481 results about "Heme" patented technology

Methods and compositions for identifying ovarian cancer in a patient sample are provided. The methods of the invention comprise detecting overexpression of at least one biomarker in a body sample, wherein the biomarker is selectively overexpressed in ovarian cancer. In preferred embodiments, the body sample is a serum sample. The biomarkers of the invention include any genes or proteins that are selectively overexpressed in ovarian cancer, including, for example, acute phase reactants, lipoproteins, proteins involved in the regulation of the complement system, regulators of apoptosis, proteins that bind hemoglobin, heme, or iron, cytostructural proteins, enzymes that detoxify metabolic byproducts, growth factors, and hormone transporters. In some aspects of the invention, overexpression of a biomarker of interest is detected at the protein level using biomarker-specific antibodies or at the nucleic acid level using nucleic acid hybridization techniques. Kits for practicing the methods of the invention are further provided.

Cytochrome b5 (Cb5) is a haem-binding protein located in the endoplasmic reticulum (ER) and the outer mitochondrial membranes of higher eukaryotes. In higher plants, animals, and fungi, the ER resident Cb5 has been shown to play a role in desaturation of acyl CoA fatty acids. Higher plants Cb5 isoforms from plants such as soybean or Arabidopsis are capable of modulating omega-3 desaturation. Co-expression of certain Cb5 isoforms with FAD3 in a host plant results in increased production of seed oil content as well as altered ratio between different fatty acids. It is also disclosed here that overexpression of Yarrowia ACL enzymes in the plastids of a host plant helps boost the synthesis of acetyl CoA, which in turn, may lead to increased synthesis of fatty acids and enhanced oil accumulation in the seeds.

Compounds of Formula (IA) and Formula (IB) are described. They are useful as stimulators of sGC, particularly NO-independent, heme-dependent stimulators. These compounds may be useful for treating, preventing or managing various disorders that are herein disclosed.

The invention relates to novel variants of cytochrome P450 oxygenases. These variants have an improved ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. These variants also have an improved thermostability as compared to the cytochrome P450 BM-3 F87A mutant. Preferred variants include cytochrome P450 BM-3 heme domain mutants having I58V, F87A, H100R, F107L, A135S, M145A / V, N239H, S274T, L324I, I366V, K434E, E442K, and / or V446I amino acid substitutions.

Disclosed herein are hemojuvelin-specific siRNAs that vary hemojuvelin mRNA concentration. Also disclosed herein, GPI-hemojuvelin positively regulated hepcidin mRNA expression, independently of the IL-6 pathway, whereas soluble hemojuvelin (s-hemojuvelin) suppressed hepcidin mRNA expression in primary human hepatocytes in a log-linear dosedependent manner. Disclosed are compositions and methods for modulating diseases of iron metabolism and hepcidin expression or hepcidin levels.

Compound of Table I are described. They are useful as stimulators of sGC, particularly NO-independent, heme-dependent stimulators. These compounds may be useful for treating, preventing or managing various disorders that are herein disclosed.

An optical tomography system has a group of lasers for generating light of multiple distinct wavelengths within a first wavelength band. Light from these lasers is directed into mammalian tissue at laser-specific locations on the tissue, and light from the mammalian tissue is collected at a plurality of reception points. Collected light from each reception point is separated according to its wavelength, and received by a photodetector to produce path attenuation signals representing attenuation along paths between the laser-specific locations on the tissue and the reception points. Image construction apparatus generates a tomographic image of heme concentrations in the mammalian tissue from the path attenuation signals. In an alternative embodiment, there is a second group of lasers operating in a second wavelength band, and the image construction apparatus can generate an image of heme oxygenation In the mammalian tissue.

The invention provides a probe based on a G-quadruplex-chlorine heme DNA enzyme and a method for detecting protein. The method for detecting the protein facilitates detecting operation and is low in cost and free of marking. The probe has the advantages of being high in sensitivity and low in background noise. When the protein is detected, the detecting operation is easy, consumed time is short, cost is low, and the problems that a method for detecting the protein in the prior art is long in consumed time, high in cost and complex in detecting probe manufacturing are solved.

