence which sequentially comprises 206 bp segment in a rice black-streaked dwarf virus S8 code region, 205 pb in an S10 code region, 203 pb in an S4 code region, wherein a BamH1 restriction enzyme cutting site and three CCG protective bases are added at the 5'end of the S8; an XhoI restriction enzyme cutting site and three ACA protective bases are added at the 3'end of S4. According to the rice black-streaked dwarf virus RNAi multivalent target gene sequence and application, based on a special design and optimization and specific to common 614 bp of RANi target sequence of three genes, namely RBSDV S8, S10, and S4, the specific rice black-streaked dwarf virus RNAi multivalent target gene sequence is acquired, cloned and structured into an RNAi carrier pBDL03 to obtain an RNA silent carrier pBDL03-S8+S10+S4; then RNA silent carrier pBDL03-S8+S10+S4 is transferred to a rice variety Taigeng 394 through an agrobacterium transformation method. T1 generation positive plants have favorable resistance on RBSDV.