1673results about How to "Simple extraction process" patented technology

The effective plant component extracting process includes mechanically crushing the mixture of solid plant material and proper amount of chemical assistant, and the subsequent extraction of the effective component with water or alcohol. The mechanical crushing can produce micro shearing strain in the interface, break cell wall, expose fresh cut surface and produce mutual adsorption and other action between the grain clusters and molecular groups of the chemical assistant and the effective component, so as to alter the microscopic performance of the effective plant component, raise the leached amount by 80-400 %, short the extracting period greatly, raise the extracting efficiency obviously raise the extraction specificity and selectivity, minimize the organic solvent in use, lower the extraction cost and raise the bioavailability of the extracted matter.

The invention relates to the field of deodorization, and especially relates to a plant extract and microbe composite deodorant. According to the invention, a plant extract and a microbial fermentation broth are mixed according to a ratio of 1:3; the mixture is placed in plastic buckets; and sealed anaerobic fermentation is carried out for 5-9 days, such that the composite deodorant is obtained. The microbial fermentation broth is obtained through the following steps: saccharomycetes, lactobacillus, bacillus subtilis, acetobacter pasteurianus and photosynthetic bacteria are inoculated according to the ratio of 1-4:2-6:5-25:1-4:1-2, and mixed fermentation is carried out; and after 60-84h of aerobic fermentation, the microbial fermentation broth is obtained. The invention aims at providing the plant extract and microbe composite deodorant. Deodorizing is realized with the combination of the plant extract and microbes. With the deodorant, fast and continuous deodorization can be realized; production cost is reduced; and a deodorization effect is good. With the deodorant, deodorization can be achieved fundamentally.

The invention provides a wave energy and ocean current energy integrated generator comprising an undersea guide caisson, a water turbine, a vibrating floater, a round void compartment, a fixing rack, a hydraulic integrated generating module, and three hydraulic cylinders. The water turbine is disposed in the undersea guide caisson. The vibrating floater disposed outside a transmission shaft of the water turbine floats on the water surface. The round void compartment is mounted on the transmission shaft and arranged above the vibrating floater. The fixing rack is arranged on the outer surface of the round void compartment. The hydraulic integrated generating module is disposed in the fixing rack. The hydraulic cylinders are disposed between the vibrating floater and the fixing rack and convert ocean current energy and wave energy from sea water into electric energy through the water turbine and the vibrating floater, respectively. The wave energy and ocean current energy integrated generator is capable of extracting wave energy and ocean current energy at the same time, the generating process is continual and controllable, the generator is high in operational reliability, output electricity quality is high, and economic efficiency is good; in addition, the invention further provides a power generation method using the wave energy and ocean current energy integrated generator.

The invention relates to extraction of natural gromwell dye and application thereof. The extraction comprises the following steps of: crushing gromwell, ultrasonically extracting the crushed gromwell at a certain temperature by using ethanol solution as a solvent to obtain pasty natural gromwell dye, and adding an emulsifying agent into the pasty natural gromwell dye to perform emulsification so as to obtain aqueous solution of the natural gromwell dye. The natural gromwell dye used for dyeing protein, cotton, polyester fibers, yarns or fabrics has good dyeing effect. The natural dye is extracted from the gromwell, and the solvent is reclaimed for reuse, so the environment is not polluted; and the dye extraction process is simple, and the natural gromwell dye has stable quality and broad market prospect.

The invention relates to an extraction method of spirulina phycocyanin. The method comprises the following steps of: processing spirulina powder suspension by adopting a freeze thaw method and a machine crushing process to obtain spirulina cell disruption liquid; performing fractional salting out to precipitate the spirulina cell disruption liquid with 30 percent ammonium sulfate liquid and 50 percent ammonium sulfate liquid to obtain phycocyanin coarse extract liquid 1; adding PEG 20000 (polyethylene glycol 20000) and NaCl to further precipitate out phycocyanin coarse extract with higher purity; dissolving the phycocyanin coarse extract with phosphate buffer to prepare phycocyanin coarse extract liquid 2; and extracting the phycocyanin coarse extract liquid with PEG 20000 / Na2SO4 aqueous two-water-phase system, and desalting extracted lower phase by dialysis to obtain phycocyanin fine extract liquid, and lyophilizing to prepare a phycocyanin finished product. The method is simple in extraction process and low in cost, and is suitable for intermittent and scale production processing of high-purity and high-yield spirulina phycocyanin finished product. The phycocyanin coarse extract can be stored for a long time, and the phycocyanin finished product which is prepared by further purification has excellent antioxidant free radical scavenging effect and fluorescent strength.