Compounds of Formula I are described. They are useful as stimulators of sGC, particularly NO-independent, heme-dependent stimulators. These compounds may be useful for treating, preventing or managing various disorders that are herein disclosed.

The invention relates to an electrochemical method of detecting single-chain target DNA concentration based on G-quadruplex-heme compound and polymeric chain type amplification reaction, and belongs to the technical field of analytical chemistry. A capture probe and an auxiliary probe are designed, the two ends of the auxiliary probe each contain a nucleotide sequence complemented with the target DNA, and the middle of the auxiliary probe contains a base sequence capable of forming G-quadruplex. The capture probe and the target DNA recognize each other and are subjected to continuous polymeric chain type reaction to form chain-shaped polymer, the chain-shaped polymer is fixed to an electrode through the capture probe on the surface of the gold electrode, and a great number of G-quadruplex structures are introduced onto the surface of the electrode. Then, G-quadruplex and heme are combined to form the compound with powerful electrochemical signals, and the target DNA is detected through the corresponding relation among the electrochemical signals obtained through differential pulse voltammetry (DPV) scanning, the G-quadruplex-heme compound on the surface of the electrode and the concentration of the target DNA added into the system. HIV DNA in the sample is detected through the method, and an ideal effect is obtained. The electrochemical method has the advantages of being high in sensitivity and specificity.

The invention relates to a culture medium for Haemophilus influenzae type b, characterized in that the source of protein nitrogen is of non-animal origin and comprises at least one plant peptone and in that the heme source consists of protoporphyrin IX. This medium serves in particular for the production of polyribosyl phosphate (PRP) and for the manufacture of a vaccine against Haemophilus influenzae type b meningitis.

Methods and compositions for identifying ovarian cancer in a patient sample are provided. The methods of the invention comprise detecting overexpression of at least one biomarker in a body sample, wherein the biomarker is selectively overexpressed in ovarian cancer. In preferred embodiments, the body sample is a serum sample. The biomarkers of the invention include any genes or proteins that are selectively overexpressed in ovarian cancer, including, for example, acute phase reactants, lipoproteins, proteins involved in the regulation of the complement system, regulators of apoptosis, proteins that bind hemoglobin, heme, or iron, cytostructural proteins, enzymes that detoxify metabolic byproducts, growth factors, and hormone transporters. In some aspects of the invention, overexpression of a biomarker of interest is detected at the protein level using biomarker-specific antibodies or at the nucleic acid level using nucleic acid hybridization techniques. Kits for practicing the methods of the invention are further provided.

The invention relates to novel variants of cytochrome P450 oxygenases. These variants have at least one mutation improving their ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. The variants also have at least one mutation improving thermostability as compared to the parent enzyme or corresponding wild-type enzyme. Preferred variants include cytochrome P450 BM-3 heme domain variants having L52I, I58V, F87A, H100R, S106R, F107L, A135S, M145A / V, A184V, N239H, S274T, L324I, V340M, I366V, K434E, E442K, and / or V446I amino acid substitutions.

The invention belongs to the field of metal single atom doped carbon material, and especially relates to a simple method used for preparing high loading capacity metal single atom catalytic materials.According to the simple method, nitric acid functionalized carbon tube graphene is taken as a carrier; carbon resources and nitrogen resources such as urea, dicyandiamide, and melamine which are capable of generating C3N4 in pyrolysis process are taken as single atom dispersion templates; metal organic salts such as heme and phthalocyanines are taken as precursors; at inert atmosphere, multi-steppyrolysis is adopted to prepare a series of loaded metal single atom catalytic materials. The method is capable of solving problems in the prior art that single atom metal loading capacity is low, and dispersibility is poor. The single atom catalytic materials synthesized using the method possess excellent oxidation reduction performance at acidic conditions, can be taken as proton exchange membrane fuel cell cathode and anode materials, and are capable of reducing proton exchange membrane fuel cell catalyst cost.

