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378results about How to "Easy pretreatment" patented technology

Method for determining multiresidue of veterinary drugs in animal-derived foods

The invention belongs to the technical field of food detection methods, and particularly relates to a method for determining multiresidue of veterinary drugs in animal-derived foods. The method comprises the following steps of weighing a sample, adding an acetic acid-acetonitrile solution, carrying out vibrating extraction, centrifuging to obtain acetonitrile extracting solution, loading the extracting solution obtained by centrifuging into a centrifuge tube with a mixed filler, carrying out vibrating centrifugation, sucking purified liquid, concentrating by blowing nitrogen, diluting to constant volume and detecting by UPLC / MS / MS (ultra performance liquid chromatography-tandem mass spectrometry). The method disclosed by the invention is firstly applied in detecting animal-derived foods and 107 kinds of veterinary drugs such as sulfonamides, quinolones, beta agonists in animal products are subjected to fast qualitative and quantitative detection, the detection limit is completely capable of meeting the detection requirements and the detection efficiency is greatly promoted.
Owner:烟台杰科检测服务有限公司

Tissue equivalents

A method of forming a tissue equivalent is described. A length of polyester tube is threaded over a mandrel (H) and attached at either end by clips at A and D. A block (F) is then screwed into place. The polyester is then pre-soaked by injecting through Tap (2) an acidified collagen solution for approximately one hour. After a suitable period of time the excess solution is aspirated off. Following this stage, a second, alkaline solution is injected into the apparatus which contains smooth muscle cells (SMC). Thus, neutralisation of the collagen impregnated within the fabric of the tube occurs leading to spontaneous fibrillogenesis within the interstices of the cloth, eliminating the risk of delamination. The apparatus is then incubated. The collagen contracts down onto the fabric tube and the cell-impregnated gel becomes incorporated into the presoaked collagen. The pre-impregnated collagen and the collagen provided in the aqueous mixture contract down as one into a coherent whole with the SMC. The tissue equivalent is then lined with endothelial cells and the apparatus again incubated and fed at increasing intraluminal pressures applied either statically or dynamically. This is believed to cause the vessel to become preconditioned to the pressure it will work under as an implant. This allows organization of the basement membrane which in turn promotes EC attachment and theoretically prevents smooth muscle cell hyperplasia.
Owner:TISSUEMED LTD

Systems and methods for breathing exercise regimens to promote ischemic preconditioning

Systems and methods for promoting ischemic preconditioning in individuals are provided. Ischemic preconditioning is provided by exercise treatments. The exercise treatments consist of breathing exercise regimens with breathing sequences of oxygenating and non-oxygenating phases co-ordinated with stress-relaxation cycles. The breathing sequences are designed to induce periods of ischemia. The individualized exercise treatments can induce optimal number of periods of ischemia separated by suitable intervals to provide effective ischemic preconditioning.
Owner:LIFEWAVES INT

Method for detecting tobacco-specific nitrosamines

The invention relates to a method for measuring tobacco-specific nitrosamines (TSNAs for short) by using a super efficient liquid chromatogram-tandem mass spectrum combined method, and belongs to a method for detecting TSNAs. The method comprises the following steps of: adding internal standard-containing aqueous solution of ammonium acetate into a filter sheet after trapping smoke and cigarette filters smoked completely under standard conditions or tobacco shreds, performing ultrasonic extraction, transferring 1 to 2mL of extract into a small solid-phase extraction column taking N-vinyl pyrrolidone-benzene sulfonic acid copolymer as filler, flushing the small column by using aqueous solution of methanoic acid and methanol, finally eluting the small column by using solution of aqueous ammonia and methanol solution, analyzing the eluant by using an ultra performance liquid chromatography-mass spectrometer / mass spectrometer (UPLC-MS / MS), and quantifying according to the area ratio of a component peak to an isotope internal standard peak. The detecting method can be used for detecting the TSNAs in cured and hybrid cigarette mainstream smoke, cigarette side-stream smoke, filter tip intercepting smoke and tobacco shreds, and has the advantages of simple pretreatment, accurate quantification, high instrument analysis speed, low detection limit, high sample treatment flux and the like.
Owner:HONGTA TOBACCO GRP

