187 results about "Quechers" patented technology

The invention discloses an analysis method for simultaneously measuring residues of 12 sulfonamide medicaments, 19 quinolone medicaments and 8 benzimidazole medicaments and metabolites thereof in chicken liver by quick, easy, cheap, effective, rugged and safe (QuEChERS) extraction and purification-ultrahigh performance liquid chromatography-tandem mass spectrometry (QuEChERS-UPLC-MS / MS). The method comprises the following steps of: extracting a sample by using 1 percent acetic acid-acetonitrile solution, purifying the sample by using NH2 adsorbent, and degreasing the sample by using n-hexane;and then performing separation by using a Kromasil Eternity C18 chromatographic column (100mm*2.1mm, 2.5mum), performing gradient elution by using 0.1 percent formic acid and methanol as mobile phases, performing ionization in an electron spray positive ion (ESI<+>) mode, performing detection in a multi-reaction monitoring (MRM) mode, and performing quantification by an internal standard method. The 39 medicaments have good linearity (r is more than 0.98) in a blank adding concentration range of 5 to 100mug / kg, the average recovery rate of the medicaments in the adding level range of 10 to 50mug / kg, the relative standard deviation (RSD) is 1.5 to 23.4 percent, the limit of detection (LOD) of the 39 medicaments is 5mug / kg, and the low limit of quantification (LOQ) is 10mug / kg. The method is simple, convenient, quick, sensitive, accurate and rugged, and is suitable for confirmation and quantitative determination of the residues of the residues of the sulfonamide, quinolone and benzimidazole medicaments in the chicken liver.

The invention relates to a method for detecting a plurality of mycotoxins in a sesame paste, and particularly relates to a method which carries out pre-treatment by an optimized dispersive solid-phase extraction method (QuEChERS), and simultaneously detects 26 mycotoxins in sesame paste by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS / MS). The method particularly comprises: extracting a sample twice with acid-containing acetonitrile-aqueous solutions with different concentrations, adding magnesium sulfate and sodium chloride into the sample extract for salting out, performing centrifugation, adding n-hexane into the obtained supernatant, performing vortex oscillation and degreasing, purifying the product with C18 and magnesium sulfate, performing vacuum concentration, dissolving the residues again with methanol and water in order to obtain a sample analytic solution, and detecting the solution by a multi-reaction monitoring mode of ultra-high performance liquid chromatography-tandem mass spectrometry. The method improves the detection efficiency, saves detection cost, is high in sensitivity, high in repeatability, and high in adding standard recovery rate, and can be extended to detection of other samples with a high grease content.

The invention relates to a detection method for plant growth regulator residues in fruits. The detection method comprises the following steps: the QuEChERS method is adopted to extract and purify the plant growth regulator residues in fruits, so as to enable the adding standard recovery rate of the target compound to reach 71%-98%; the RSD ( relative standard deviation) is not higher than 6%; the sensitivity and selectivity are favorable and the recovery rate is stable; the liquid chromatograph tandem mass spectrogram detection method is adopted to simultaneously detect forchlorfenuron, ethephon, 4-sodium chlophenoxycetate and 2,4- sodium acetate-dichlorobenzene oxygen; the detection limits range from 0.5 microgram / kg to 100 microgram / kg respectively; the sensitivity requirement of national limited standard is met. The detection method has favorable sensitivity and selectivity and can serve as a method for detecting the plant growth regulator residues in fruits.

The invention provides a sample preprocessing purifying adsorbent used for detecting the pesticide residues of fruits and vegetables. The purifying adsorbent comprises, by weight, 0-50 parts of C18, 0-50 parts of graphitized carbon black and 10-50 parts of Fe3O4@SiO2@PSA. The purifying adsorbent can be used to preprocess a sample in order to combine extracting and purifying steps of preprocessing methods in the prior art into one steps, so the operating time is effectively saved, and a centrifuge step between the extracting step and the purifying step is omitted; and compared with existing wide-applied rapid preprocessing methods QuECHERS, a preprocessing method in the invention reduces the consumption time by 61.1%, and allows 50 samples to be simultaneously preprocessed in 70 min only.