A transdermal patch for the treatment of iron deficiency including a drug reservoir layer containing an hematinic substance; a rate-controlling membrane secured to said reservoir layer; and a contact adhesive secured to said rate-controlling membrane, wherein said hematinic substance is selected from the class consisting of ferrous sulfate, ferrous lactate, ferrous iodide, ferrous gluconate, ferrous fumarate, ferrous citrate, ferrous carbonate saccharated, ferrous carbonate mass, ferronascin, ferroglycine sulfate, and ferrocholinate.

Fusion proteins comprising a first protein fused to hemopexin are provided, said fusion proteins exhibit an increased circulation time.

The present invention relates to combination therapies for heme malignancies with anti-CD38 antibodies and survivin inhibitors.

A method for treating helminthic infections in a mammal or plant which entails administering one or more compounds which are metal-ligand chelate compounds containing a metal and a tetrapyrrole compound or a porphyrin compound, to mammal or plant in need thereof.

The invention is directed to vaccine compositions and methods based on P. gingivalis proteins identified to be regulated by haem availability that can be used in the prevention and treatment of periodontal disease. In particular, two specific internalin-like P. gingivalis proteins, namely PG0350 and PG1374 involved in the internalization of P. gingivalis by host cells, the hypothetical protein, PG1019 purported to be a cell surface lipoprotein and the alkyl hydroperoxide reductase protein, PG0618 have been identified as useful targets for the prevention and treatment of periodontal disease.

Provided herein are microorganisms engineered with heme-responsive transcription factors and genetic circuits. Also provided are methods for using engineered microorganisms to sense bleeding events and treat bleeding in vivo.

Zero valent metal composite, manufacturing thereof, using thereof, and system including thereof, for (in-situ or ex-situ) catalytically treating contaminated water, such as sub-surface water, surface water, above-surface water, water vapor, or / and gaseous water. Composite includes powdered diatomite matrix incorporated with nanometer (1-1000 nm) sized particles of a zero valent (transition) metal (iron, cobalt, nickel, copper, zinc, palladium, platinum, or / and gold) and at least one electron transfer mediator (catalyst) from porphyrinogenic organometallic complexes (e.g., metalloporphyrins (chlorophylls, hemes, cytochromes) or metallocorrins (e.g., vitamin B12), and optionally, includes vermiculite. System includes composite and in-situ or / and ex-situ unit containing the composite, enabling exposure of contaminated water thereto. Applicable to in-situ sub-surface permeable reactive barriers (PRBs). Treatable water contaminants are organics (halogenated organic compounds), or / and inorganics (metal elements, metal element containing inorganic species, nonmetal elements, and nonmetal element containing inorganic species). Applicable to non-aqueous fluids (liquids, vapors, gases), for removing contaminants therefrom.

The invention discloses a copper ion detection kit based on click chemistry and G tetramer with HRP (Horse Radish Peroxidase) activity and a detection method of the copper ion detection kit. A G tetramer formation sequence is formed by two DNA (deoxyribonucleic acid) sequences modified with an azide group and an alkynyl group through click chemistry in a reaction system, and then ferriheme and KCl are added to form a ferriheme / G tetramer structure. The ferriheme / G tetramer has the HRP activity and can catalyze a colourless substrate TMB (tetramethylbenzidine) to form a colored substrate, and the experiment result can be judged by eyes. When quantitative analysis is performed, the final reaction solution is transferred to an elisa plate, and the OD (optical density) value of the solution under 450nm can be read by an elisa meter. The copper ion detection kit can be used for performing qualitative and quantitative analysis of concentration of Cu<2+>, the detection system is simple, the specificity is good, the detection is not affected by other substances, the operation is easy, the reaction is fast, the detection result is reliable, and the copper ion detection kit is suitable for local laboratories.

