380 results about "Sericin" patented technology

There is provided a novel wound dressing material which has biocompatibility and infection controllability as essential properties required for such a material, especially excellent flexibility and water absorption properties, thereby accelerating smooth regeneration of a skin defect without stripping off the regenerating skin while removing the material from the skin. A healing agent is added to the wound dressing material which comprises an amorphous film of a crystallinity below 10% and contains fibroin and sericin as a main component.

The present invention relates to a membrane for guided bone tissue regeneration and, more particularly, to a membrane for guided bone tissue regeneration having a structure that silk fibroin nanofibers obtained by removing sericin from silk fibers are formed as a nonwoven, and a manufacturing method thereof. A membrane for guided bone tissue regeneration according to the present invention has a predetermined strength, biocompatibility, and biodegradability, and may maintain a sustained drug release system, when drugs are added in the manufacturing process. Additionally, a membrane for guided bone tissue regeneration according to the present invention may be modified corresponding to the condition of usage, because a thickness of the membrane may be adjusted by controlling fineness of nanofibers, compactness of nanofibers, and pore size of a multiporous structure may be adjusted, in a nonwoven manufacturing process. A nanofibrous membrane for guided bone tissue regeneration according to the present invention is manufactured by freezing rapidly, drying a silk fibroin solution obtained by removing sericin from silk fibers, and by electrospinning after dissolving the dried silk fibroin in an electrospinning solvent. The membrane according to the present invention has excellent adhesion and air permeability, and is thereby effective in regeneration of damaged periodontal tissues.

A process for producing non-woven silk fiber fabrics comprises the following steps: a) obtaining silk fibroin, for example either from silk cocoons, or silk textiles or waste silk; b) removing the sericin layer covering the silk fibroin fibers, when present; c) breaking the disulfide bonds between heavy (350 kDa) and light (27 kDa) chains of silk fibroin in order to obtain the production of chain fragments which serve as a specific cellular recognition sites promoting the attachment and growth of cells, d) homogenising of the material resulting from step c).

The invention discloses a preparation method of sericin hydrogel, the method is as follows: first, weighting domestic silkworm fibroin deletion form mutation variety silkworm cocoon for LiBr or LiCl extraction and dialysis and purification to obtain a non-degradable sericin water solution with a mass percentage concentration of 0.1-4%; concentrating the sericin water solution to 1.5-10%, adding a cross-linking agent to the concentrated sericin water solution (wherein 2-500muL of the cross-linking agent is added into each L of the sericin water solution), fully mixing, and placing at 4-45 DEG C for 5 seconds to 36 hours to obtain the sericin hydrogel. The sericin hydrogel has biological activity and multiple functions, can be used as carrying growth factors, drugs and cell carriers treatment, and can be applied in repair of a variety of soft tissue injuries, including but not limited to skin damages, muscle damages, injuries of blood vessel, nerve injuries, myocardial damages, and the like, and treatment of diseases.

The invention discloses a fruity probiotic yogurt slice containing antifreeze sericin peptide. The raw materials for preparing the fruity probiotic yogurt slice comprise 10-14 parts of skimmed milk powder, 2-5 parts of sucrose, 1-3 parts of sericin peptide, 1-4 parts of fruit material, 0.1-0.6 part of lyophilized active probiotics and 0.1-0.4 part of magnesium stearate. The invention further discloses a method for preparing the product which is the fruity probiotic yogurt slice. The method mainly comprises the following steps of: preparing a skimmed-milk food-grade culture medium, fermenting the probiotics, adding the antifreeze sericin peptide, freezing and drying fermented milk in vacuum, and pressing powder into slices. As the fruity probiotic yogurt slice contains the antifreeze sericin peptide and the lyophilized fruit powder which is rich in the vitamin B group, the number of the probiotics in the fruity probiotic yogurt slice can be increased, the retention time of the activity of the probiotics can be prolonged, and the taste, flavor and nutritional and healthcare values of the fruity probiotic yogurt slice can be improved. The fruity probiotic yogurt slice can serve as a functional food for supplementing the vitamin B group, improving the immunity and promoting the intestinal health.

Preparation of sponge-like porous materials of silk protein is carried out by converting its conformation with denaturant at low temperature to have frozen phase separation to obtain the product. Its parameters can be regulated by controlling concentration of the silk protein, amount of denaturant or its characteristics, freezing time or post-treatment in porosity, pore size, and mechanical performance. Neither cross-linker nor pore-generating agent nor surfactant nor organic solvent with strong toxicity is used. It is produced in a simple process, and has a uniform texture, high porosity up to 98%, good toughness and elasticity and reliability, water absorbing rate up to 5000%, elastic recovery rate 100% and press deformation >90%.