The invention discloses a determination method of residual amount of glufosinate, glyphosate and aminomethyl phosphoric acid in food. The determination method of residual amount of glufosinate, glyphosate and aminomethyl phosphoric acid in food comprises the following steps: in the sample pretreatment, firstly, extracting glufosinate, glyphosate and aminomethyl phosphoric acid in a food sample by a methanol water solution in sample pretreatment; then purifying an extracting solution by selecting a C18 dispersive solid-phase extraction agent; and finally, filtering, deriving, and determining three compounds qualitatively and quantitatively synchronously by combining with high performance liquid chromatography-tandem mass spectrum. According to the method provided by the invention, the operation is simple and convenient, and the reproducibility is good, and impurities in a sample extraction solution can be removed effectively; the pretreatment is the supplement and improvement of the existing QuEChERS method; the recycling rate can reach 70.5%-91.2%; the relative standard deviation is 4.5%-7.83%; the limit of quantification is 50micro-g / kg; the determination method can completely meet the technical requirement on safety detection of China, European Union, America and Japan, and can provide powerful technical support for food safety and export trade healthy development for people of China.

The invention provides a method for detecting various phthalic acid ester plasticizers at the same time. The method includes the steps that firstly, a sample is extracted and purified in an optimized QuEChERS pretreatment mode; secondly, analysis is carried out in a gas chromatography-tandem mass spectrometry mode; finally, accurate qualitative and quantitative analysis is carried out with an isotope internal standard method. The method can be applied to detecting twenty-three phthalic acid ester plasticizers in plant source character foods such as common Chinese cabbages, tomatoes, strawberries, grapes, shiitake mushrooms and agaric at the same time. By means of the method, the plasticizers introduced through external source ways are avoided to the maximum degree, the pretreatment step is simple, consumed time of solid-phase extraction and purification is shortened, and consumed solvents and argent compound losses of the solid-phase extraction and purification are reduced; the method has the advantages of being sensitive, accurate, good in stability, short in consumed time and easy to popularize; the method can be suitable for detecting mass samples at the same time.

The invention relates to a full-automatic QuEChERS preprocessing all-in-one machine and a preprocessing method. The preprocessing all-in-one machine comprises a reagent container, wherein the reagent container is connected with a container distributor; the container distributor extends out to form liquid discharge pipes; the liquid discharge pipes are connected with a plurality of extracting centrifugal pipes; each extracting centrifugal pipe is connected with an impurity-removing centrifugal pipe through a first guide pipe; the preprocessing all-in-one machine further comprises pulverizers and an inner sucking pump; the pulverizers pulverize samples in the extracting centrifugal pipes; the inner sucking pump pumps a supernatant liquor in the centrifugal pipes into the impurity-removing centrifugal pipes; the impurity removing centrifugal pipes are connected with filters through second guide pipes; the supernatant liquor in the impurity removing centrifugal pipes is pressurized by a pressure pump and enters sample introduction containers through the filters. The preprocessing all-in-one machine combines the process of sample pulverization, liquid adding, extraction, centrifugal separation and solid phase extraction into one, saves time, simplifies operation and steps, reduces errors, and is efficient and high in safety.

The invention discloses a full-automatic QuECHERS experimental device and an experimental method. The full-automatic QuECHERS experimental device comprises a multifunctional mechanical arm, a cap screwing module, a liquid adding module, a centrifugal module, a salt adding module, a ceramic proton homogenization module, a nitrogen blowing concentration module, a vortex uniform mixing module and anoscillation module, and can automatically complete sample transferring, addition of extraction liquid, extraction salt and ceramic homogenization protons, oscillation uniform mixing, centrifugal separation and vortex uniform mixing actions; A method adopting the full-automatic QuECHERS experimental device to carry out experiments mainly integrates the processes of sample liquid adding extraction,centrifugal separation, solid phase extraction and nitrogen blowing concentration, so that the time is saved, the manual operation is reduced, the automation is improved, and the errors are reduced.

