Method for producing L-ornithine by microorganism fermentation
A technology of microbial fermentation and ornithine, applied in the field of microbial fermentation technology, can solve the problems of difficulty in large-scale production, high cost, and limiting the wide application of L-Orn.
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[0076] The present invention will be further described below by the examples, but the examples do not limit the protection scope of the present invention.
[0077] 1. Strain selection
[0078] (1) Strains
[0079] The starting strain is Corynebaetenom glutamicam preserved in this laboratory
[0080] (2) Culture medium and mutagenesis method
[0081] 1. The composition of the complete medium (A)
[0082] 1% beef extract; 1% peptone; 0.5% yeast extract; 0.5% NaCl; PH7.0 (PH6.4-8.0 test paper produced by Shanghai Reagent No. 4 Factory); add 2.0% agar powder when preparing solid medium.
[0083] 2. The composition of basic medium (B)
[0084] Glucose: 1%; (NH 4 ) 2 SO 4 : 0.3%; KH 2 PO 4 : 0.1%; K 2 HPO 4 ·3H 2 O: 0.2%; MgSO 4 ·7H 2 O: 0.05%; FeSO 4 ·7H 2 O: 0.002%; MgSO 4 0.002%, V B1 . HCl 200 μg / L, V H 50 μg / L, PH7.0~7.2 (adjusted with NaOH); agar powder 1.6%.
[0085] 3. Preparation of 1 / 30M phosphate buffer solution (PH7.0)
[0086] Liquid A: Na2 HPO 4 ...
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