The invention discloses a cosmetic containing oceanic shellfish active peptide and a preparation method and application thereof. The cosmetic contains 0.1 to 10 percent of oceanic shellfish active peptide, or 0.1 to 10 percent of oceanic shellfish active peptide and 1 to 10 percent of hyaluronic acid, or 0.1 to 10 percent of oceanic shellfish active peptide, 1 to 10 percent of hyaluronic acid and1 to 25 percent of vitamin C (VC), and the balance is cosmetic matrix components. The peptide is formed by boiling, ultrasonic, enzymolysis and ultra-filtration of oceanic shellfish meat; and besidesthe active peptide, the oceanic shellfish meat also contains free amino acid, glycosaminoglycan, polyunsaturated fatty acid, vitamins, trace elements and the like. The problems of application and thelike of the oceanic shellfish active peptide in the cosmetic are solved; and the prepared cream, emulsion, aqueous agent, gel, aerosol or mask has more complete and remarkable skin-care or hair-care effect. After the hyaluronic acid and the VC are added, the effects of preserving moisture, increasing the elasticity and the smoothness of the skin, removing flaws and improving the skin color are remarkably enhanced.

An application of naringenin and its salt in preparing the medicines for treating cough and resolving phlegm is disclosed. Said naringenin is prepared from naringin through hydrolysis or from the natural medicinal materials through extracting, or by chemical synthesis. Its advantages are high curative effect and no toxic by-effect.

The invention relates to a making method of instant squid food. The making method comprises the steps of pretreatment, soaking, tenderization, stewing, flavoring, baking and package. The making method has the advantages that before squids are made, the squid meat is soaked in tea polyphenol solution, residual formaldehyde can be removed, and meanwhile, the squid meat can be prevented from discoloring through oxidation; before the squid meat stewing, the squid meat is subjected to tenderization treatment so that the original muscular tissue structure of the squid meat is destroyed, the made instant squid food has good mouth feeling, excellent taste and good palatability, and the selection of mass consumers is met; and the storage of the used tea polyphenol and papain is rich, the cost of raw materials is low, the raw materials are easy to obtain, in addition, the extraction process is simple, and the production cost is low.

The invention relates to a method for preparing chrome-containing protein filling and retanning agent by using chrome shavings as raw materials. In the chrome-containing protein filling and retanning agent prepared by the prior art, the collagen content in an active material is low. The chrome-containing protein filling and retanning agent prepared by the prior art cannot overcome the off shade drawback of ethenyl resin tanning agents effectively. The preparation method comprises the steps of preparing leather residue-chrome shaving hydrolysis solution and preparing chrome-containing protein filling and retanning agent for leather. In the invention, a process for hydrolyzing chrome shavings without removing chrome is adopted, so a collagen extraction process is simpler and easy in operation; and the obtained chrome shaving hydrolysis solution has high collagen content, and the prepared chrome-containing protein filling, retanning agent can overcome the off shade drawback caused by single ethenyl resin and improve the selective filling of the ethenyl resin, the existing Cr<3+> ions can improve the tanning effect of the ethenyl resin, and at the same time, a chrome pollution problem is solved.

The invention relates to a process method for separating and extracting straw lignin by using eutectic ionic liquid. The method comprises the following steps of: adding a straw powder raw material and eutectic ionic liquid into a normal pressure reactor according to a certain mass ratio, performing mixing and stirring for reaction at 110-160 DEG C for a certain period of time, then regulating the pH value of the mixture to about 11 by using an alkali, performing high-speed centrifugation, and keeping an upper layer solution; and then regulating the pH value of the solution to be less than 3 by using an acid, performing centrifugation again, then washing and centrifuging a deposit by using distilled water until the supernatant is neutral, and performing freeze drying on the final deposit to obtain lignin. The method has the characteristics that the eutectic ionic liquid is prepared under a very mild condition by using low-price and nontoxic choline chloride and an H-bond donor as raw materials, and is used for separating and extracting lignin from rice straws, the raw materials are easy to obtain, the price is low, the process is simple, the condition is mild, the reaction is easy to control, and the energy consumption can be effectively reduced.