The invention discloses a detection method for quickly screening multiple veterinary drug residues in fish flesh. The method comprises the following steps: preparing 31 kinds of standard solutions, determining the standard solutions, drawing a standard working curve, preprocessing a sample by adopting a QuEChERS technology to effective reduce the interference influences of matrixes on target compounds (including beta-lactam, non-steroidal anti-inflammatory drugs and other 29 kinds of veterinary drug residues), and determining the processed sample by a ultra-high performance liquid chromatograph-high resolution mass spectrometer. The method for determining the 31 kinds of veterinary drug residues in fish flesh has high sensitivity, and the detection limit is 0.1-2.5 [mu]g / kg; and the detection method also has the advantages of simplicity, rapidity, good accuracy, high sensitivity and good precision, is suitable for large-scale detection of fish flesh samples, can accurately quantitatively and qualitatively analyze the target compounds, and avoids the false positive phenomenon.

The invention discloses a QuEchERS method package for efficiently and homogeneously extracting residues of a pesticide and a veterinary drug. The QuEchERS method package comprises an extraction pipe containing ammonium sulfate, a buffer salting-out phase-splitting salt package containing a mixture of anhydrous magnesium sulfate and sodium chloride, a needle cylinder type membrane filter containing a mixture of PSA, graphitized carbon black and a C18 reverse phase silica gel filler, and a salting package tool, wherein the PSA is an N-propylethylendiamine-modified silica gel filler and the C18 reverse phase silica gel filler is an octadecyl-modified reverse phase silica gel filler. According to the method, the technical feature of the combination of extraction, salting-out phase-splitting and dehydration purification is fully utilized, devices, materials and operating steps are reasonable to form, the structure is simple, and the operation is easy and convenient.

The invention discloses a solid-phase adsorption material and an application thereof to a QuEChERS method. The solid-phase adsorption material disclosed by the invention adopts a carbon material, a modified silica gel material and a magnetic ferroferric oxide composite material as adsorption materials; and the price is low and the commercialization is easy to realize. The QuEChERS method comprises the following steps of: firstly extracting sample homogenate added with salt by using acetonitrile; then adding anhydrous magnesium sulfate to remove water and using the magnetic adsorption material to remove a large amount of interference objects in the acetonitrile; and finally, directly carrying out detection and analysis on a treated solution. The magnetic adsorption material has a magnetic separation performance and can be rapidly separated from the solution under the effect of an external magnetic field; and the operation is simple and the analyzing time is effectively reduced.

The invention discloses a method for screening pesticide and veterinary drugs in infant formula food by ultra-high performance liquid chromatography-quadrupole static electric field track ion trap mass spectrum, and belongs to the technical field of food safety inspection. The method comprises the following steps: preprocessing a sample by an optimized QuEChERS method; screening by applying ultra-high performance liquid chromatography-quadrupole static electric field track ion trap mass spectrum; selecting a positive ion or negative ion mode for scanning by adopting full-scan / data dependence acquisition, full-scan / variable data independence acquisition and full-scanning / full-ionic acquisition fragmentation acquisition scan modes to obtain complete primary and secondary maps; and processing the primary and secondary maps by applying Exact Finder 2.5 software to extract compound information. According to the method, information in the established spectrum library is compared with the screening result of a to-be inspected object so as to ensure the composition of pesticide and veterinary drugs in an infant formula food. The mass spectrum instrument used in the method has the main advantages of high resolution ratio, accurate qualitative and quantitative results, high sensitivity, high quality precision and the like. The method can be applied to analysis of complex substrates.