Pointer instrument positioning and identifying method based on machine vision

The invention discloses a pointer instrument positioning and identifying method based on machine vision. The position of an instrument panel in an instrument cabinet image is accurately positioned andan instrument reading is automatically recognized. The method comprises the steps of (1) acquiring the instrument cabinet image; (2) carrying out segmentation processing on the instrument cabinet image to obtain an instrument panel image; (3) preprocessing the instrument panel image; (4) acquiring all edge contours in the instrument panel image by using a Canny edge detection method; (5) findingall linear contours in the obtained edge contours by using Hough transform; (6) screening out the linear contours where a pointer in the instrument panel is located through length characteristics of the linear contours; (7) calculating an average value theta of an inclination angle between the two linear contours where the pointer is located; and (8) calculating out a pointer reading according toa linear relation between the linear inclination angle and an instrument scale. By the adoption of a positioning and detecting technology, the method has the characteristics of accurate positioning and reading, good anti-noise performance and efficient and rapid image processing.
Owner:HOHAI UNIV CHANGZHOU

Integrated pesticide residue detection pretreatment device and pretreatment method

The invention provides an integrated pesticide residue detection pretreatment device and a pretreatment method. The device comprises a cup body, a filter, a liquid divider, a jigger, a solid phase extraction column and a test tube, wherein a crushing homogenizer is arranged in the cup body, and the bottom of the cup body is connected with the filter; the liquid divider is arranged on the bottom of the filter to be used for carrying out standing stratification on filter liquid flowing out of the filter, and the bottom of the liquid divider is connected with a three-way valve; the jigger is connected with one opening of the three-way valve to be used for quantitatively collecting a target compound, and the bottom end of the jigger is provided with a second graduating valve; the solid phase extraction column is connected on the bottom of the jigger to be used for eluting the target compound; and the test tube is used for collecting eluent and is also connected with a nitrogen flow system used for concentrating the eluent. The device and the method integrate the processes of crushing, homogenizing, ultrasonic vibrating, suction filtration, liquid-liquid extraction, solid phase extraction, concentrating constant volume and the like; the treatment method is simple and has high efficiency, and the treatment device is convenient and simple, practical and portable and can be used for on-site operation.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES

Device for delivering medicament

The present invention relates to a device for delivery of medicament comprising a main housing; a container arranged in said housing and arranged to be connected to a medicament delivery member, wherein said container includes at least two chambers and at least one longitudinally movable plunger and wherein at least one chamber contains a medicament agent; a pre-treating arrangement acting on said container; drive force means acting on a longitudinally movable plunger rod capable of exerting a force on said container; and activating delivery means acting a said drive force means for delivering medicament via said medicament delivery member characterised in that said pre-treating arrangement comprises clickable means for manually and delay intermittently mixing said medicament agent and for manually priming said container
Owner:SHL MEDICAL AG

Method for detecting fresh degree of chicken meat by electronic nose

The invention discloses a method for using an electronic nose to detect the freshness of chicken. The invention aims at providing a method which uses the electronic nose to detect the freshness of chicken, has the advantages of rapidity and simpleness, easy use and objective and reliable detection result, and can reduce the impact of storing conditions to the detection result. The method comprises the following steps: a chicken sample to be detected is put into a sealed container and then heated in water bath with thermostatic control; the heated chicken sample is put into a test room, and then smell sent by the sample is detected by a gas sensor array which consists of four gas sensors of different types; the data which is aquired by the gas sensor array is collected and processed; and then the freshness of chicken is judged according to the processing result of the data. The detection method uses an electronic nose which consists of four gas sensors of different types to detect the chicken sample, and has the advantages of objective, accurate, and fast detection result, good repeatability, simple analysis process and small using amount of sensors, and reduces the extraction of eigenvalues.
Owner:TIANJIN UNIV OF COMMERCE

Method for fast analyzing pesticide residue in fruits and vegetables

The invention discloses a method for fast analyzing pesticide residue in fruits and vegetables, which is implemented by weighing a sample, adding anhydrous sodium acetate and anhydrous magnesium sulfate, adding acetic acid acetonitrile solution, centrifugating after shaking and extracting, putting the centrifugated acetonitrile extract into a centrifugating tube filled with mixed filler, shaking, centrifugating, sucking the cleaning fluid, concentrating and evaporating to be dry, dissolving the mixed solution with the volume ratio of acetonitrile and water being 1:1, and performing UPLC / MS / MS detection. The invention uses fruits and vegetables as matrix, uses the matrix dispersion solid extraction technology to pre-treat the sample to realize fast detection on 130 types of pesticide residue such as avermectins, aldicarb, Cyromazine and the like, having the characteristics of fast and exact detection result, high efficiency and low price.
Owner:烟台杰科检测服务有限公司