The production of serum peptide hormone by the hydrolysis of pig blood with a compound enzyme method belongs to the biochemical technology field. The invention relates to the expansion of the space structure of globulin and the breaking and hydrolysis of a molecular chain, the main processes are as follows: red blood cells are extracted from fresh blood in a centrifugal way and then swelled and mixed with plasma; later pH2-3 is adjusted, and 95% of ethanol and 2.8% of H2O2 with equivalent weight are added; next, sodium hydroxide is added with the heat preserved for 2h at 80 DEG C; pH8 is adjusted, and papain, trypsin, alkaline protease and the like are added with 4h of enzymolysis at 37 DEG C and then pH3.0 is adjusted; after pepsin is added with 4h of enzymolysis at 37 DEG C, a serum peptide hormone product is obtained by enzyme destroying and drying. The technology comprises the expansion of internal gene of the globulin, the oxidation of heme, the hydrolysis of compound enzyme and the like, has over 90% of hydrolysis rate, and solves the problems of difficult hydrolysis of blood globulin and complex process in the conventional process; the serum peptide hormone product which is produced by the invention has over 60% of peptide content, simultaneously preserves the AgI immune function factors and the like in blood, enhances the organism immunity function, and is suitable for blood comprehensively processing such as slaughterhouses, etc.

A method and device for processing mammalian adipose tissue such that the vascular rich fraction is separated from the vascular poor fraction, Mammalian adipose tissue in the form of morselated surgical biopsies and / or lipoaspirate from liposuction is placed within a novel syringe attached to a detection device measuring either color, light saturation, infra-red light, heme, iron or oxygen saturation. This process involves no label and minimal manipulation and handling of the tissue. This process and device may also be used intra-operatively under sterile conditions for immediate use within the same individual receiving liposuction or surgery.

The invention discloses a carbon point-protoheme based analogue peroxide enzyme composite nanometer material, and builds a method for visually detecting hydrogen peroxide, glucose and xanthine via visual and fluorescence dual channels by using the nanometer material. According to the method, a stable red color development product is formed by catalyzing hydrogen peroxide phenol oxide and 4-amino-antipyrine by carbon point-protoheme analogue enzymes; the maximum absorption peak of the color development product is 505nm; meanwhile, the fluorescence of the reaction liquid of the carbon point-protoheme nanometer material in a position with the excitation wavelength being 480 nm and the emission spectrum being 540nm generates quenching. Therefore the light absorption degree of color developmentproduct in the 505nm position and the fluorescence quenching signals of the color development product in the 540nm emission spectrum position respectively realize the linear relationship with hydrogen peroxide, glucose and xanthine in a range of 1.7*10<-7> to 2*10<-4> M, 1.7*10<-7> to 1.3*10<-4> M and 3.3*10<-6> to 3.3*10<-5>M.

Compounds of Formulae I′ and I are described, which are useful as stimulators of sGC, particularly NO-independent, heme-dependent stimulators. These compounds are also useful for treating, preventing or managing various disorders that are herein disclosed.

The invention discloses a method for comprehensively utilizing porcine hemoglobin. The method comprises the steps that complete hemoglobin is subjected to enzymolysis; an enzymatic hydrolysate is heated and treated with salt at a certain concentration in an alkaline solution to facilitate protoheme to be dissociated from polypeptide; and proteome is separated from polypeptide and small peptide by ultrafiltration and nanofiltration methods, so that the purpose of comprehensively utilizing porcine hemoglobin is finally achieved. The method is significant in economic benefit, and high in social and environmental protection values; the method does not use organic solvent, so that the process is simplified, the cost is lowered, and no waste is generated; protoheme is separated from polypeptide and small peptide thoroughly, so that the yield and the purity of a product are high; and the industrialization application prospect is broad.

This invention provides novel assays for the detection of dysfunctional HDL. The assays are good diagnostics and / or prognostics for atherosclerosis or other pathologies characterized by an inflammatory response. In certain embodiments the methods involve measurements of heme-related HDL-associated proteins (e.g., haptoglobin, hemopexin, etc.), and / or measurements of the relative distribution of HDL-associated proteins between HDL and the non-lipoprotein fractions of plasma / serum, and / or measurements of the ability of pro-inflammatory HDL to consume nitric oxide, and / or measurement of the ability of HDL to inhibit LDL aggregation.