The invention discloses a low-temperature cocoon cooking process for a mulberry cocoon with little sericin, which comprises a step of soaking dry mulberry cocoon with little sericin in water at the temperature of 35 DEG C under vacuum, wherein the water temperature of the I soaking section on an upper groove of a cocoon cooking machine is 35 DEG C; the water temperature of the II soaking section is 45 DEG C; the cooking temperature is 80 DEG C; the temperatures of a middle section, a vibrating section and a statically cooking section on a cooking part are 50 DEG C, 40 DEG C and 30 DEG C respectively; the temperature of an outlet part is 30 DEG C; the cocoon cooking time is 14 minutes; and the difference between the reelability percentage of the low-temperature cocoon cooking process and the reelability percentage of a high-temperature cocoon cooking process is no more than 5 percent. Matched with the popularization of new products of the mulberry cocoon with little sericin, the low-temperature cocoon cooking process realizes energy conservation and emission reduction in a reeling process and has direct economic benefit and wide social benefit for creating an energy-saving and emission-reducing 'low-carbon' society.

The invention relates to a silk producing method which comprises the steps of: high-temperature direct steaming: processing silkworm cocoons with saturated steam in an direct steaming chamber or a direct steaming device, wherein the direct steaming temperature is 100 DEG C, the direct steaming time is 8 to 10 minutes; standing: standing the directly-steamed silkworm cocoons at a normal temperature for 18 to 26 hours; vacuum infiltration: infiltrating the stood silkworm cocoons by utilizing a vacuum infiltration method to subject the silkworm cocoons to absorbing water, wherein the infiltration water temperature is 35 DEG C to 40 DEG C, the infiltration time is 5 to 10 minutes, and the vacuum degree is 0.05 to 0.1 Mpa (megapascal); cocoon cooking: cooking the silkworm cocoons subjected to vacuum infiltration in a cocoon cooking device; silk reeling: dissociating cocoon filaments of the cooked silkworm cocoons at a temperature of 40 DEG C, and utilizing sericin to glue and connect the cocoon filaments to form raw silk; and post-reeling which includes steps of roughness removal, small reel silk sheet balancing, small reel vacuum moisture absorption, rereeling, large reel silk sheet balancing, arranging and the like. The silk producing method enables the neatness of the raw silk to be relatively high by utilizing high-temperature direct steaming and vacuum infiltration.

The invention discloses a natural rubber liquid slurry containing chitosan / sericin and a preparation method thereof, and belongs to the field of high polymer chemistry. The invention is characterized in that the preparation method comprises the following steps: respectively preparing a chitosan water solution and a sericin water solution, mixing the two solution, and regulating the pH value of the mixture to 9-11 by using ammonia water and a citric acid solution; and adding the obtained chitosan / sericin mixed solution to a natural latex slurry, adding a laurate surfactant, and mechanically stirring the mixture uniformly to obtain the natural rubber liquid slurry containing chitosan / sericin. The invention has the advantages that the chitosan has the functions of adsorbing water soluble proteins in the latex, inhibiting bacteria and viruses from breeding, and the like; and the sericin has the functions of oxidation resistance, allergy resistance, hydrophilia, oil resistance and the like, thereby enhancing the emulsion phase stability and the preservation effect of the natural latex. In particular, the product obtained by using the method overcomes the defects of short service life, easy aging and sticking, poor oil resistance, initiation of protein allergy and the like in the traditional natural latex product.

The invention relates to a method for producing mulberry silk quilt which can be washed via water. Wherein, it comprises that degelatinizing the pod and using cotton machie to prepare cotton sheets, arranging cotton sheet in normal temperature to expand sericin; degelatinizing the cottom sheet; cooling, poaching, and neutralizing, adding flexible agent, filtering; adding water-resistant agent, drying at 100-150Deg. C for 30-60mins; tensioning the dried cotton sheet, to expand the cotton sheet, and packing it into coat.