The invention provides a method for determining 16 mycotoxins in tea leaves by ultra-high performance liquid chromatography-tandem mass spectrometry, and belongs to the technical field of analytical chemistry. The method comprises: extracting a sample by formic acid / acetonitrile (10: 90); adding a QuEChERS salt packet, shaking and centrifuging; treating an extracting solution through an OASIS PRIME HLB small column and a Dspe purification pipe, separating by using a Waters HSS T3 chromatographic column, adopting a multi-reaction ion monitoring (MRM) mode of simultaneously scanning positive ions and negative ions, matching a tea substrate with a standard solution, and quantifying by using an isotope internal standard method. The method is stable, accurate, sensitive and rapid, and can meetthe requirements of analyzing various toxin residues in various tea leaves.

The invention relates to a method applicable to simultaneous and rapid determination of organic phosphate fire retardants (OPFRs) in different food sample matrixes. The method is characterized in that residues of the OPFRs in the foods are extracted and purified by an improved QuEChERS method, the matrix interference of a target compound is effectively eliminated, and the matrix effect is 92.3%-116.1% (except triphenylmethyl phosphate is 65.9%). the method has the advantages that the selectivity and the stability are good, the average recovery rate is 78.6%-127.3%, and the relative standard deviation is 3.1%-9.7%; an ultra-high performance liquid chromatography-tandem quadrupole mass spectrometer (UPLC-MS / MS) is adopted for qualitative and quantitative detection, the sensitivity is good and the quantification limit is 0.05-0.42ng.g-1; and synchronous and rapid detection of multiple residues of trace OPFRs in complex matrixes of different food sample types is realized and the technical defects in the current field are made up.

The invention relates to the technology of inspection and quarantine, in particular to a method for simultaneously detecting various preservatives remaining in orange with a liquid chromatograph / mass spectrometer. The method is to adopt the QuEchERS purification technique, the ultra-high performance liquid chromatography and the electrospray tandem quadrupole mass spectrometry to detect the residuals, optimize analytical procedures and parameters, and study detection of the plurality of preservatives for retaining freshness of the orange with the qualitative method and the quantitative method, wherein the preservatives comprise carbendazim, thiabendazole, prochloraz, imazalil, thiophanate methyl, prochloraz and 2,4-D (2,4-dichlorophenoxyacetic Acid). Therefore, the detection technology which is high in sensitivity and accuracy, quick in detection and capable of simultaneously detecting the preservative residuals can be established.

The invention relates to the analytical chemistry and food safety field and in particular relates to a method for detecting 205 kinds of pesticide residues in rice. According to the method, a sample is extracted by acetonitrile, extraction solution is purified by a QuEChERS method, a chromatographic column shown as below is adopted as an analytical column, methyl alcohol-ammonium formate aqueous solution is used as a moving phase, then gradient elution, electrospraying and positive ion scanning are carried out to separate and detect 205 kinds of pesticide residues; mass spectrum conditions are optimized and the optimal mass spectrum analysis condition is established while the sensitivity of all ingredients is considered; the detection limits of 205 kinds of pesticides are all lower than 10mu g / kg, and the range of the recovery rate of 205 kinds of pesticides is within 50-130%. According to the method, the detection limit, the recovery rate, the precision and other technical indexes all meet the domestic and foreign requirements for detection of pesticide residues in rice.

The invention relates to a QuEChERS extraction method for poisonous substance extraction, which is especially suitable for extracting unknown poisonous substances in food poisoning samples. The scheme is that the extraction method comprises the following steps: at first, extracting homogenized or mixed samples with acidiferous acetonitrile or Na2 EDTA to obtain extract liquor; adding NaC1 and Na2SO4 in the extract liquor, and mixing to obtain mixed liquor; carrying out supersonic centrifuge on the mixed liquor to obtain supernate, enabling the supernate to be subjected to C18 and anhydrous sodium sulfate purification to obtain an extracting solution, and then detecting and analyzing the extracting solution. The extraction method and application are suitable for various different matrixes: pork, pork liver, milk, eggs, fruits, vegetables, gastric juice, blood and urine, are high in recovery rate and quick and reliable to test, are used for quickly screening the actual poisoning samples, and facilitate the timely treatment and criminal investigation on poisoned personnel.