The invention relates to a passive sampling device for persistent organic pollutants (POPs) in air. The passive sampling device comprises a stainless barrel, wherein top covers are arranged on upper and lower ends of the stainless barrel; sampling fillers are arranged in the stainless barrel; low-density polyethylene films and stainless outer rings are arranged on the top covers; each low-density polyethylene film is fixed on each stainless outer ring; and the thickness of each low-density polyethylene film is 65 to 90mu m. The POPs in the air or waste air can freely pass through the film, particulate matters in the environment can be prevented from being contacted with the fillers, and gaseous POPs in the ambient air can be acquired, so that the subsequent extraction and purification of a sample are simplified; each low-density polyethylene film is fixed to each top cover, so that the passive sampling device has the advantages that the gas flow volume is larger, and the sampling speed is quicker compared with an X-ray diffraction (XRD) resin sampler; due to the sealed device, the influence of natural environment on the device is small; and the device is not required to be arranged in a special device compared with the conventional polyurethane foam (PUF) passive sampling device and the conventional semi-permeable membrane device (SPMD) sampler, so that the device can be simply and conveniently used.

The invention belongs to the technical field of microbes, and in particular relates to an antimicrobial compound and a fermenting preparation method and application thereof. The molecular weight of the antimicrobial compound of the invention is 445, the molecular formula of the antimicrobial compound is C16H23N5O10, the chemical name of the antimicrobial compound is 1-uracil-4-sarkosyl-serylamido-1,4-dideoxy-beta-D-glucopyranosyl acid, and the antimicrobial compound belongs to uracil hexose dipeptide and is named xin'aomycin. The preparation method adopts actinomycetes Streptomyces noursei and genetically improved strains thereof, and comprises the following steps: culturing in a primary liquid culture medium as a seed liquid; inoculating the cultured seed liquid to a secondary liquid culture medium B for culturing, and carrying out fed-batch fermentation culture of fed-batch liquid C; and after fermentation is finished, separating and purifying the structural compound from the fermentation culture liquid. The antimicrobial compound is used for manufacturing pesticide or medicine products. The invention has the characteristics of high yield, simple process and easy industrialization.

The invention relates to a method for extracting chitin from shrimp and crab shells by using eutectic ionic liquid. The method comprises the following steps: clearing the shrimp and crab shells, soaking in ethanol, cleaning, drying, crushing and the like to obtain shrimp and crab shell powder; mixing choline chloride and thiourea according to a certain proportion, adding into a reaction container, heating in a water bath, and stirring to obtain the eutectic ionic liquid; adding the shrimp and crab shell powder into the eutectic ionic liquid according to a certain mass ratio, heating, stirring and reacting for a certain period of time, taking water as a reverse-phase solvent, centrifugating, separating and drying to obtain the chitin. The method has the characteristics as follows: by virtue of a synergistic effect of the cheap, non-toxic and easily-prepared eutectic ionic liquid prepared from the choline chloride and the thiourea, the solvent and a catalyst, the chitin in the shrimp and crab shells is separated and extracted at an atmospheric pressure, so that the method has the characteristics of a mild treatment condition, a simple extraction process, and high easiness in controlling the reaction; meanwhile, the eutectic ionic liquid is recyclable, so that environment pollution and energy consumption are reduced.

The invention relates to the application of a gene engineering technology in increasing an antibiotic component, in particular to a gene series technology for increasing the main component content ofgene engineering isovaleryl selectomycin. The gene series technology comprises the following steps: connecting ist genes which are closely related to selectomycin isovaleryl acylation in Beta selectomycin gene engineering bacteria in series; increasing the bacterial isovaleryl acylation generating capability by increasing gene dosage; using a promoter erythrocin resistance gene erm promoter sequence with strong promotion activity to replace the original ist gene promoter sequence so as to increase the expression of the series ist genes, and improve the proportion of isovaleryl selectomycin main components of the gene engineering bacteria from a headstream.

