The invention relates to a genetically modified prokaryotic
cell capable of improved iron-
sulfur cluster delivery, characterized by a modified
gene encoding a
mutant Iron
Sulfur Cluster
Regulator (IscR) and one or more transgenes or upregulated endogenous genes encoding iron-
sulfur (Fe—S) cluster polypeptides or proteins that catalyze complex radical-mediated molecular rearrangements,
electron transfer, radical or non-
redox reactions,
sulfur donation or perform regulatory functions. The
prokaryotic cells are characterized by enhanced activity of these iron-sulfur (Fe—S) cluster polypeptides, enhancing their respective functional capacity, and facilitating enhanced yields of compounds in free and
protein-bound forms, including
heme, hemoproteins, tetrapyrroles,
B vitamins, amino acids, δ-aminolevulinic acid, biofuels, isoprenoids,
pyrroloquinoline quinone,
ammonia,
indigo, or their precursors, whose
biosynthesis depends on their activity. The invention further relates to a method for producing said compounds or their precursors using the genetically modified prokaryotic
cell of the invention, and the use of the genetically modified prokaryotic
cell.