55 results about "Complementation" patented technology

The invention relates to the technical field of plant genetic engineering, in particular to separation, cloning and an application of two genes named SaM and SaF of a rice hybrid pollen fertility gene locus Sa. The bacterial two-hybrid experiments indicate that proteins coded by specific allele of the SaM and SaF of indica and japonica are subjected to specific interaction. In addition, genetic analyses and transgene functional complementation experiments indicate that the SaM heterozygous in a hybrid and the SaF originated from the indica, namely 3 alleles, interact to lead to infertility of hybrid pollen, while lack of any one of the three or inhibition of expression thereof results in hybrid compatibility, namely pollen fertility. The invention also relates to a method for adopting the genes to nurture rice hybrid fertility compatible line and an application thereof and a molecule marker for appraising rice hybrid fertility gene type and the application thereof.

The invention discloses a paddy rice leaf color regulation and control gene YL1 and a use thereof. The paddy rice leaf color regulation and control gene YL1 has a gene nucleotide sequence shown in the formula of SEQ ID NO. 1. A coded protein sequence is shown in the formula of SEQ ID NO. 2. Through a map-based cloning technology, the novel paddy rice leaf color regulation and control gene YL1 is obtained. Through a transgenosis function complementation experiment, functions of the gene are verified. The YL1 and a chloroplast ATPase protein complex subunit AtpB produce synergism and YL1 gene mutation causes chloroplast ATPase activity reduction so that a photosynthesis-associated protein expression level is reduced, chloroplast development is influenced, chlorophyll content is reduced and a yellow leaf phenotype is produced. The protein and coding gene can further elucidate a paddy rice leaf color regulation and control molecule mechanism, can be used for crop genetic improvement and has an important meaning for high photosynthetic efficiency breeding.

The invention discloses a rice ageing-relevant control gene OsCDC48E sequence and a coded translated protein and an amino acid sequence thereof. Through a map-based cloning technology, a gene OsCDC48E for controlling rice leaf ageing is obtained from a rice premature senile mutant W95 through cloning, and functional complementation experiments prove that the OsCDC48E is a gene which controls the rice growth and development and is relevant to leaf ageing. Observation of a transmission electron microscope, and analysis of a photosynthetic rate and a photoreaction and dark reaction detection technology prove that a premature senile gene cloned from the OsCDC48E has a remarkable adjusting function of growth and development of rice, integrity of thylakoids in chloroplast, structures of organelle of endoplasmic reticulum, and the like. By using a genetic engineering technology, multiple specific marker characters of the ageing gene can be applied to rice variety improvement, yield increase and cultivation of super rice.

The invention belongs to the technical field of biology, and relates to short-chain dehydrogenase and a mutant thereof, and preparation and applications of a gene, specifically to a wild type C=C double bond reductase OYE1 derived from Saccharomyces pastoris, and a mutant thereof, short-chain dehydrogenases LfSDR1, BmSDR5 and BsSDR8 respectively derived from Lactobacillus fermentum, Bacillus megaterium and Bacillus subtilis, mutants thereof, C=C double bond reductase and C=O double bond reductase with stereoselectivity complementation, genes of C=C double bond reductase and C=O double bond reductase, construction and preparation of a recombinant expression vector containing the gene, expression of recombinase, an enzyme catalytic two-step asymmetric reduction reaction of carvone, the conditions of the reaction, and a detection method of the product dihydrocarveol. Compared with the existing chemical method, the method of the invention has the advantages of being easy to operate, mild in reaction condition, environmentally friendly and the like.