The invention discloses mesona chinensis benth series drinks and a preparation method thereof, wherein the method takes mesona chinensis benth as main material to prepare mesona chinensis benth herbal drink, mesona chinensis benth gelatine powder, hot grass jelly electuary and immortal grass tea with milk through the processes of boiling extraction, concentration and the like. Compared with the existing mesona chinensis benth drinks, the product has the effects of cooling, lowering fire and relieving restlessness, has simple and convenient carrying, convenient drinking, and simple ingredients without incompatibility, reduces precipitation and impurities, and retains the special flavour of vegetable material as much as possible, and also has lower cost, exact efficacy, simple production process and easy operation and control, thus ensuring the product quality and being applicable to large-scale production.

The invention discloses a liquid fermentation method for producing natto kinase in high yield, which comprises a fermentation formula and fermentation conditions. In the method, a natto solid fermentation high-yield strain is selected and subjected to mutation and domestication so as to screen a high-yield strain of natto kinase; and the high-yield strain is inoculated into a liquid nutrient medium for fermentation, wherein the enzyme activity can reach 1,400IU / mL under liquid fermentation of a shake flask. Enzymatic hydrolysate of bean pulp is adopted as a nitrogen source of pilot plant test fermentation, and in a 10L fermentation tank, the fermentation unit can reach 2,650IU / mL. Due to the technical scheme, the yield of the prepared fermentation product natto kinase is high, the enzyme activity is high, the raw materials are saved, the energy is saved, the used raw materials are convenient to obtain and rich, the cost is low, and the extraction technology is easy and practicable.

The invention provides a collagen gel and a preparation method thereof. The collagen gel comprises collagen and an antibacterial agent, wherein the antibacterial agent is at least one of chitosan and nano silver. The collagen gel also comprises a preservative, a substrate, a dispersing agent, a film-forming agent and water; and 1g of gel comprises 0.5 to 8.0mg of collagen, 0.001 to 15mg of antibacterial agent, 1 to 50mg of preservative, 10 to 50mg of matrix, 50 to 100mg of dispersing agent, 1 to 10mg of film-forming agent, and the balance of water. The method for preparing the collagen gel comprises the following steps of: performing dispersion and dissolution on the substrate by adopting the dispersing agent under ten-thousand grade clean environment; adding the dispersing agent, the substrate, the preservative, the antibacterial agent, and collagen solution into a vacuum emulsified homogenizer one by one; after fully mixing, standing and defoaming, filling the product. The collagen gel is applied to various open wounds and infected wounds, lightens hyperpigmentation, has good effect of accelerating wound healing, and can maintain long-term anti-bacteria function.

The invention discloses a method to manufacturing high purity chemocholic acid from fowl bile that belongs to refinement chemical and medicine field. It includes the following steps: a. saponification reaction: mixing the fowl bile and sodium hydroxide and reacting for 20-30 hours under the certain temperature; b. neutral reaction: adding dilute hydrochloric acid after saponification reaction and gaining raw cholic acid after filtering; c. column chromatography: adding dilute sodium hydroxide into raw cholic acid and injecting into chromatography column, uniting the eluent; super filtering: taking super filtering by super filtering film; e. gaining chemocholic acid that the purity is over 96% after adding dilute acid, filtering and drying. The invention has simple technology, high purity, low cost and environment protection.

This invention involves the surplus activation and pollutes proteins as the application of the foam well drilling liquid and prepares the method, utilize surplus active mud person who foam of protein is it foam the pharmaceutical as foam well drilling to use it for, it is to use active mud the impractical law or alkali law that it prepares the method, yes temperature is 110-148 deg.C hydrolysis one hour under the terms, it is appear impurity win protein liquid, and then go protein liquid after the impurity transfer pH value to filter, to a little becoming used as preparing hydrolysising proteins of foam well drilling liquid alkalinly promptly, and then make and foam pharmaceutical and dispose the foam well drilling liquid with the protein hydrolysats. Invention this utilize surplus active mud characteristic of foaming of protein, hydrolysis active mud protein regard as foam well drilling liquid foam the pharmaceutical, its bubble is of good performance, the foaming amount is big, can produce a large number of foam after contacting the gas.

A process for extracting the codonopsinose used to prepare the codonopsinose medicines in the form of infusion, capsule, syrup, or oral liquid includes cutting the pilose asiabell root, decocting 2-3 times, collecting filtered decoction, vacuum concentrating, cooling, centrifugal separation, drying the deposit, and pulverizing.