The invention discloses a pretreatment method for strong-polarity pesticide residue detection. The pretreatment method comprises the steps of extracting strong-polarity pesticides from a sample through a methanol aqueous solution, purifying an extracting solution through a dispersive solid-phase extraction agent, and filtering the extracting solution for instrument analysis. The pretreatment method disclosed by the invention is a complementation and an improvement on the existing QuEChERS method, so that the shortcoming that the conventional internationally accepted QuEChERS method cannot be used for detecting the strong-polarity pesticides is overcome. The pretreatment method is easy to operate and high in reproducibility; the recycling rate of a strong-polarity compound is effectively guaranteed; meanwhile, impurities in the sample extracting solution are effectively removed during purification, so that pollution to a detection instrument is relieved, and the detection sensitivity is greatly improved.

The invention discloses a UPLC-MS / MS method for measuring antiviral drug residuals in chicken. The method comprises the steps that a QuEChERS method is used for sample pretreatment and sample detection analysis, the sample pretreatment comprises sample extraction and purification, the sample extraction is extraction of a combination state sample in a hydrochloric acid-methanol mixture after enzymolysis under the acidic condition, and the sample purification is dispersive solid-phase extraction purification on an extracting solution by using C18 powder and PSA powder adsorbing agents after anhydrous sodium sulfate dehydration. Four types of 12 kinds of antiviral drug residuals with large compound property difference can be measured simultaneously and rapidly by improving the sample extraction and purification method and the chromatography-mass spectrometry conditions, the operation steps are simplified, the detection time is saved, the detection efficiency is improved, and the detection cost and the usage amount of the organic solvent are reduced.

The invention relates to an analysis method for rapidly screening pesticide and metabolite residue in tea. The analysis method comprises the steps of analyzing by Sin-QuEChER dispersive solid-phase extraction combined with an ultrahigh performance liquid chromatography-quadrupole-static field rail well high-resolution mass spectrum method; extracting a sample with 0.1% formic acid acetonitrile solution; performing salt precipitation oscillation and centrifugation; further purifying with Sin-QuEChERS Nano dispersive solid-phase extraction column; performing separation with Thermo Accucore aQ (2.1 millimeter by 150 millimeter and 2.6 micrometers) chromatographic column, and performing gradient elution with methyl alcohol, 0.1% methyl alcohol and 5mmol / L ammonium formate solution; and analyzing in a Full MS / ddMS2 scanning mode under a positive ion mode. The method is simple, convenient, rapid, efficient and accurate in handling process and can be used for rapidly screening multiple pesticide and metabolins in the tea.

The invention discloses an animal source food residual medicine detection method, which comprises the following steps of performing pretreatment on a sample by a QuEChERS method; performing gas chromatgraphy-mass spectrometry combined use detection, wherein according to the method of performing pretreatment on the sample by the QuEChERS method, a test sample is weighed and is put into a centrifugal pipe; ethyl acetate is added; vortex uniform mixing and centrifugation are performed; an organic layer is transferred into another centrifugal pipe; then, a ethyl acetate and ceramic homogeneous proton is added; manual oscillation and centrifugation are performed; supernates in the two times are merged; constant-temperature nitrogen blowing concentration is performed until the materials are nearly dry; acetonitrile is added for re-dissolution; vortex uniform mixing is performed; a QuEChERS purifying agent is added; vortex uniform mixing is performed; the materials pass through a filtering film and wait for machine entering testing. The method has the characteristics that the use operation is simple; the time consumption is short; the sensitivity is high; the repeatability is good. The method is suitable for large-scale application.

