300 results about "Enterovirus" patented technology

Lactobacillus rhamnosus and the use for it and its metabolin are disclosed. The strain is sieved and obtained from excrement of long-lived old. It's safe, has excellent antibacterial, acid-resisting and antioxidant performances. It can be used in dairy process and functional food production.

Antiviral protease inhibitors, including peptidyl aldehydes, peptidyl α-ketoamides, peptidyl bisulfite salts, and peptidyl heterocycles, are disclosed, along with related antiviral compounds, and methods of using the same to treat or prevent viral infection and disease. The compounds possess broad-spectrum activity against viruses that belong to the picornavirus-like supercluster, which include important human and animal pathogens including noroviruses, enteroviruses, poliovirus, foot-and-mouth disease virus, hepatitis A virus, human rhinovirus (cause of common cold), human coronavirus (another cause of common cold), transmissible gastroenteritis virus, murine hepatitis virus, feline infectious peritonitis virus, and severe acute respiratory syndrome coronavirus.

The invention relates to the field of biomedicine, in particular to an enzyme-linked immunization diagnostic reagent kit for detecting an enterovirus (EV) 71-type antibody (immune globulin M (IgM)), and a preparation method and application of the diagnostic reagent kit. The probability of hand-foot-and-mouth disease and severe infection (viral encephalitis, viral cerebrospinal meningitis and pulmonary edema) caused by EV71 type is relatively higher, and case fatality rate is relatively higher and can be 10 to 25 percent. The enzyme-linked immunization diagnostic reagent kit of the EV71-IgM antibody can be used for diagnosing the infection of the EV71 type. According to related documents about the detection of the EV71-IgM, EV71 virus cultures serving as indirect enzyme-linked immuno sorbent assay (ELISA) of envelope antigens has defects in such aspects as specificity, sensitivity and stability, and due to high cultivation cost and low efficiency, a large amount of virus cannot be supplied to the market. In order to overcome the defects, the invention provides the reagent kit which is used for detecting the EV71-IgM in human blood serum, required by clinical examination, simple and convenient to operate and applicable to all medical disease control departments, and the preparation method and the application of the reagent kit. The invention has the technical scheme that: firstly, the human blood serum is added into a micro-pore plate, wherein the IgM antibody is obtained by an anti-mu chain which is pre-enveloped on the micro-pore plate, and other uncombined components are washed and removed; secondly, an enzyme labeling object is added, the EV71-IgM in the obtained IgM can be combined with the specificity of an EV71 recombinant antigen which is labeled by horse radish peroxidase (HRP), and after washing, the HRP can react with substrates which are added subsequently; and finally, the aim of detecting the EV71-IgM antibody is fulfilled.

The invention discloses a quick test paper for detecting enterovirus and a method for preparing the same. The test paper detects enterovirus EV71 and Coxsackievirus A16 viruses in a sample by the immunochromatography adopting marking by colorful particles. The quick test strip is formed by sequentially overlapping and bonding a sample adsorption liquid layer, a colorful particle storing pad, a cellulose nitrate membrane and a water adsorption board on a bottom board, wherein the colorful particle storing pad is coated with a monoclonal antibody with a colorful particle mark; and the cellulose nitrate membrane is provided with a detection line sprayed by the monoclonal antibody of the EV71 and / or Coxsackievirus A16 and a control line sprayed by the polyclonal antibody of anti-mouse IgG. The test paper can quickly detects the EV71 and Coxsackievirus A16 viruses in the detected sample at the same time or respectively, finds the epidemic situation caused by the virus infection as early as possible and has the advantages of being easily operated, quickly getting results, avoiding special operators and the like.

Antiviral protease inhibitors, including macrocylic transition state inhibitors and peptidomimetics are disclosed, along with related antiviral compounds, and methods of using the same to treat or prevent viral infection and disease. The compounds possess broad-spectrum activity against viruses that belong to the picornavirus-like supercluster, which include important human and animal pathogens including noroviruses, sapoviruses, enteroviruses, poliovirus, foot-and-mouth disease virus, hepatitis A virus, human rhinovirus (cause of common cold), human coronavirus (another cause of common cold), transmissible gastroenteritis virus, murine hepatitis virus, feline infectious peritonitis virus, and severe acute respiratory syndrome coronavirus.

