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Method for extracting silk protein

A technology of silk protein and extraction method, applied in the field of silk protein processing technology, can solve the problems of easy degradation of silk protein, severe degradation of degumming solution, complicated extraction process, etc., and achieves wide application range, high extraction efficiency and simple extraction process. Effect

Inactive Publication Date: 2012-07-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These traditional silk protein extraction methods have the disadvantages that silk protein is easy to degrade (especially the extraction of sericin), the extraction process is complicated (two steps are required to obtain silk fibroin), waste of resources (the degumming solution in the process of silk fibroin extraction degrades Powerful, difficult to purify and utilize) and other shortcomings
[0005] At present, there is no silk protein extraction method with simple process, mild extraction conditions, which is beneficial to improve the extraction efficiency of silk protein, reduce the waste of silk protein resources, and reduce the influence of extraction conditions on the structure and performance of silk protein.

Method used

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  • Method for extracting silk protein

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Embodiment 1

[0024] The method for extracting silk protein in this embodiment includes the following steps in sequence.

[0025] (1) Weighing 0.5g of cocoon layer of silkworm cocoon, peeling off the cocoon layer of silkworm cocoon into thin cocoon layer to obtain silk protein raw material. The present invention can peel off the cocoon layer of silkworm cocoon into thin layers or cut the cocoon layer of silkworm cocoon into pieces or cut the cocoon silk of silkworm cocoon into short fibers to obtain the silk protein raw material, wherein the used cocoon silk includes cocoon layer silk, cocoon silk Cloth and chrysalis lining.

[0026] (2) Put the silk protein raw material prepared in step (1) in CaCl with a mass concentration of 50% (w / w) at a temperature of 98°C 2 dissolved in aqueous solution, CaCl 2 The aqueous solution is kept in a slightly boiling state, the bath ratio is 1:25, and the dissolving treatment is carried out for about 5 minutes until the undissolved part of the fibroin be...

Embodiment 2

[0031] see figure 1 , the method for extracting silk protein in this embodiment includes the following steps in turn.

[0032] (1) Weighing 0.5g of cocoon layer of silkworm cocoon, peeling off the cocoon layer of silkworm cocoon into thin layers to obtain silk protein raw material.

[0033] (2) Put the silk protein raw material prepared in step (1) in CaCl at a temperature of 96°C 2 Dissolve in an aqueous solution, the mass concentration of calcium chloride is 50% (w / w), the bath ratio is 1:25, and the dissolving treatment is carried out for about 5 minutes until the undissolved part of the silk protein raw material becomes jelly, thereby obtaining the dissolving treatment liquid .

[0034] (3) Filter the solution prepared in step (2) with filter paper, rinse with a small amount of pure water, and separate by filtration to obtain silk protein / CaCl 2 Mix the aqueous solution and unsoled gel.

[0035] (4), the silk protein / CaCl in step (3) 2 The mixed aqueous solution is pl...

Embodiment 3

[0038] The method for extracting silk protein in this embodiment includes the following steps in sequence.

[0039] (1) Weighing 1g of cocoon layer of silkworm cocoon, peeling the cocoon layer of silkworm cocoon into thin layers to obtain silk protein raw material.

[0040] (2) Put the silk protein raw material prepared in step (1) in CaCl at a temperature of 100°C 2 Dissolve in an aqueous solution for about 5 minutes until the undissolved part of the silk protein raw material becomes jelly, thereby obtaining a solution for dissolving. The mass concentration of calcium chloride used in this example is 50% (w / w), and the bath ratio is 1:25.

[0041] (3) Filter the solution prepared in step (2) with filter paper, rinse with a small amount of pure water, and separate by filtration to obtain silk protein / CaCl 2 Mix the aqueous solution and unsoled gel.

[0042] (4) Washing the unsolvated substance in step (3) with water for several times to remove the adhered calcium chloride m...

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Abstract

The invention relates to a method for extracting a silk protein. At present, a method for extracting the silk protein, in which the extraction condition is mild and which facilitates reducing the influence of the extraction condition on the structure and performance of the silk protein, is not provided. The method comprises the following steps of: placing the raw materials for preparing the silk protein in a CaCl2 aqueous solution at the temperature of between 95 and 100 DEG C, and dissolving until an undissolved part is jelly to obtain a dissolution treatment solution; filtering the dissolution treatment solution by using filter paper to obtain a silk protein / CaCl2 mixed aqueous solution and the undissolved jelly; washing the undissolved jelly to obtain silk protein colloid; and / or placing the silk protein / CaCl2 mixed aqueous solution in a dialysis bag and dialyzing and purifying to obtain a silk protein solution; and processing the silk protein colloid and / or the silk protein solution to obtain a finished product silk protein. According to the method, the extraction condition is mild, the extraction efficiency of the silk protein is improved conveniently, the waste of the silk protein resources is reduced, and the influence of the extraction condition on the structure and performance on the silk protein is reduced.

Description

technical field [0001] The invention relates to a method for extracting silk protein, which belongs to a processing technology of silk protein. Background technique [0002] Silk is mainly composed of two proteins, sericin and silk fibroin, collectively called silk protein. Sericin surrounds the surface layer of silk fiber and acts as an adhesive. Sericin and silk fibroin have different structures and properties: sericin is a globular protein, its secondary structure is mainly random coils, and its stability is poor. It is soluble in solutions such as hot water, acid, alkali, protease and low-concentration sodium carbonate. ;Silk fibroin is a fibrous protein, containing a large number of crystal structures, stable performance, soluble in high concentration calcium chloride (CaCl 2 ), calcium chloride ethanol water (CaCl 2 / CH 3 CH 2 OH / H 2 O) mixed solution and lithium bromide (LiBr) and other solutions. Therefore, according to the solubility difference of silk protei...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14
Inventor 张海萍朱良均杨明英闵思佳施李杨
Owner ZHEJIANG UNIV
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