High Recovery Drinking Water Process

Disclosed is a process that uses reverse osmosis (RO) or nanofiltration (NF) membranes and ion exchange (IX) water softening resins to maximize the production of drinking water, purified industrial service water or recycled purified municipal or industrial effluents. The membrane and ion exchange units are configured in a manner that enables operation of the system at high permeate recoveries in the range 90% to 99%, thus producing purified drinking water with a minimum volume of wastewater to drains and a very low membrane cleaning or membrane replacement frequency. The process mitigates membrane fouling and scale formation and minimizes the use of water treatment chemicals including acidic and alkali solutions and sodium chloride salt that is normally required to regenerate the IX resin.
Owner:AL SAMADI RIAD A

Micro fluidic biological chip based on micro balls

It is a micro flow control biological chip based on micro ball, which is used to measure protein and nucleic acid large molecule and can be widely used in the fields of clinic measurement, quarantine inspection, environment monitoring, medicine filter, microbiological assay. The microbiological chip comprises the upper cover 1 and bottom ground 2, wherein, the cover 1 is located with step micro path and the micro path is located with micro balls as probe molecule of different sizes and the both ends of the micro path are located with one entrance 16 and an exit 17. The step micro path has at least two steps and two kinds of micro balls, wherein, one step responds to one micro ball and the size of the micro ball serves as different probe molecules fixed on the micro balls.
Owner:SOUTHEAST UNIV

Interactive video advertisement placing method and system

The invention provides an interactive video advertisement placing method and system. The method comprises the steps of obtaining and caching a current video image; identifying a background and a shape in the cached video image; separating the background, searching advertisement according to the shape of the background and returning a search result; and replacing the current background and transmitting the video image to a live broadcast platform for distribution. According to the method and the device, an anchor, the background and the advertisement are linked; the advertisement is searched according to the shape of the background; the precise delivery of the advertisement is greatly improved; the interaction between the advertisement and the anchor is enhanced; a preprocessing process is increased before the advertisement is placed; the preprocessed advertisement and the image frame match well and are integrated well; and the visual and sense experience of a user are improved.
Owner:SHANGHAI ZHANGMEN TECH

Method for detecting residues of 202 pesticides in tobacco leaves

The invention discloses a method for detecting the residues of 202 pesticides in tobacco leaves, comprising the following steps of: extracting a tobacco powder sample by an organic solution; purifying a QuEChERS reagent bag; centrifuging; and then utilizing a gas-phase triple quadrupole mass spectrum provided with a procedure temperature-rising sample feeding opening to detect the residues of the pesticides in the tobacco leaves. The method disclosed by the invention has the advantages of less interference, wide covering range of pesticide varieties, simplicity and convenience for pre-treatment, solvent conservation, accuracy and flexibility and the like.
Owner:中国烟草总公司贵州省公司

Face detection method and system based on image on-line learning

The invention discloses a face detection method and system based on image on-line learning. The face detection method based on image on-line learning comprises the steps of (1) preprocessing, wherein illumination compensation and graying processing are carried out on an image to be detected, image enhancement is carried out, nonlinear smooth filtering is carried out, denoising is carried out on the image, gray levels of pixels are normalized to obtain a high-quality gray level image, and then size normalization processing and edge detection processing are carried out; (2) carrying out face gesture detection, wherein the positions of the human eyes are determined, a human face area is divided, the rotation angle of a human face in the pitching dimension, the rotation angle of the human face in the depth dimension and the rotation angle of the human face in the plane dimension are detected, and whether the human face has an expression or not is automatically judged; (3) carrying out face detection, wherein the position of the human face in the image is determined, organs of the human face are located, and gray features of the image are selected, and are transmitted to a detection template which is trained in an off-line mode to carry out judgment; (4) updating, wherein the image which is processed through detection serves as a new sample to be applied to learning of a multi-layer cascade AdaBoost classifier, and the weight of set characteristic values of the multi-layer cascade AdaBoost classifier is updated. According to the face detection method and system based on image on-line learning, the accuracy rate of face detection can be improved.
Owner:SHANGHAI JUNYU DIGITAL TECH