Antiviral protease inhibitors, including peptidyl aldehydes, peptidyl α-ketoamides, peptidyl bisulfate salts, and peptidyl heterocycles, are disclosed, along with related antiviral compounds, and methods of using the same to treat or prevent viral infection and disease. The compounds possess broad-spectrum activity against viruses that belong to the picornavirus-like supercluster, which include important human and animal pathogens including noroviruses, enteroviruses, poliovirus, foot-and-mouth disease virus, hepatitis A virus, human rhinovirus (cause of common cold), human coronavirus (another cause of common cold), transmissible gastroenteritis virus, murine hepatitis virus, feline infectious peritonitis virus, and severe acute respiratory syndrome coronavirus.

A method is provided for reverse transcription-polymerase chain reaction (RT-PCR) comprising a) amplifying a reverse transcribed cDNA in a mixture comprising Norovirus Genogroup I and Norovirus Genogroup II primers and probes, wherein said Norovirus primers and probes distinguish between Genogroup I and Genogroup II viruses; b) quantifying virus; and c) normalizing data based on a universal internal RNA control. Optionally, the method may also include primers and probes for Enteroviruses. A reaction mixture comprising Norovirus Genogroup I and Norovirus Genogroup II primers and probes, wherein said Norovirus primers and probes distinguish between Genogroup I and Genogroup II viruses and universal internal RNA control primers and probes are also included.

The invention discloses a high-flux non-diagnostic detection method for 13 respiratory viruses based on a novel suspension chip technology. Aiming at the specificity gene sequences of 13 pieces or types of respiratory viruses, such as influenza viruses A and B, auxiliary influenza viruses 1 and 3, coronavirus SARS-CoV and CoV-NL63, respiratory syncytial viruses A and B, adenoviruses B and E, a rhinovirus, a human metapneumovirus and an enterovirus, 13 pairs of primers and corresponding specificity probes are designed; by multiple polymerase chain reaction (PCR) amplification and TSPE reaction, X-TAG nucleic acid fragment and biotin are respectively marked with corresponding gene fragments; the marked fragments are combined with corresponding encoded micro balls; and a suspension chip scanner is used for performing detection. The high-flux non-diagnostic detection method can accurately detect 13 respiratory viruses and subtypes of the 13 respiratory viruses; the probes do not generate cross reaction; the specificity is high; and the sensitivity is high. A high-flux, quick and accurate technical platform is capable of simultaneously detecting various respiratory viruses; technical equipment is supplied to the detection of pathogens of sudden respiratory tract infectious diseases; and information can be timely supplied to the handling of infectious disease epidemic situation burst.

The invention relates to an enterovirus type 71 (abbreviated as EV71) and use thereof, in particular to a monoclonal virus strain which is separated by a plaque assay method. The progeny viruses of the monoclonal virus strain show genetic stability. The monoclonal virus strain can be used in the production of a vaccine (particularly the production of a vaccine for infant). The virus strain or the vaccine produced by using the same can be used in the prevention of diseases (such as hand-foot-and-mouth diseases, particularly hand-foot-and-mouth diseases in children) caused by the EV71 and has the characteristics of stable titer, high immunogenicity and small immunizing dose.

Antiviral protease inhibitors, including macrocylic transition state inhibitors and peptidomimetics are disclosed, along with related antiviral compounds, and methods of using the same to treat or prevent viral infection and disease. The compounds possess broad-spectrum activity against viruses that belong to the picornavirus-like supercluster, which include important human and animal pathogens including noroviruses, sapoviruses, enteroviruses, poliovirus, foot-and-mouth disease virus, hepatitis A virus, human rhinovirus (cause of common cold), human coronavirus (another cause of common cold), transmissible gastroenteritis virus, murine hepatitis virus, feline infectious peritonitis virus, and severe acute respiratory syndrome coronavirus.

The invention relates to probes and assays which are used for the simultaneous detection, in a single assay sample, of a plurality of nucleic acid sequences of viruses that cause respiratory infections in humans, selected from among influenza virus type A, influenza virus type B, influenza virus type C, human respiratory syncytial virus type A, human respiratory syncytial virus type B, human adenovirus, human parainfluenza virus type 1, human parainfluenza virus type 2, human parainfluenza virus type 3, human parainfluenza virus types 4A and 4B, enterovirus, rhinovirus, human coronavirus type 229E, human coronavirus type OC43, coronavirus that causes severe acute respiratory syndrome (SARS), human metapneumovirus and combinations thereof.

