The invention relates to a preparation method of a
phycoerythrin ratio
fluorescence sensor based on a magnetic molecular imprinted
nucleus / shell
polymer. A Fe3O4 magnetic nano-particleis used as the center, the surface of the Fe3O4 magnetic nano-particleis coupled with blue
fluorescence emitting
carbon quantum dots B-CDs, a SiO2 shell layer loaded with template molecule
phycoerythrin generates onthe surfaces of the Fe3O4 / B-CDs, and template molecule
elution is conducted to prepare magnetic molecular imprinted
nucleus / shell
polymer Fe3O4 / B-CDs / SiO2-MIPs. The
polymer dispersion liquid
fluorescence emission spectra under different
phycoerythrin concentration are measured, the
linear relation between the fluorescence emission
peak intensity ratio I<phycoerythrin> / I<B-CDs> of the phycoerythrinto the B-CDs and the phycoerythrin
molar concentration is fitted, and a phycoerythrin ratio
fluorescence sensor is constructed. Compared with the prior art, the method is simple to operate and low incost, raw materials are easy to obtain, ratio
signal interference resisting capacity is high, accuracy is high, flexibility and selectivity are high, and a novel ratio
fluorescence sensor can be developed and used for efficient phycoerythrin detection.