Method for preparing Carthaginian apple raw juice

InactiveCN101273790AImprove qualityAccess to avoid and minimizeFood preparationPectinaseNutritive values
The invention relates to a preparing method for pomegranate juice; the method comprises the following steps that a fresh pomegranate is soaked by disinfecting solution, soaked and washed by ultrasonic wave and clear water and then washed by clear water; grains of the pomegranate are peeled after being cut, and the pomegranate juice is squeezed out by beating; a method adopting the combination of vacuum degassing and antioxidant is used for degassing; pectin of the pomegranate juice is hydrolyzed by adding pectinase; diatomaceous earth is used for removing suspended matters; colloidal particles, microorganisms and enzymes are removed by ultrafiltration; the ingredients are regulated; a method of riadiationsterilization is used for sterilizing, and then sterile filling and covering are carried out, thus preparing the pomegranate juice. The highest temperature of processing is less than 50 DEG C in the whole process of production of the pomegranate juice, thus keeping the color, flavor, nutritive value and functional ingredients of fresh pomegranate extracted juice furthest, leading the products to have longer guarantee period and realizing industrial production at the same time.
Owner:吐地·艾力

Ultra-large type plate shearing machine plate shearing edge curve detection system and method

The invention discloses an ultra-large type plate shearing machine plate shearing edge curve detection system and method. The plate shearing edge curve detection system mainly comprises an optical imaging unit, an image acquisition and processing unit and a main control computer. The plate shearing edge curve detection method includes the following steps: first, CCD sensors and lenses which are installed on an ultra-large plate shearing machine are utilized to perform real-time image acquisition on a plate shearing edge under cooperation of strip-shaped light sources; after preprocessing of collected images, the images collected by adjacent industrial cameras are spliced, an image edge is detected after edge burrs are removed, and finally a coordinate axis and coordinate points are selected and a fitting technology is utilized to detect an actual machining curve of a sheared plate. The ultra-large type plate shearing machine plate shearing edge curve detection method utilizes image real-time acquisition and image processing technologies to detect the plate shearing edge curve, and the detection method can achieve higher detection precision and detection efficiency compared with a traditional detection method.
Owner:JIANGSU UNIV

Two-dimensional highly effective liquid phase chromatographic system and its uses

The present invention relates to high-performance liquid chromatography, specifically a two-dimensional high-performance liquid chromatography system and its application, consisting of five high-pressure liquid chromatography solvent delivery pumps, a solvent mixer, a ten-way valve, and a six-way valve 1. Two liquid chromatography analysis columns suitable for the separation of hydrophobic components and hydrophilic components and a component transfer column. The system of the present invention is suitable for the separation and detection of a variety of different hydrophobic small molecular components in complex system samples. It is easy to operate, good in flexibility, strong in universality, short in time consumption, and can be automatically separated on two chromatographic columns at the same time. The hydrophilic and hydrophobic components in complex system samples can significantly improve the resolution and peak capacity of the analysis on the basis of meeting the qualitative and quantitative analysis requirements of the sample components.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

Online plant species identification method based on geometric invariant shape features

The invention relates to an online plant species identification method based on geometric invariant shape features. The method includes 1, allowing a browser to acquire leaf image and transmit to a server after pre-processing; 2, allowing the server to calculate the leaf shape features according to pre-processed leaf images, establishing feature vectors, storing a trained moving center hypersphere classifier in the server, allowing the moving center hypersphere classifier to perform species identification according to the feature vectors, outputting the plant species code, querying a leaf information database according to the plant species code, and feeding the final identification result back to the browser; 3, allowing the browser to display the final identification result including the leaf, flower, fruit and seed images of the identified species and the scientific instruction information of the species. Compared with the prior art, the method has the advantages of fine real-time performance, convenience and high accuracy.
Owner:TONGJI UNIV

Biological engineering prepared health care milk as well as matched method and product

A biologically engineered health-care milk features that the milk cow is used as a biologic engineering processor to make the active components of 18 Chinese-medicinal materials including ganoderma, ginseng, champignon, aloe, etc come into fresh milk, or said active components are used as the additive of milk food. The disease resistance and milk output of milk cow can also be increased.
Owner:吴跃飞

Single-component modified high chlorinated polyethylene weathering resistant residual rust painting

