A method of, and
system for, assaying for selected constituents in liquid mixtures confined by corresponding containing structures having relatively small
electromagnetic absorption in at least one transmission
wavelength range for transmissions of
electromagnetic radiation therethrough, including
in vivo assaying for a presence of selected constituents in bloodstreams in
circulatory system passageways in mammalian bodies having relatively small
electromagnetic radiation absorption in at least one transmission
wavelength range for transmissions of
electromagnetic radiation between such passageways and outer surfaces of corresponding bodily
skin, based on, in the bloodstream example, introducing in a bloodstream a probe comprising a binding base capable of binding to at least one of the selected constituents and of also concurrently conjugating to two different fluorophores of which one has an emission spectra peak distribution in the transmission
wavelength range that overlaps an absorption spectra peak distribution of that one remaining. Radiate at least some of the passageways from at least one location on the outer surfaces of the corresponding
skin with electromagnetic
radiation of wavelengths in the transmission
wavelength range and also in an absorption spectra range of that
fluorophore having an overlapping emission spectra peak distribution. Detect electromagnetic
radiation at least at one location on the outer surfaces of the corresponding
skin that has been transmitted from the evaluative radiated passageways of wavelengths in the transmission
wavelength range and also in an emission spectra range of that
fluorophore having an overlapping absorption spectra peak distribution wherein the emission spectra range differs from the overlapping emission spectra peak distribution.