The invention relates to silk property-variable non-woven cotton with sericin and relative application. Said silk property-variable non-woven cotton via selection-modifying-blanching, and washing-carding-needling processes can be made into the silk property-variable non-woven cotton which will not lose silk. Said non-woven cotton can recover water-solubility and biological activity via protein recover treatment to supply the health function of silk protein, and apply the medical, hairdressing and daily consumption areas. Since said invention can keep a lot of sericin protein, therefore, it has lower cost, better fiber flexibility and thermal insulation than traditional silk non-woven cotton, and it can avoid de-gumming process to reduce environment pollution with better economic, social and zoology benefits

The invention discloses a cleaning cocoon cooking method. The method comprises the processes of: external vacuum infiltration, cooking by an automatic cooker, cocoon steaming, and adjustment, wherein a reelability agent originally used for cocoon cooking replaces a penetrating agent for penetrating to be used in the external vacuum infiltration cocoon cooking process; in the condition with 30-50 DEG C water temperature, the reelability agent can only have infiltration function with water to be adsorbed between cocoon layers and on the surface of a cocoon filament; and in the cocoon cooking process and in the condition with 60-80 DEG C water temperature, the reelability agent can promote the cocoon filament sericin dissolution to reduce adhesion between cocoon filaments for the smooth dissociation of the cocoon filaments, so that the reelability agent has the reelability function in the cocoon cooking process. One chemical preparation achieves two functions, the chemical preparation is less and easily controlled in dose, process is simple, operation is convenient, and production cost is low.

Provided is an artificial eardrum using silk protein and a method of fabricating the same. The artificial eardrum is fabricated in the form of a silk membrane by desalinating and drying silk protein (or silk fibroin) or a silk protein complex solution obtained after removal of sericin from a silkworm cocoon or silk fiber. Thus, regeneration of an eardrum perforated due to disease or a sudden accident is stimulated, a boundary of the regenerated eardrum is clean and biocompatibility and transparency are increased. In addition, the artificial eardrum may be fabricated using the silk protein or silk protein complex solution obtained from a silkworm cocoon alone or mixed with collagen, alginic acid, PEG or pluronic 127.

A gel film of polyvinyl alcohol and sericin mixture with uniform microstructure, high mechanical performance and high bioactivity is prepared through respectively preparing polyvinyl alcohol solution and sericin solution, proportional mixing, stirring, pouring in a container with flat bottom, sealing, and freezing-thawing several times.

The invention relates to a sericin-based semi-interpenetrating temperature-sensitive nano-composite hydro-gel and a preparation method thereof. The hydro-gel comprises a sericin polypeptide chain part, an inorganic nano-soapstone particle part and a polymer formed after an alkylacrylamide monomer is polymerized. In the hydro-gel, a semi-interpenetrating network technology is mainly adopted, and by combining the physical crosslinking effect of inorganic nano-soapstone particles, the sericin, the inorganic nano-soapstone particles and in-situ free radical of the alkylacrylamide monomer are polymerized. The preparation method comprises the following steps of: dispersing the inorganic nano-soapstone particles; preparing a reaction prepolymerization solution; preparing gel; and purifying the gel. The sericin-based semi-interpenetrating temperature-sensitive nano-composite hydro-gel has rich raw material sources, simple preparation method, low cost, high swelling degree and excellent cell compatibility, can be adjusted according to the content of sericin, and has a wide application prospect in artificial skin, artificial cartilage, cell culture stent, bioreactor and other biomedical aspects.

The invention discloses a sericin post-finishing method for cotton fabrics, which comprises the following steps: (a) configuring settling impregnation liquid: dissolving 3-5% of dissolvable sericin powder counted by mass in hot water, and then adding 1-3% of chitosan quaternary ammonium salt, fully stirring and dissolving, and adding 4-6% of cross-linking agent, and (b) completely steeping the cotton fabrics in the settling impregnation liquid at a solution bath ratio of 1:(30-50), steeping for at least 20 minutes, twice steeping and twice rolling, keeping mangle expression more than 80%, anddrying, thereby obtaining the cotton fabrics. The invention develops the post-finishing method special for the cotton fabrics. The cotton fabrics prepared according to the post-finishing method have better washing fastness and anti-bacteria rate; after the cotton fabrics are washed for five times, a dissolving loss rate is about 0.21% and the anti-bacteria rate is 93.8%; the practical use requirement is fully met; and an extraordinary technical effect is obtained.

