The invention discloses a preparation method of high-purity
phycocyanin, and is characterized in that the preparation method comprises the following steps: (1) taking a fresh spirulina
powder, fully mixing with a
phosphate buffer solution evenly, repeatedly freezing and thawing for 7-10 times to break and remove
cell walls, centrifuging to remove spirulina mud, and thus obtaining a supernatant; (2) adopting a two-step
precipitation method with a 20%-30%
ammonium sulfate and a 40%-60%
ammonium sulfate to obtain a
phycocyanin crude extract; (3) after dialyzing the crude extract, loading the sample onto a weak
anion exchange column DEAE Sepharose FF, carrying out
gradient elution after
ion exchange, collecting an outflow component with A620 / A280 of more than 3; and (4) dialyzing the collected sample, then loading the sample onto a
strong anion exchange column SOURCE30Q, carrying out
gradient elution after
ion exchange, collecting an outflow component with A620 / A280 of more than 4, again carrying out one-time
ammonium sulfate precipitation concentration, and thus obtaining the high-purity
phycocyanin having the purity of more than 4.5. The
extraction purification method is simplified in process, wide in source of the
raw material spirulina, simple in required equipment, and high in purity of the product, and has quite high application value.