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76 results about "Low ionic strength" patented technology

HSA-free formulations of interferon-beta

InactiveUS6887462B2Improve solubilityIncreasing the amount of monomeric IFN-βPowder deliveryNervous disorderSolubilityLow ionic strength
Stabilized pharmaceutical compositions comprising substantially monomeric interferon-beta (IFN-β) and methods useful in their preparation are provided. The compositions comprise the IFN-β solubilized in a low-ionic-strength formulation that maintains the composition at a pH of about 3.0 to about 5.0. Methods for preparing these compositions, and for increasing solubility of IFN-β in pharmaceutical compositions, are provided.
Owner:GLAXOSMITHKLINE BIOLOGICALS SA

Chromatographic method for high yield purification and viral inactivation of antibodies

An improved process for the purification of antibodies from human plasma or other sources is disclosed. The process involves suspension of the antibodies at pH 3.8 to 4.5 followed by addition of caprylic acid and a pH shift to pH 5.0 to 5.2. A precipitate of contaminating proteins, lipids and caprylate forms and is removed, while the majority of the antibodies remain in solution. Sodium caprylate is again added to a final concentration of not less than about 15 mM. This solution is incubated for 1 hour at 25° C. to effect viral inactivation. A precipitate (mainly caprylate) is removed and the clear solution is diluted with purified water to reduce ionic strength. Anion exchange chromatography using two different resins is utilized to obtain an exceptionally pure IgG with subclass distribution similar to the starting distribution. The method maximizes yield and produces a gamma globulin with greater than 99% purity. The resin columns used to obtain a high yield of IgG retain IgM and IgA. IgA and IgM may be eluted from these resins in high yield and purity.
Owner:BAYER HEALTHCARE LLC

Simultaneous Quantitative Determination of Multiple Antibiotics at One Time

InactiveCN102269747ASolve the problem of difficult separation of peaks with similar peak timesAvoid errorsComponent separationAnalysis dataLow ionic strength
The present invention relates to a method for one-time simultaneous quantitative determination of multiple antibiotics, comprising: (1) preparing a sample filtrate; (2) setting liquid chromatography conditions and mass spectrometry conditions; (3) using the above liquid chromatography conditions and mass spectrometry conditions to determine For the sample filtrate prepared in step (1), analyze the data to determine the type and content of antibiotics in the analyzed sample. The present invention can simultaneously detect multiple antibiotics in one experiment and quantify the detected antibiotics at the same time. The combination of liquid chromatography-mass spectrometry not only solves the problem of difficult separation of peaks with similar peak times in liquid chromatography, but also overcomes the The error caused by the inconspicuous peak of low ion current intensity in the mass spectrum is eliminated; the invention can be widely used in the detection and quantitative analysis of antibiotics in various samples such as feces, soil, water, food, and the like.
Owner:DONGHUA UNIV

Method for extracting fibrillin from chicken

The present invention discloses a method to extract myofibril protein from chicken, aiming to provide a method to extract myofibril protein with high purity, simplicity and low cost. The procedures are as follows: chicken under room temperature is cut into pieces, added with a low-ionic-strength extract with the pH of 6.0-7.0 to dissolve the dissolvable under the condition of low ionic acid strength, the solution undergoes even centrifuge to obtain a deposit containing myofibril protein, which is added with a high-ionic-strength extract with the pH of 6.0-7.0, the solution undergoes even centrifuge, and the separated myofibril protein deposit undergoes cycles of extraction with the high-ionic-strength extract, each of which is followed by centrifuges to eliminate the foreign proteins and the other impurities from the chicken, the deposit obtained from centrifuged suspension is the myofibril protein, which is added with the high-ionic-strength extract to prepare a solution, which is dialyzed under room temperature, frozen and dried to obtain the intended product of myofibril protein.
Owner:TIANJIN UNIV OF COMMERCE

Method for nucleic acids isolation

The present invention provides a method for isolating nucleic acids. The method comprises: contacting a sample containing nucleic acids with a solid phase in a first aqueous solution to provide a loaded solid phase; washing the loaded solid phase with a second aqueous solution to provide a washed solid phase; and eluting the washed solid phase with a low ionic strength liquid to obtain the isolated nucleic acids. The present invention also provides a kit for practicing the present method.
Owner:PROGENTECH +1

Convergent detection type erythrocyte blood type irregular antibody detection kit based on solid-phase agglutination technology and preparation method thereof

The invention relates to a convergent detection type erythrocyte blood type irregular antibody detection kit based on a solid-phase agglutination technology and a preparation method thereof, and belongs to the technical field of kit preparation. The kit comprises (1) a convergent detection type solid-phase agglutination reaction micro-plate; (2) a low-ion-strength solution; (3) an indication system; (4) negative control serum; and (5) positive control serum. The preparation method comprises the following steps of (1) preparing the convergent detection type solid-phase agglutination reaction micro-plate; (2) preparing the low-ion-strength solution; (3) preparing the indication system; (4) preparing the negative control serum; and (5) preparing the positive control serum; and through innovative utilization of high specificity adsorption of the erythrocyte by virtue of a monoclonal antibody, a freeze-drying preservation technology and a high-sensitivity two-step method indication system,the problems of low sensitivity, difficulty in storing the erythrocyte, easiness in leak detection and the like in an existing clinically irregular antibody detection method are practically solved, and the high-cost-performance detection kit is provided for the clinical front-line detection work.
Owner:广州血液中心
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