499results about "Algae/lichens peptides" patented technology

Disclosed are polynucleotides and methods for expressing light activated proteins in animal cells and altering an action potential of the cells by optical stimulation. The disclosure also provides animal cells and non-human animals comprising cells expressing the light-activated proteins.

Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from Schizochytrium, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

The invention discloses a technique for extracting phycocyanin, chlorophyll and spirulina polysaccharide in spirulina, comprising the following steps: taking and weighing fresh spirulina, centrifuging and washing the spirulina, weighing spirulina clay, adding water into the supernatant, stirring evenly, and freezing and thawing twice to break the cell wall, after the second dissolving, adding water, placing for 2-8 hours, centrifuging to obtain supernatant and sediment A, extracting the sediment A by a Soxhlet extracted method with ethanol to divide the sediment A into extraction and sediment B, wherein the supernatant is used for extracting phycocyanin, the sediment A is used for extracting sodium zinc chlorophyllin and the sediment B is used for extracting spirulina polysaccharide. The extraction technique extracts the active substances such as phycocyanin, chlorophyll and spirulina polysaccharide, and fully and effectively utilizes the prior spirulina resource and avoids the resource waste.

Sustained hydrogen production is obtained by the culturing of a genetically-modified algae, where the ability of the chloroplasts to intake sulfate is reduced or eliminated compared to wild-type algae. The alga is cultured in a sealed environment in a liquid or solid medium that contains sulfur, and hydrogen is generated continuously. Alternatively, the algae may be cultured in the presence of bacteria that also produce hydrogen gas. The hydrogen produced can be collected and used as a clean energy source.

The present application provides novel regulatory elements including promoter sequences from marine microorganisms. The application further discloses DNA constructs containing these novel regulatory elements; transgenic cells, transgenic non-human organisms, and progeny containing these novel regulatory elements. Methods of modifying, producing, and using the regulatory elements are also disclosed. The regulatory elements disclosed herein are particularly suited for use in Nannochloropsis and other microalgae.

The invention provides a comprehensive utilization method for coal. The coal is transformed into methane and other clean energy chemical products and/or clean power by integrating a multi-region coal gasification sub-method, a coal-based poly-generation sub-method, a composite power source hydrogen production sub-method and/or an algae carbon absorption sub-method, and an ecological cycle mode for development and utilization of coal resources is formed, wherein in the multi-region coal gasification sub-method, methane-containing gas flow is produced in the presence of a catalyst and a gasification agent by putting coal powder into a gasification furnace comprising a partial pyrolysis region, a catalytic gasification region and a residue gasification region. The invention also provides a system for implementing the comprehensive utilization method for coal.

A production method for comprehensively utilizing oil-containing microalgae, comprising: using a high-pressure homogenizer to homogenize and break the wall of the microalgae at room temperature and high pressure. Transfer to the extraction tank, extract the microalgae oil under agitation, separate the liquid phase and the solid phase after centrifugation, wash the solid phase with water three times, add it to a spray dryer for drying to obtain microalgae powder. The liquid phase and the three times of washing liquid are added to the oil-water separator to separate into an oil phase and a water phase, and the oil phase is microalgae oil. The separated water phase is separated and purified to extract bioactive substances such as microalgae protein, microalgae polysaccharide, and microalgae liquid fertilizer. The invention has the advantages of low investment, simple operation, large-scale utilization of microalgae production method, and can produce microalgae oil, EPA, DHA, microalgae powder, microalgae protein, microalgae polysaccharide, microalgae liquid fertilizer and other high added value Semi-finished products.

Disclosed is a Volvox carteri-derived light-receiving channel rhodopsin with an improved expression efficiency on a cell membrane. Specifically disclosed is a modified Volvox carteri-derived light-receiving channel rhodopsin protein. The protein is modified to contain an N-terminal region of Chlamydomonas reinhardtii-derived channel rhodopsin-1 at the N-terminal of the Volvox carteri-derived light-receiving channel rhodopsin protein, wherein the N-terminal region is involved in cell membrane-localized expression and contains no transmembrane domain of the Chlamydomonas reinhardtii-derived channel rhodopsin-1.

An enrichment and separation method of blue green algae phycocyanin belongs to the technical field of biochemical separation engineering. The invention comprises the following steps: blue green algae disrupted solution is prepared; JDN-3 type resin enriches phycocyanin in the blue green algae disrupted solution; phycocyanin is eluted and separated; chromatographic separation is carried out on the JDN-3 type resin; freeze drying is carried out on eluent after desalination to obtain phycocyanin with certain purity; the recovery rate of albumen is up to 85.5%. The method is simple, directly uses the JDN-3 type resin to enrich the blue green algae disrupted solution without using usual ammonium sulfate salt precipitation, organic solvents, isoelectric points and other similar methods, reduces the dosage of chemical agents in the preparation process, simplifies the steps of the purification process, avoids the loss of products; the phycocyanin obtained by the chromatography of the JDN-3 type resin has higher purity which is up to A620/A280>2.0. The raw material uses fresh algae or dried algae powder, or even field blue-green algae in Taihu Lake, thereby providing a technical method for solving scale use of algal resource.

Virion-constrained nanoparticles comprising a shell of virion coat protein(s) surrounding an organic, inorganic and/or organo-metallic non-viral nanoparticle and methods of making and using.

The invention discloses a method for preparing phycocyanin by using bloom cyanophyte. Said invention uses the fresh bloom cyanophyte as raw material, and adopts the following steps: first step, extracting crude filtrate; dehydrating fresh bloom cyanophyte, adding buffer solutio, mixing them uniformly, frezing, takingout and thawing at room temperature, freezing and thawing for 3-5 times and filtering by using plate and frame; second step, ultrafiltering and purification; using hollow fibre ultrafiltration device whose trapping molecular weight is 1000-200000 daltons to remove various substances with smaller molecular weight from crude extract passed through microfiltration by using ultrafiltering membrane; third step, settling-clearing purification; removing most of small molecules from phycocyanin portion purified by means of ultrafiltration, at the same time making concentration and storing the concentrated phycocyanin portion at a certain temperature; and fourth step; hydroxyapatite column chromatography to make purification.

The invention discloses a preparation method of food grade phycocyanin protein, comprising the following steps: breaking the wall of the spirulina power; adding weak acid salt buffer solution in the wall broken spirulina power for digestion; carrying out solid-liquid separation on the spirulina power leach liquor; extracting supernatant; filtering the supernatant to obtain the crude extract; and purifying the crude extract to obtain the concentrated solution; and spraying and drying the concentrated solution. The invention also discloses a food grade phycocyanin protein prepared by the method, wherein the purity (OD620/OD280) is larger than 1.0; the content of the phycocyanin protein is larger than 50%; the content of allophycocyanin is larger than 10%; the content of phycoerythrin is larger than 2%; and the content of other proteins is smaller than 2%. The method has low production cost, is simple and effective, and is suitable for preparing mass food grade phycocyanin proteins.