The invention relates to the technical field of photo-curing hydrogels, and in particular relates to a visible light initiating system in a medical photo-curing hydrogel and a photo-curing method thereof. The visible light initiating system in the medical photo-curing hydrogel contains the following components in part by weight: 0.5-1 part of the first component: photosensitizer, and 0.5-5 parts of the second component: auxiliary initiator; the auxiliary initiator is water-soluble sericin or fibroin powder, the photosensitive synergist is one of iodonium salt, sulfoniumsalt or phosonium salt. The system is good in solubleness in water, and excellent in biocompatibility or biosecurity; and the free radical produced after absorbing 400-700nm of visible light can efficiently initiate the polymerization and curing of the precursor aqueous solution containing unsaturated double bond, thereby preparing the multipurpose medical hydrogel.

The present invention discloses preparation process of skin care silk protein gel cream. The preparation process includes the following steps: adding water soluble sericin or water soluble sericin and silk peptide as gel promoter into water solution of fimbrin through homogeneous mixing; stilling the solution at 0-90deg.c for gelatinizing the solution into translucent silk protein gel with water content of 70-98 %; and homogenizing and emulsifying to form silk protein gel cream. Into the silk protein gel cream, other cosmetics matrix component and supplementary material component may be added as required. The silk protein gel cream has the functions as nutrient additive, humectant and matrix, and may be used in producing high quality natural skin care cosmetics.

Preparation of sponge-like porous materials of silk protein is carried out by converting its conformation with denaturant at low temperature to have frozen phase separation to obtain the product. Its parameters can be regulated by controlling concentration of the silk protein, amount of denaturant or its characteristics, freezing time or post-treatment in porosity, pore size, and mechanical performance. Neither cross-linker nor pore-generating agent nor surfactant nor organic solvent with strong toxicity is used. It is produced in a simple process, and has a uniform texture, high porosity up to 98%, good toughness and elasticity and reliability, water absorbing rate up to 5000%, elastic recovery rate 100% and press deformation >90%.

Provided are an artificial eardrum using silk protein and a method of fabricating the same. The artificial eardrum is fabricated in the form of a silk membrane by desalinating and drying silk protein (or silk fibroin) or a silk protein complex solution obtained after removal of sericin from a silkworm cocoon or silk fiber. Thus, regeneration of an eardrum perforated due to disease or a sudden accident is stimulated, a boundary of the regenerated eardrum is clean and biocompatibility and transparency are increased. In addition, the artificial eardrum may be fabricated using the silk protein or silk protein complex solution obtained from a silkworm cocoon alone or mixed with collagen, alginic acid, PEG or pluronic 127.

A process for removing sericin from silkworm cocoons includes such steps as adding silkworm cocoons into a rotary container, sealing the container, high-temp. and high-pressure treating for a certain, time to strip sericin from silk, suddenly releasing pressure to make the cocoon become puffed silk floss separated from silk and sericin, separating sericin from silk, and dewatering each of them. Its apparatus is composed of the container with exploding membrane on the cover and drier.

The invention discloses a method for processing sericin fiber containing silk. It includes rouging, swelling, solidifying sericin, removing aldehyde group, synthesizing, and drying. Because of using acid liquor, aldehyde group, alkali liquor, and hydrogen peroxide as the main material, the cost is low; it is no pollution. The processed product is accorded with the country standard. The technique is simple, and easy to be industrialization produced. The used acid liquor and solidifying sericin can be reclaimed. The dehydrating aldehyde group solution is discharged after treatment. Thus the production technique is accorded with the environmental protection demand.

The invention discloses a refining process for a tussah cut cocoon. The process is realized through the following steps of: pre-soaking, washing and dehydration, cocoon cooking, washing and dehydration, bleaching, washing and dehydration, softening, dehydration, drying, waste silk opening. The process provided by the invention solves the problems of difficult deoiling, excessive sericin shedding, crispy and breakable silk, undesirable silk floss bulking intensity and fibre breaking strength, low cleanliness and the like in traditional process technologies of tussah cut cocoon shells. According to the process, the product quality can reach and exceed the national industry standards, thereby improving the quality of tussah silk refined waste silk and tussah silk quilt products.

The invention relates to a process for producing a silk quilt by utilizing sericin, which comprises the following steps of: sequentially carrying out vacuum penetration and cocoon cooking on raw material cocoons; then carrying out one-particle hemming to form a yarn sheet; carrying out sericin fixation processing on the yarn sheet to enable the sericin to be fixed; and after carrying out post-treatment, drawing to form a silk quilt inner tube. The process is characterized in that in both the vacuum penetration and cocoon cooking processes, a raw silk softener is added; the yarn sheet is timely dried by infrared light or hot air after one-particle hemming is carried out to form the yarn sheet; before the sericin is fixed, the yarn sheet has sericin content of 15 percent to 26 percent; and the sericin is fixed by a cross-linking agent. According to the invention, the sericin can be reserved to the greatest degree; and denatured proteins of the silk quilt can be reduced, and silk fibers containing the sericin not only are soft, but also have stable performance.