The present disclosure relates to methods of producing Enterovirus A, e.g., for vaccine production, that include culturing cells in a fixed bed bioreactor. Further provided herein is an Enterovirus A produced by the methods of production disclosed herein, as well as compositions, immunogenic compositions, and vaccines related thereto.

The invention discloses a traditional Chinese medicine for treating bacterial diseases in poultry, which is prepared from the following raw materials in parts by weight: 10-15 parts of wild chrysanthemum flower, 5-10 parts of Chinese goldthread, 8-10 parts of Scutellaria baicalensis, 8-10 parts of golden cypress, 10-15 parts of Chinese thorowax, 10-12 parts of Cape jasmine, 20-25 parts of Fagopyrum cymosum root, 25-30 parts of common andrographis herb, 8-10 parts of burdock, 25-30 parts of Chinese pulsatilla root and 30-35 parts of folium artemisiae argyi. The traditional Chinese medicine for treating the bacterial diseases in the poultry, disclosed by the invention, has the effects of preventing and treating the bacterial diseases caused by colibacillosis, salmonellosis, necrotizing enterocolitis, enterovirus syndromes, cholera fowls and the like in the poultry.

The invention discloses a double-fluorescent PCR detection primer, a probe, reaction liquid and a kit capable of rapidly detecting 16 pathogens of the respiratory tract. The kit comprises pre-subpackaged PCR reaction liquid, RT-PCR enzyme, a positive quality control product and a negative quality control product, wherein the pre-subpackaged PCR reaction liquid is provided with a primer and a TaqMan probe for detecting at least two of the following pathogens: respiratory syncytial virus, enterovirus, coronavirus NL63, coronavirus HKU1, coronavirus 229E, coronavirus OC43, parainfluenza virus type I, parainfluenza virus type II, rhinovirus, parainfluenza virus type III, human bocavirus, human metapneumovirus, mycoplasma pneumoniae, chlamydia pneumoniae, adenovirus and legionella pneumophila. The kit is convenient to operate, and can be used for at most screening 16 syndrome pathogens of the respiratory tract within 2 hours, so that the detection time and cost are greatly saved. The kit has the greatest advantages of simplicity in operation and strong practicability, and can be easily popularized in laboratories of the ports.

The present invention provides a monoclonal antibody capable of neutralizing EV71 infection.

The invention relates to the pharmacy field, concretely relates to a use of a series of pentacyclic triterpene compounds as enterovirus EV71 inhibitors, and especially relates to an application in the preparation of medicines for preventing and treating EV71 infection induced hand-foot-and-mouth diseases and complications thereof, such as synanche, myocarditis, pulmonary edema, encephalitis, herpes, septicemia, hypertension, hyperglycemia, cognitive function disorder, poliomyelitis-like paralysis and many nerve system associated diseases. The invention also discloses medicinal compositions of the series of the pentacyclic triterpene compounds as enterovirus EV71 inhibitors.

The invention provides polynucleotides and methods for detecting and quantifying RNA viruses, such as enteroviruses and noroviruses. In one aspect, the invention provides amplification primers and labeled molecular beacons for amplification of viral nucleic acid sequences. In another aspect, the invention provides a synthetic RNA internal control. In another aspect, the invention provides a kit for detecting the presence of enterovirus and / or norovirus in a sample.

The invention relates to the fields of medicinal chemistry and pharmacotherapeutics, in particular to compounds of a general formula (I), whichserve as an enterovirus protease inhibitor. The compounds have remarkable inhibition activity on coronavirus [SRAS (severe acute respiratory syndromes)] main proteinase and can be used for treating related diseases. The invention also relates to a preparation method of the compounds, a medicinal composition of the compounds, and medicinal salt, enantiomeric form and diastereoisomer as well as racemic compounds. (The formula I is as shown in the description).