The invention discloses a method for simultaneously detecting seven fungal toxins in original auxiliary materials of baijiu. According to the method, an improved QuEChERS method is adopted for extraction, further purification is not needed, high performance liquid chromatography is utilized for achieving separation of the fungal toxins, combined quadrupole-electrostatic field orbitrap mass spectrometry (Q-Orbitrab) is achieved, efficient parent ion selectivity and high-resolution accurate molecular weight (The mass number can be accurate to five digits after the decimal point.) are combined, accurate qualitativeness and quantification of the fungal toxins in baijiu and the original auxiliary materials can be achieved, and quantification is conducted through a matrix external standard method. The fungal toxins can be completely and effectively separated within 9 min, the linear relation of the fungal toxins within separate linear response ranges is good, the correlation coefficient R<2> is larger than 0.999, the lowest limit of quantification is lower than 1.0 microgram / L, the lowest detection limit is lower than 0.1 microgram / L, the recovery rate ranges from 70% to 120%, and the relative standard deviation (RSD) ranges from 0.5% to 4%. Compared with the national standard method, the method has the advantages of being rapid, simple, convenient, economical, efficient, safe and the like and can be suitable for separation and quantitative determination of various fungal toxins in baijiu and original auxiliary material samples.

It is an object of the invention to improve the current QuEChERS technology. In one aspect of the invention, the buffer-salt powder mixture of the first stage of the QuEChERS process is compressed into a solid dosage form before it is used in the first stage of the QuEChERS process. Replacing the QuEChERS powders (the buffer-salt powder mixture of the first stage of the QuEChERS process) with solid aliquots eliminates the need for using centrifuge tubes for packaging the product.

The invention discloses a method for detecting pesticide residues in vegetables and fruits. The method adopts an improved QuEChERS kit method for carrying out sample pretreatment, and has the advantages of being simple and rapid, high in efficiency and labor-saving, good in reproducibility, less in reagent consumption, low in labor intensity, low in cost, environmentally-friendly, and the like; furthermore, a UPLC-MS / MS-Qtrap detection method is cooperatively used, so that the simultaneous screening and confirmatory analysis of the 59 pesticide residues can be realized, and the high throughput analysis and detection of the multiple pesticide residues can be further realized.

The invention discloses a method for screening a sweetening agent in milk and dairy products by applying ultra-high performance liquid chromatography-quadrupole electrostatic field orbit ion trap mass spectrometry, and belongs to the technical field of food safety inspection. An optimized QuEChERS method is used for preprocessing samples; the ultra-high performance liquid chromatography-quadrupole electrostatic field orbit ion trap mass spectrometry is applied for performing screening, a scanning mode of full-scanning / data-dependency collection and full-scanning / variable data-independent collection and full-scanning / full-ion fragmentation collection is adopted, a positive ion or negative ion mode is selected for scanning, and complete first-order and second-order spectra are obtained; Extract Finder 2.5 software is applied for processing the obtained first-order and second-order spectra, and information of a compound is extracted. Information in a built spectrum library and a tested object screening result are compared, and the composition of the sweetening agent in the milk and dairy products is ensured. An adopted mass spectrometer has the main advantages that the resolution is high, qualitative and quantitative results are accurate, sensitivity is high, and quality precision is high and can be applied to complex matrix analysis. The established screening method has the good application prospect in the field of food.

The invention discloses a method for detecting pigment in flavouring. The method comprises the following steps: 1) performing pre-treatment on a sample to obtain a loading buffer; and 2) using an UPLC-MS / MS method for measuring the loading buffer, and comparing the determination result of the loading buffer with a standard solution for analysis. The detection method can perform qualitative and quantitative analysis through a positive and negative ions switching mode, can simultaneously detect the positive and negative ions of the pigment, and solves the technical problems of single detection mode of current illegal pigment and limited detection types. a QuEChERS purifying method is used for removing grease in the sample, the complex pre-treatment process in the pigment detection can be effectively improved, the method is simple and rapid, compared with the detection method in the prior art, the analysis time is short, and the sensitivity is high, detection efficiency is completely increased, and the detection period is greatly shortened.