The invention belongs to the technical field of biological medicine materials, and discloses a tumor targeted sericin micelle, and a preparation method and an application thereof. According to the method provided by the invention, with water soluble natural protein sericin having good biocompatibility and biodegradability as a raw material, an amphiphilic sericin derivative containing a hydrophobic cholesterol group is synthesized under a room temperature condition, folic acid is further coupled by a chemical reaction to prepare a targeted amphiphilic sericin derivative, and a drug-loaded nanometer micelle can be formed with a hydrophobic drug IR780 in an aqueous solution by means of the self assembly. The nanometer micelle system can effectively improve the stability of the drug in the blood circulation and reduce the toxic and side effects of the drug, thereby having a very good research value and application prospect in the preparation of tumor diagnostic agents or treatment drugs.

The invention relates to wild silk surgical operation suture thread and the preparation method. The wild silk yarn surgical operation suture thread adopts wild silk woven or twisting thread as thread rod and the surface is covered by wild sericin. The preparation method comprises the following steps: (1) place and immerse the wild silk into the aqueous solution, which comprises 0.1-0.3wt% sodium salt, 0.1-0.2wt% surface-active agent and 0.01-0.03wt% penetrating agent for 15 to 20 minutes; (2) after fish out and dry the wild silk, use machine to weave and twist the wild silk into the thread rod with required specification; (3) apply the wild sericin on the thread rod; and (4) heat up the silk for forming and the heating temperature should be controlled between 80 to 100 degrees centigrade. The suture thread has the advantages of high strength, smooth thread, easy knotting, good absorbing performance, good pancreatic juice and bile tolerance, easy dialyneury and capability of promotion of nerve regeneration.

This invention discloses one kind of silk textile fiber and its fabric fining come unglued method. Front it uses the low temperature plasma technology carries on to the raw silk textile fiber and its the fabric processes, substitutes the conventional fining come unglued center's pretreatment, initially practices working procedure, then the direct process duplicate practices, achieves to the silk textile fiber and its the fabric concise come unglued's goal. Because the low temperature plasma physical ability sericin the macro-molecule carries on to the raw silk textile fiber and its fabric surface shells and splatters sculpture, causes its stopped chain or sculpture dehiscence, loose, duplicate causes to practice the fining medicinal preparation to be able fast to seep, bulked, degrades (hydrolisis or enzymolysis) sericin, therefore, has the craft simply, the energy conservation is highly effective, is advantageous to the clean production and the environmental protection merit.

The invention discloses a preparation method of a moisture-absorbing polyester fabric. The preparation method comprises the following steps of (1) preparing an alkaline treatment solution, impregnating polyester fibers into a solution, washing and drying; (2) preparing a sucrose fatty acid ester solution, impregnating the polyester fibers into the sucrose fatty acid ester solution, washing, and drying; (3) selecting a certain mass of raw silk, and preparing into 2 2 to 5% sericin solution; (4) preparing the following solutions comprising 8 to 12wt% of epichlorohydrin solution, 1 to 5wt% of tetrabutylammonium bromide solution, 1 to 3wt% of coupling agent solution, 30 to 50wt% of JCH solution with concentration of 0.3%, and 40 to 50wt% of sericin solution; (5) impregnating the polyester fibers into the solutions in step (4), washing, and drying; (6) performing secondary impregnating on the treated polyester fibers in step (5), washing, and drying; (7) preparing the polyester fibers intothe fabric. The preparation method has the advantage that the outer surface of the polyester fiber is coated with one layer of sericin, and the sericin has good moisture absorbing property, so that the defect of poor moisture absorbing property in the polyester fiber is overcome.

The method for preparing water soluble sericin with different molecular weights by using cocoon as raw material includes the following steps: soaking cleaned cocoon crust and waste silk in water or alkalescency solution, regulating pH value of said solution to 8-12 and making high-temp. high-pressure degrading treatment, regulating temp. or pressure, treatment time and pH value of boiling solution and controlling requirement molecular weight of water soluble sericin so as to prepare water soluble sericin solution or powder. The product can be extensively used in the fields of biological material, textile finishing agent.