The invention relates to the technical field of forming painting materials using an organic film as a main body, in particular to a weathering resistant single-component high chlorinated polyethylene anticorrosion painting applied to the surface of a residual rust steel structure, and the residual rust painting consists of a film-forming resin matrix which is the compound resin-modified high-chlorinated polyethylene with the molecular weight ranging from 15,000 to 25,000, residual rust painting dyes, an antirusting agent, a dispersing agent, an anti-settling agent and a defoaming agent; and the residual rust painting can be used in the steel structure with a less than 80 mu m rusty layer and has excellent weathering resistant and ageing properties, low cost and easy construction, and is suitable to be used for the heavy corrosion protection projects of the steel structures of ships, trains, bridges and ocean petrochemical industry.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI

Gas density intelligent test system and method

The invention relates to a gas density intelligent test system and a method thereof. The system mainly comprises a light interference gas sensor, a signal conditioning circuit, a temperature sensor, a pressure sensor, an A / D converter, a data collecting and processing module, a timing clock signal generator and an upper computer. The invention is characterized in that the light interference gas sensor, the temperature sensor and the pressure sensor measure gas density, temperature and pressure respectively; the upper computer reads the data of gas density, temperature and pressure in the data collecting and processing module and the data of year, month, date and time generated by the timing clock signal generator, and the true gas density value of the measured point is calculated by the upper computer and displayed on a display, or the variation curve of the true gas density value along with sharp time is displayed. The invention has simple operation and high measurement accuracy, strengthens real-time data processing and revising capabilities, and proposing a new approach for automatically detecting and telemetering coal mine underground gas.
Owner:ZHONGBEI UNIV

Preparation method of high-performance titanium/steel bimetallic composite plate

The invention discloses a preparation method of a high-performance titanium / steel bimetallic composite plate, and belongs to the technical field of preparation of bimetallic composite plates. A pure titanium slab and a low-carbon steel slab are subjected to annealing treatment, the hardness of the slabs is reduced, and the hardness of the pure titanium slab and the hardness of the low-carbon steelslab are close; then the to-be-composited surfaces of the slabs are cleaned, degreased and ground, wherein the grinding stripe direction is perpendicular to the rolling direction; then the pure titanium slab and the low-carbon steel slab are stacked to obtain a combined slab, the head of the combined slab is riveted, the combined slab is subjected to single-pass cold rolling pre-compounding, andthen heating is carried out; and finally, single-pass hot rolling final compounding is carried out, and the high-performance titanium / steel bimetallic composite plate is obtained. The method is low inequipment requirement, short in process flow, high in production efficiency and low in production cost. The interface bonding strength of the prepared titanium / steel bimetallic composite plate is larger than 230 MPa, the area bonding rate is 100%, and the method is particularly suitable for preparation of high-performance thin titanium / steel bimetallic composite plates or thin-titanium-layer titanium / steel bimetallic composite plates.
Owner:UNIV OF SCI & TECH BEIJING +1

Soil cadmium passivator as well as preparation method and application thereof

The invention provides a soil cadmium passivator. The soil cadmium passivator is prepared from biological carbon, quick lime and sodium alginate; by means of the quick lime in the soil cadmium passivator, a pH value of soil can be improved, available cadmium in an ionic state is converted into a cadmium sediment in an invalid state such as cadmium hydroxide; the sodium alginate has a good chelating effect on heavy metal cadmium; the biological carbon has certain adsorbing effects on the available cadmium in the ionic state, the cadmium hydroxide and invalid cadmium such as in cadmium a complexing state; the biological carbon, the quick lime and the sodium alginate in the soil cadmium passivator can be mutually matched and cooperated at a specific mixture ratio, so that the effects of reducing the content of the cadmium in an available state in soil and the content of the cadmium in a living body grown in the soil are realized; the soil cadmium passivator also has the advantages that a PH value in the soil is improved, the functions of the soil are improved, and secondary pollution is avoided.
Owner:INST OF SOIL FERTILIZER & RESOURCE ENVIRONMENT JIANGXI ACAD OF AGRI SCI

Three-dimensional mesh model tetrahedralization method

The invention discloses a three-dimensional mesh model tetrahedralization method. The method comprises the following steps that (1), the initial position of a model is preprocessed; (2) a body centered cubic structure is established; (3) node symbols and points of tangency of boundries of a tetrahedron with the node symbols at the two ends opposite are calculated; (4) the points of tangency are moved; (5) the boundaries are tetrahedralized again. According to the three-dimensional mesh model tetrahedralization method, based on a body-centered cubic mesh tetrahedralization algorithm, preprocessing of principal component analysis of the three-dimensional model is added, a movement mode of the points of tangency of the boundaries of the model is changed, the points of tangency are moved to feature points of the model, and the positions of nodes of a final tetrahedral mesh are optimized through a density energy error function. After principal component analysis is adopted in the three-dimensional model, the quality of an initial tetrahedron element is improved; movement of the points of tangency to the feature points of the model is improved, so that local features of the tetrahedralized model are guaranteed; the quality of the final tetrahedral mesh is optimized through the density energy error function.
Owner:HANGZHOU MEIDEER INTELLIGENT TECH CO LTD