The invention discloses a detection method for rapidly screening various pesticides and biotoxins in an aquatic product. The detection method comprises the following steps: (1) preparing a standard solution; (2) carrying out QuEChERS extraction and purification of a sample; (3) carrying out concentration; and (4) carrying out analyzing by an ultra-high performance liquid chromatography high-resolution mass spectrometer. QuEChERS is used for pretreating the aquatic product, meanwhile, ultrahigh performance liquid chromatography quadrupole / electrostatic field orbitrap mass spectrometry (UPLC-Q Exactive Orbitrap HRMS is combined to conduct high-throughput measurement on 93 kinds of pesticide and 16 kinds of biotoxin residues in the aquatic product, and the method is sensitive, rapid, simple,accurate, time-saving, stable, high in practicability and capable of meeting the detection requirements of the current market.

The invention discloses a method for simultaneously measuring 28 kinds of pesticide residues with gas chromatography-triple quadrupole tandem mass spectrometry. The method includes the steps of A, sample pretreatment, namely weighing fresh tea leaf powder and pouring the same into a centrifuge tube, adding 1% of an acetonitrile acetic acid solution, adding NaCl after homogenated extraction, and performing full shock blending and centrifugal stratifying prior to taking clear liquid on the upper layer for standby use; B, purification and interior label treatment, namely pouring the clear liquid on the upper layer into the centrifuge tube, purifying the clear liquid with QuEChERS and adding an interior label solution, and taking supernate for standby use after whirlpools shock and centrifugation; C, mass spectrometric measurement, namely subjecting the supernate in the step B to qualitative and quantitative analysis with the gas chromatography-triple quadrupole tandem mass spectrometry. With the method, the problem about measurement blank of the pesticide residues of tea leaves is solved, and the method has the advantages of high flexibility, good selection and high repeatability and accuracy, the 28 kinds of pesticide residues in the green leaves can be measured simultaneously, and the method is specially suitable for measurement analysis for large amount of samples.

The invention discloses an ultra-high performance liquid chromatography-quadrupole static electric field orbitrap mass spectrometry screening method for mycotoxin in milk and dairy products. The method is characterized in that a sample is pre-treated by an optimized QuEChERS method; a ultra-high performance liquid chromatography-quadrupole static electric field orbitrap mass spectrometry is used for screening, full-scanning / data-independent collection, full-scanning / variable data non-independent collection, and full-scanning / ion fragmentation collection scanning mode is employed, a positive ion or negative ion mode is selected for scanning to obtain complete first-order and second-order spectrums; Exact Finder 2.5 software is used for processing the obtained first-order and second-order spectrums, and the compound information is extracted. Compared the information in spectrum database and the screening result of the to-be-measured objects, composition of mycotoxin in the milk and dairy products can be confirmed. The mass spectrum apparatus has the advantages of high resolution, accurate qualitative and quantification result, high sensitivity, and high quality precision, and can be used for analyzing the complex matrix.

The invention discloses a method for rapidly determining 14 mycotoxins and 26 pesticide residues in corn. According to the method of the invention, an extracting solution is adopted for extraction, the ratio of acetonitrile, water, and acetic acid in the extracting solution are 79: 20: 1; an QuEChERS extraction bag (containing MgSO4, NaCl, sodium citrate and disodium citrate) is used for purification; a UPLC-MS / MS method is adopted; a UPLC HSS T3 column is used as a chromatographic column; 0.2% formic acid water / methanol is used as a mobile phase so as to perform qualitative and quantitative analysis; the average recovery rate of all mycotoxins and pesticides ranges from 73.37% to 105.87%. The method is quite suitable for the detection of mycotoxins and pesticides in corn and has importantsignificance in guaranteeing the quality safety of corn products and maintaining human health.