Use of mutated β-arrestin for an improved enzyme complementation assay or translocation assay, the improved enzyme complementation assay comprising: i) adding a substrate to a cell comprising a GPCR-EA fusion protein and a β-arrestin-EB fusion protein, wherein the β-arrestin is mutated, ii) adding a ligand to obtain, if possible, a GPCR-EA / β-arrestin-EB complex, and iii) measuring a signal arising from association of EA and EB to create an enzymatically active protein catalyzing conversion of the substrate which leads to a detectable signal, wherein the improvement leads to an increased signal compared with the signal obtained by use of the same process employing a β-arrestin-EB fusion protein, wherein the β-arrestin is wild type β-arrestin, and the improved β-arrestin translocation assay comprising i) providing a cell expressing a GPCR and comprising a β-arrestin associated with an optically detectable molecule, ODM, wherein the β-arrestin is mutated, ii) adding a ligand to obtain, if possible, a GPCR / β-arrestin complex, and iii) detecting a translocation of the optically detectable molecule, wherein the improvement leads to a increased and prolonged translocation of the β-arrestin associated with an optically detectable molecule as compared with the signal obtained by use of the same assay employing a β-arrestin associated with an optically detectable molecule, wherein the β-arrestin is wild type β-arrestin.

Provided here are methods for editing of a host genome to knock out or debilitate genes responsible for the growth and / or differentiation of a target organ and injecting that animal at an embryo stagewith donor stem cells to complement the missing genetic information for the growth and development of the organ. The result is a chimeric animal in which the complemented tissue (human / humanized organ) matches the genotype and phenotype of the donor. Such organs may be made in a single generation and the stem cell may be taken or generated from the patient's own body. As disclosed herein, it is possible to do so by simultaneously editing multiple genes in a cell or embryo creating a 'niche"' for the complemented tissue. Multiple genes can be targeted for editing using targeted nucleases and homology directed repair (HDR) templates in vertebrate cells or embryos.

The invention discloses a tyrosine phosphatase biosensor as well as a detection method and application thereof. The tyrosine phosphatase biosensor comprises magnetic beads, a peptide-DNA conjugate, aDNA template and a signal probe, wherein one end of a peptide chain in the peptide-DNA conjugate is linked to a 5' end of a first DNA sequence, the peptide chain contains phosphorylated tyrosine, theother end of the peptide chain is used for linking the magnetic beads; the DNA template consists of two second DNA sequences and a third DNA sequence from a 5' end to a 3' end, the third DNA sequencecan be complemented with a first DNA sequence, the DNA template is hybridized and polymerized with the first DNA sequence so as to form double-chain DNA with two nickase recognition sites, and complementary chains of the second DNA sequences are dnazyme; and the signal probe is a nucleotide sequence, fluorophores and quenchers are respectively linked to two ends of the nucleotide sequence, and thenucleotide sequence can be complemented with the dnazyme; and the dnazyme is an RNA sequence capable of realizing cracking and complementation in dependent reaction of divalent cations

The invention relates to the plant genetic engineering field, in particular to clone of a pleiotropic gene Ghd8 which affects yield, florescence and plant height of rice grain. In the invention, the gene Ghd8 is separated and cloned by experiments such as establishment of a near-isogenic line of a target gene, preliminary location, comparative sequencing, genetic transformation and complementation and the like, wherein the sequence of the gene Ghd8 is shown as SEQ ID NO:2. Compared with a recessive allelic single plant, the yield of a dominant allelic single plant is increased by 58%, the plant height thereof is increased by 25.6%, but the heading stage thereof is only increased by 9 days. By means of results of Ghd8 main mutation site and trait correlation analysis and haploid cluster analysis of protein sequences of different varieties, the near-isogenic lines of different allelic types are established to obtain four favorable Ghd8 allelic types, wherein, the Ghd8-9311 and Ghd8-ruf allelic types can increase yield of the rice grain but not delay blossoming, thus being applicable to breeding the rice grain in areas with good light and temperature conditions; and Ghd8-MH63 and Ghd8-Nip allelic types are not photosensitive, thus being applicable to increasing yield and breeding the rice grain in areas under a short-day condition.

The invention discloses an application of a Nat1 gene as a selection marker in genetic transformation of oomycetes. The invention discloses an antibiotic, namely nourseothricin as a selection marker in transformation of oomycetes. According to the invention, a coding gene Nat1 capable of causing the resistance of oomycetes to nourseothricin is used as a selection marker for screening transformants during phytophthora sojae transformation. Development of the selection marker solves the problem of single selection marker during transformation of oomycetes, and provides powerful guarantee for gene complementation, gene knockout, etc., during transformation of oomycetes.