The invention belongs to the technical field of animal tissue isolation and culturing, and particularly relates to a method of porcine intestinal crypt isolation and 3D type organ culturing. In the course of porcine intestinal crypt isolation, penicillin and streptomycin are added, so that in the later-stage culturing course, pollution caused by intestinal microorganisms is avoided; rotating speedand time for treating an intestinal segment of a horizontal rotary instrument and frequency and time of a vortex of a vortex instrument are optimized, so that the obtained crypt is complete in shape,and higher in survival rate and bud rate. According to the method disclosed by the invention, a formula of a culture solution for culturing the crypt and the 3D type organs is optimized, so that thecrypt can be well polarized to form the 3D type organs, and the growth and the polarization are stable; the isolating condition and the optimal culturing method of the porcine intestinal crypt are determined, and favorable foundation for subsequently utilizing the porcine intestinal 3D type organs to study enterovirus and bacterial infection is established.

The invention provides a multiple PCR detection kit for pathogens of respiratory tract, an application and an application method thereof. The PCR detection kit comprises a mixture of various primers and probes, a PCR reaction liquid, a PCR enzyme liquid, an inner quality control product and a nucleic acid extraction reagent. The detection kit is respiratory syncytial virus used for detecting one or more pathogens of influenza a virus, influenza B virus, respiratory syncytial virus, parainfluenza I, parainfluenza II, parainfluenza III, adenovirus, human metapneumovirus and enterovirus or rhinovirus. The application of the kit comprises the following steps: firstly, carrying out sampling; then carrying out PCR amplification and detection; and finally, obtaining a detection result. By combingand coordinating the nucleic acid extraction reagent, the primer pairs, the probes, a PCR program, the PCR reaction liquid and the PCR enzyme liquid, the technical effects of shortening the detectiontime and being good in detection sensitivity and good in specificity are achieved finally.

The invention relates to a Chinese medicine composition for treating enterovirus syndrome in chicken, which mainly comprises ten kinds of Chinese herbal medicines, such as sweet wormwood, antipyretic dichroa, Chinese pulsatilla root, lightyellow sophora root, golden cypress and the like. The Chinese medicine composition has the actions of killing inspects, sterilizing and benefiting qi and enriching blood and has an obvious effect on the enterovirus syndrome caused by coccidium and bacterial infections. The Chinese medicine composition has the advantages of easiness of acquirement of medicinal materials and convenient preparation process and is suitable for industrial production.

The invention aims at providing chimeric protein of enterovirus EV71 neutralization epitope and a human norovirus P structure domain and an establishing method of a chimeric vector. By means of immunoblotting, it is verified that the chimeric protein of the enterovirus EV71 neutralization epitope and the norovirus P structure domain can be used for research and development of detection kits of EV71 and norovirus and novel bivalent subunit vaccines.

The invention discloses a compound dimetridazole premix for preventing and treating the poultry enterovirus syndrome. The premix is prepared by uniformly mixing the following components in percentage by weight: 10 to 30 percent of dimetridazole, 4 to 10 percent of neomycin sulfate, 2 to 8 percent of decoquinate econazole nitrate, 10 to 30 percent of lysozyme, 0.5 to 5 percent of etamsylate, 3 to 10 percent of lactic acid TMP and the balance of pharmaceutically applicable auxiliary material. According to the invention, the novel high-efficiency anticoccidial drug decoquinate econazole nitrate, the dimetridazole and the neomycin sulfate which have a special curative effect on intestinal infection, the lysozyme with effects of resisting to bacteria, resisting to viruses, diminishing inflammation, relieving pain and repairing intestinal mucosa, the high-efficiency hemostatics etamsylate and the drug synergist are compounded into a compound preparation; and the compound dimetridazole premix has a high-efficiency treatment effect on the poultry enterovirus syndrome. The compound dimetridazole premix has a reasonable and scientific formula, is simple to prepare, has wide application and can be used as a priority drug for preventing and treating the poultry enterovirus syndrome.

The invention belongs to the technical field of biological pharmacy, and particularly relates to a water extract of houttuynia cordata thunb and a preparation method and an application of the water extract. Total polysaccharide obtained from the water extract of the houttuynia cordata thunb has activity to inactivate or inhibit replication of hand-foot-mouth disease viruses, namely an enterovirus-71 (EV-71) and coxsackie A-16 (CVA16); polysaccharide with the antiviral activity is further found to be polysaccharide with molecular weight greater than 100kDa; therefore, the polysaccharide can be applied in preparation of a medicine for preventing the hand-foot-and-mouth disease viruses. As an edible aquatic herbaceous plant, the houttuynia cordata thunb has the characteristics of extensive source, small toxic and side effects, low price, simple extraction process and the like, and has important development and application prospects.