The invention relates to a method for detecting microplastics in an organism. The method comprises steps as follows: S110, digestion: an organism sample is soaked in nitric acid with the concentrationof 50%-70% at the room temperature for 6-18 h, heated to 60-95 DEG C and maintained for 1-4 h, and a digested organism sample is obtained; S120, degreasing: the organism sample obtained in S110 is washed with a surfactant at the temperature of 60-95 DEGC and subjected to suction filtration while hot, and degreased microplastics are obtained; S130, the degreased microplastics obtained in S120 arewashed with water at the temperature of 60-95 DEGC and subjected to suction filtration, and the cleaned microplastics are obtained. By means of the method, qualitative and quantitative detection can be rapidly and accurately performed on the microplastics entering the organism, the purpose of enrichment and distribution of the microplastics in the organism is achieved, and a practical tool is provided for environment monitoring.

The invention relates to a production method for synthesizing C8:0 / C10:0 / C12:0 / C14:0 medium-chain fatty acid and ethyl ester thereof by using engineering Escherichia coli. The production method comprises the following steps of: cloning thioesterase genes with different specificities chFatB / ucFatB / ccFatB in cuphea / laurel / camphorwood into an expression vector pETDuet-1 to obtain a corresponding recombinant plasmid; transferring the recombinant plasmid into Escherichia coli BL21, performing isopropyl-beta-d-thiogalactoside (IPDT) induction expression, and specifically hydrolyzing acyl carrier protein (ACP) with specific chain length by using the expressed thioesterase to obtain medium-chain fatty acid; cloning acyltransferase gene (atfA) in acinetobacter into expression vectors pETDuet-chFatB / pETDuet-ucFatB / pETDuet-ccFatB to obtain corresponding recombinant plasmids; and transferring the recombinant plasmids into the Escherichia coli BL21, performing IPTG induction expression, performing acyl transfer reaction on exogenously fed ethanol and acyl-CoA formed by the medium-chain fatty acid intracellularly by using the co-expressed acyltransferase to obtain fatty acid ethyl ester.

The invention discloses an extracting process of dendrobium candidum polysaccharides. The process comprises the following steps of: mixing dendrobium candidum and water according to a ratio of liquid to material of 51-52 ml of water per 1 g of dendrobium candidum, and extracting at 92.5 DEG C to 93 DEG C for 2.5-2.6 hours to obtain dendrobium candidum polysaccharide extract. The invention further provides a method for optimizing the extracting process of the dendrobium candidum polysaccharides by a response surface method, and the method comprises the following steps: setting three variables: a ratio of liquid to material of the dendrobium candidum to the water, extracting temperature and extracting time; and optimizing by utilizing a design expert software according to a Box-Behnken design principle. By adopting the extracting process provided by the invention, the yield of the dendrobium candidum polysaccharides can be improved, the consumption of water resources is reduced, and the generation and emission of sewage in a production process can be decreased.

The present invention discloses one kind of glabrous greenbrier total saponin extract and its preparation process and use. The extraction process includes the following steps: 1. percolation extracting glabrous greenbrier with water solution of alcohol and merging the extracted liquid; 2. concentrating the extracted liquid while recovering alcohol; 3. extracting with ethyl acetate for several times and merging the extracted liquid; and 4. drying the extracted liquid to obtain the glabrous greenbrier total saponin extract while recovering ethyl acetate. The glabrous greenbrier total saponin extract contains glabrous greenbrier total saponin in 50-80 wt%, and astilbin in 20-40 wt%. The present invention features the simple technological process, and high glabrous greenbrier total saponin and effective component content in the extract. The glabrous greenbrier total saponin extract has obvious inhibition on various kinds of inflammation reaction and immune response, and may be used in preparing corresponding medicine.

The invention discloses a preparing method and application of natural dye of spatholobus stem, which comprises the following steps: grinding spatholobus stem; immersing in the sodium carbonate solution with pH value at 8-10 under 95-100 deg. c for 60-90min; filtering to obtain the primary liquid of natural dye; condensing the primary liquid; solidifying; grinding; obtaining the powder-shaped natural dye.

