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Sample purifying adsorbent used for detecting pesticide residues of fruits and vegetables, sample preprocessing method, and pesticide residue detection method

A technology for sample pretreatment and pesticide residue detection, applied in chemical instruments and methods, inorganic chemistry, measuring devices, etc., can solve the problems of limiting the speed of sample pretreatment, and achieve the effect of saving operation time, reducing loss and shortening time

Active Publication Date: 2017-04-26
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Compared with other sample pretreatment methods, this method is relatively fast, but multi-step centrifugation will still limit the sample pretreatment speed. Therefore, there is an urgent need for a sample pretreatment method with high processing efficiency and easy operation.

Method used

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  • Sample purifying adsorbent used for detecting pesticide residues of fruits and vegetables, sample preprocessing method, and pesticide residue detection method
  • Sample purifying adsorbent used for detecting pesticide residues of fruits and vegetables, sample preprocessing method, and pesticide residue detection method
  • Sample purifying adsorbent used for detecting pesticide residues of fruits and vegetables, sample preprocessing method, and pesticide residue detection method

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preparation example Construction

[0031] The present invention has no special limitation on the preparation method of the purification adsorbent, it only needs to mix the components described in the above technical solution according to parts by weight. The Fe contained in the purification adsorbent of the present invention 3 o 4 @SiO 2 @PSA is a kind of magnetic nanoparticle, so that the purification adsorbent can be adsorbed by the magnetic field, so the purification adsorbent provided by the present invention can be separated from solid and liquid without centrifugation after adsorption, the adsorption efficiency is high, and the steps of sample pretreatment can be simplified , saving operating time.

[0032] The present invention also provides a method for sample pretreatment when applying the purified adsorbent described in the above technical scheme to detect pesticide residues in fruits and vegetables, comprising the following steps:

[0033] (1) mixing the fruit and vegetable homogenate sample to be...

Embodiment 1

[0050] Crush and homogenize the green vegetables, weigh 2.00 g of the above-mentioned green vegetable homogenate sample, add azoxystrobin standard product, make the concentration of azoxystrobin in the sample to be tested be 100 μg / kg, and let it stand for 30 minutes. Weigh 2.00 g of the spiked green vegetable homogenate sample and mix with 2.00 ml of acetonitrile, vortex for 1 min to obtain the first mixture; mix with 0.35 g of sodium chloride and 1.4 g of anhydrous magnesium sulfate, and vortex for 1 min to obtain the second mixture ; The second mixture is mixed with 60mg purification adsorbent, and vortexed for 0.5min to obtain a magnetic adsorption mixture, and the purification adsorbent is composed of 10mg Fe 3 o 4 @SiO 2 Composed of @PSA and 50mg graphitized carbon black; under the action of an external magnetic field, the magnetic adsorption mixture is separated from solid and liquid, absorb 0.5ml supernatant extract and mix with 0.5ml water, filter with 0.22μm micropo...

Embodiment 2

[0054] Crush and homogenize the tomato, weigh 2.00 g of the tomato homogenate sample, add a standard triadimefon to make the concentration of triadimefon in the sample to be tested 100 μg / kg, and let it stand for 30 minutes. Weigh 2.00 g of the tomato homogenate sample after adding the standard and mix with 2.00 ml of acetonitrile, vortex for 1 min to obtain the first mixture; mix with 0.3 g of sodium chloride and 1.2 g of anhydrous magnesium sulfate, and vortex for 1 min to obtain the second mixture ; The second mixture is mixed with 10mg purification adsorbent, and vortexed for 0.5min to obtain a magnetic adsorption mixture, and the purification adsorbent is composed of 10mg Fe 3 o 4 @SiO 2 @PSA composition; under the action of an external magnetic field, the magnetic adsorption mixture is separated from solid to liquid, absorb 0.5ml of supernatant extract and mix with 0.5ml of water, filter with 0.22μm microporous membrane, and the obtained filtered solution is the sample ...

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Abstract

The invention provides a sample preprocessing purifying adsorbent used for detecting the pesticide residues of fruits and vegetables. The purifying adsorbent comprises, by weight, 0-50 parts of C18, 0-50 parts of graphitized carbon black and 10-50 parts of Fe3O4@SiO2@PSA. The purifying adsorbent can be used to preprocess a sample in order to combine extracting and purifying steps of preprocessing methods in the prior art into one steps, so the operating time is effectively saved, and a centrifuge step between the extracting step and the purifying step is omitted; and compared with existing wide-applied rapid preprocessing methods QuECHERS, a preprocessing method in the invention reduces the consumption time by 61.1%, and allows 50 samples to be simultaneously preprocessed in 70 min only.

Description

technical field [0001] The invention relates to the technical field of pesticide residue detection, in particular to a sample purification adsorbent used for detection of pesticide residues in fruits and vegetables, a sample pretreatment method and a pesticide residue detection method. Background technique [0002] The problem of pesticide residues arises with the mass production and widespread use of pesticides. Pesticides are not decomposed within a period of time after application and remain on fruits and vegetables. So far, the annual output of chemical pesticides in the world is nearly 2 million tons, and more than 1,000 synthetic compounds are used as pesticides such as insecticides, fungicides, algicides, insecticides, and defoliants. The large-scale application of pesticides, especially organic pesticides, has caused serious pesticide pollution problems and has become a serious threat to human health. Therefore, establishing a fast and accurate method for the determi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14B01J20/22B01J20/28
CPCB01J20/06B01J20/103B01J20/20B01J20/22B01J20/28009G01N30/02G01N30/06G01N30/14G01N2030/027G01N2030/062G01N2030/143G01N2030/146
Inventor 王新全齐沛沛王祥云刘真真汪志威徐霞红章虎徐浩王强
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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