The invention discloses a composition used for detecting enterovirus causing hand foot and mouth disease, which comprises an upstream primer P1:5'-CCCTGAATGCGGCTAATCC-3' and a downstream primer P2:5'-GTCGGTTCCGCTGCAGAGT-3'. Genetic fragments of 5'UTR regions of EV71 and Cox A16 type enteroviruses in a sample can be specifically detected by the composition matched with a proper probe and other reagents through a real time Polymerase Chain Reaction (PCR) method. The invention also discloses a kit containing the composition, and a method for detecting the EV71 and / or Cox A16 type enterovirus by utilizing the composition or the kit. When the composition or the kit and / or the method is utilized for detecting the sample, whether the EV71 and / or Cox A16 type enterovirus exist in the sample can be sensitively and rapidly detected in a reaction; therefore, the efficiency and the sensitivity of the detection are improved, the detection process is simplified, and the manpower, the material resources and the detection cost are saved.

The invention discloses an isothermal amplification method for enterovirus EV nucleic acid. The method comprises the following specific steps of: amplifying in a reaction system, wherein the reaction system contains a specific primer pair which amplifies enterovirus EV, and the primers can amplify amplifying products corresponding to characteristic sequences of enterovirus EV from a detection sample with extremely low EV copy number. The method provided by the invention can be used for amplifying the sample to be tested containing EV RNA quickly with high specificity, high sensitivity and low pollution, and the characteristics of high detection efficiency and high accuracy as well as wide application prospect.

The invention discloses a method for detecting an enterovirus neutralizing antibody and a special recombinant virus for the method and provides a DNA (deoxyribonucleic acid) molecule. The DNA molecule is a recombinant DNA obtained by substituting coding genes of all structural proteins in cDNA (complementary DNA) corresponding to genome RNA (ribonucleic acid) of an enterovirus for report genes. An EV71 FY pseudovirus system is used for the method for detecting the neutralizing antibody, and since pseudoviruses subjected to monocyclic infection are adopted, the safety problem during using of live viruses is avoided. After multiple tests, results show that the pseudovirus system is the method for detecting the neutralizing antibody and is safe, sensitive, rapid, specific, simple, convenient and low in cost. Based on the advantages, the pseudovirus system is extremely suitable for rapid and massive neutralizing antibody detection tests, and has important application values for virus vaccine development and detection of hand-foot-and-mouth disease specificity neutralizing antibody level of individual patients and patient populations.

The invention discloses multiple primers and a kit for high-throughput detecting of a whole genome of enterovirus, and a high-throughput sequencing analysis method. Compared with the prior art, specific primers do not need to be used for detecting a certain generic type one by one in a targeted mode any more, but multiple PCR is utilized to conduct genomic amplification on various enterovirus subtypes through an experiment, the virus subtypes are accurately distinguished and identified, and the accuracy rate is higher, and repeatability is better; high-depth sequencing is adopted, the enough detection depth is ensured, and thus false positive is lower; and through the detection method, the gene types of the enterovirus are rapidly identified, genome variable site, diversity and recombination analysis of viruses can be further provided, and the important scientific basis of prevention and control over the enterovirus is provided.

The invention discloses primers for screening and parting enterovirus viruses and a detection method. The sequences of the primers are the CODEHOP outer primer sequence, the CODEHOP inner primer sequence and the primer sequences of 20 viruses. The detection method includes the steps of extraction of RNA; one-step RT-PCR; nest type PCR; 1.5% agarose gel electrophoresis to judge the positivity and parting result of enteroviruses. A pair of enterovirus consistent-degenerate primers is designed, a pair of short-fragment consistent-degenerate primers and parting primers of 20 different enteroviruses are designed inside amplified fragments of the primers, and in combination with the nest type RT-PCR technology, a degenerate primer nest type RT-PCR method capable of being used for screening and parting different genotype viruses of the enteroviruses is established. The advantages of being high in sensitivity and specificity and suitable for monitoring and variety identifying of enteroviruseses of low-virus-content samples are achieved.