Channel level hierarchical dynamic routing method of electric power automation front-end system

The present invention discloses a channel level redundancy-backup and loading-sharing front-end hierarchical dynamic routing method which not only can realize the channel level redundancy backup, but also can support the loading sharing of the front-end service node for increasing the reliability and the processing capacity. The front-end system of the electric power automation device is introduced with a hierarchical design mode. The downmost bottom layer is a device management layer of the communication medium. Then a frame layer, an agreement explaining layer and a data processing layer are provided sequentially. The device layer mainly shields the communication characteristic of various different mediums and provides the unified data reading-in and writing-out interface of the frame application. The realizing of the frame layer mainly relates to the management of the channel. The data transmission characteristics of various applications are integrated. The system supports a plurality of operating modes. Each channel can be managed singly or integrally. The agreement explaining layer completes the analysis of the various different aggrements and provides an unified data interface for the data processing layer. The front-end system of the electric automation device supports a plurality of front-end nodes and the group management. The front-end nodes and the node groups have a parallel operation mode based on the channel management and realize the data shunting and loading sharing based on the channel.
Owner:BEIJING SIFANG JIBAO AUTOMATION +1

Method for measuring released alkaloid amount in mainstream smoke of cigarette

The invention relates to a method for measuring the released alkaloid amount in the mainstream smoke of cigarette. The method comprises the following steps: (1) preparing an alkaloid standard solution; (2) preparing a solution of a sample to be tested; (3) analyzing the alkaloid standard solution by GC-MS / MS, taking the ratio of the peak areas of the alkaloid and an internal standard substance as the vertical coordinate, taking the concentration of the alkaloid mixed standard solution as the horizontal coordinate, and carrying out regression analysis to obtain a regression equation and related coefficient; (4) analyzing the solution of the sample to be tested by GC-MS / MS, and calculating the released alkaloid amount according to the regression equation. The method has the advantages that the alkaloids in the smoke of cigarette can be analyzed and detected at the same time, the pretreatment is simple, the detection result is accurate, the repeatability is good, the sensitivity is high, and the method can be used to measure alkaloid in the smoke of cigarette and other complicated matrix.
Owner:CHINA TOBACCO GUIZHOU IND

Gas turbine

InactiveUS6877953B2High flow lossStable flow characteristicsEngine manufactureWind motor controlGas turbinesAerospace engineering
A gas turbine with a compressor area has a rotor shaft 2 to carry rotor blades 1 and a casing 4 to carry stator vanes 3, with the related rotor blades 1 and stator vanes 3 forming an axial clearance space 5 between one another, wherein, in the area of the axial clearance space 5, at least one wall of the rotor shaft 2 and / or of the casing 4 is provided with an arrangement of fins 6 which are inclined against the axial direction.
Owner:ROLLS ROYCE DEUT LTD & CO KG

A kind of method for measuring paraquat blood concentration

The invention discloses a method for measuring blood concentration of paraquat. The method comprises the following steps of: (1) pretreating a sample, namely adding aqueous solution of an internal standard substance and a protein precipitation agent acetonitrile into a plasma sample, performing whirl mixing, centrifuging and sampling supernatant; (2) separating the sample, namely adopting a universal C18 liquid chromatographic column, and using an ion pairing agent in an acid mobile phase, wherein the acid mobile phase is mixed solution of 3mmol.L<-1> aqueous solution of sodium dodecyl sulfate, 0.2 percent of aqueous solution of trifluoroacetic acid, acetonitrile and water; and (3) detecting by using a diode array detector, namely detecting by using the diode array detector at a detectionwavelength of 250 to 260nm, measuring peak areas of the internal standard substance and the paraquat, and calculating the blood concentration of the paraquat through least square method linear regression. The plasma sample is easy and convenient to pretreat, the detection process is sensitive and rapid, toxic substances and the blood concentration thereof can be rapidly determined in actual application, and the method is high in clinical application value.
Owner:HENAN UNIV OF SCI & TECH
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