The invention relates to a method for extracting a silk protein. At present, a method for extracting the silk protein, in which the extraction condition is mild and which facilitates reducing the influence of the extraction condition on the structure and performance of the silk protein, is not provided. The method comprises the following steps of: placing the raw materials for preparing the silk protein in a CaCl2 aqueous solution at the temperature of between 95 and 100 DEG C, and dissolving until an undissolved part is jelly to obtain a dissolution treatment solution; filtering the dissolution treatment solution by using filter paper to obtain a silk protein / CaCl2 mixed aqueous solution and the undissolved jelly; washing the undissolved jelly to obtain silk protein colloid; and / or placing the silk protein / CaCl2 mixed aqueous solution in a dialysis bag and dialyzing and purifying to obtain a silk protein solution; and processing the silk protein colloid and / or the silk protein solution to obtain a finished product silk protein. According to the method, the extraction condition is mild, the extraction efficiency of the silk protein is improved conveniently, the waste of the silk protein resources is reduced, and the influence of the extraction condition on the structure and performance on the silk protein is reduced.

The invention discloses an extract of agrimony total flavonoids and an extraction and preparation method thereof; the total amount of ketosteroid compounds accounts for 25 percent to 100 percent of the total extraction matter. The invention takes agrimony as raw material and organic solvent whether containing or not containing water as extraction solvent. The extraction procedure is as following: extracting, leaching with solvent and adsorbing resin column to make purification process or using inorganic acid to regulate the PH value of concentrated solution to be no higher than 3, and then obtaining the extraction matter of agrimony after processing. Experiments prove that the extraction matter of agrimony total flavonoids has functions of antioxidation, anti-myocardial ischemia and reducing blood lipid. The extraction matter of agrimony total flavonoids can be used as food preservative and health product additive; the extraction matter of agrimony total flavonoids, carriers acceptable in pharmacy or excipient are made into various pharmaceutical dosage forms for anti-myocardial ischemia and reducing blood lipid. The invention has the advantages of simple technics and good treatment effect and can be widely used for curing or preventing coronary heart disease, hyperlipoidemia, high blood pressure or preventing cardio-cerebrovascular diseases such as atherosclerosis, etc.

The invention provides a method for producing L-ornithine by microbial fermentation, namely, using Corynebacterium glutamate to obtain the strains of CS-189(cit<(-)>+SG<r>) by chemical treatment, and obtain the L-ornithine hydrochloride by fermentation, culture solution micro-filtration by a ceramic membrane, ion exchange resin and extraction by a hydrothermal crystallization method with decompression concentration. The strains used in the method are auxotrophy resistant to sulfaguanidine, which significantly enhances acid yield by fermentation. Meanwhile, aiming at the problem that the solubility of the L-ornithine hydrochloride in water is extremely difficult, the hydrothermal crystallization method is adopted to obtain better extraction rate under the condition that flammable and explosive alcohol is not used. The method simplifies processes and reduces extraction cost.

The invention provides a rhodopseudomonas palustris strain, a bacterial agent, a preparation method of the bacterial agent, extracelluar protein as well as an extraction method and application of the extracelluar protein. The rhodopseudomonas palustris strain is rhodopseudomonas palustris LY-6 which is preserved in China Center for Type Culture Collection; the preservation number is CCTCCM2014525; the bacterial agent is prepared by activating rhodopseudomonas palustris strain, culturing seeds, producing, culturing and centrifuging. The invention also provides extracelluar protein which is extracted from the rhodopseudomonas palustris bacterial agent; the extraction method comprises the steps of precipitating and salting ammonium sulfate out, centrifuging, desalting by SephadexG-25 and carrying out SephadexG-25 column chromatography isolation; an extraction method of the extracelluar protein is simple, short in process and high in extraction efficiency; the extracelluar protein has in-vitro inactivation on tobacco mosaic virus.

The invention relates to multifunctional face cream and a preparation method thereof. The face cream is made from and combines Sargassum fusiforme extract obtained by an ultrasonic extraction method and other components beneficial for skin care. The Sargassum fusiforme extract contains polysaccharides of Sargassum fusiforme, vitamin E, vitamin C, SOD and other skin-caring components, and has moisturizing, antioxidant and antiaging effects. The face cream has multiple functions, and the extraction of the Sargassum fusiforme extract is simple. The raw materials of the face cream are easy to obtain and the preparation method thereof is simple.