65results about How to "Reduce ionic strength" patented technology

Genetically-encodable, environmentally-responsive fusion proteins comprising ELP peptides. Such fusion proteins exhibit unique physico-chemical and functional properties that can be modulated as a function of solution environment. The invention also provides methods for purifying the FPs, which take advantage of these unique properties, including high-throughput purification methods.

A method of cleaning a soiled load in an automatic dishwasher comprising the step of contacting the load with a phosphate free wash liquor comprising exfoliated nanoclay, the liquor having a pH of from about 9 to about 12 and an ionic strength of from about 0.001 to about 0.02 moles / l.

This invention provides nanowire based molecular sensors and methods for detecting analytes in a microfluidic system. Methods for sensing analytes include detecting changed electrical parameters associated with contact of a nanowire with the analyte in a microfluidic system. Sensors of the invention include nanowires mounted in microchambers of a microfluidic system in electrical contact with the detector, whereby electrical parameter changes induced in the nanowire by the analyte can be monitored by the detector.

The invention relates to a purification method for sodium hyaluronate, comprising the following steps of: performing adsorption and filtration on comb enzymolysis solution once by active carbon, uniformly stirring and mixing the obtained filtrate with CPC (cetylpyridinium chloride) aqueous solution, performing complex-precipitation thoroughly, standing the precipitate, removing the supernatant, and stirring and dissociating the complex precipitate by dissociating solution; diluting the dissociated liquid to 2-8 folds of the volume thereof, performing complex-precipitation for the second time, removing the supernatant after the complex-precipitation is finished, washing the precipitate for 1-3 times, and stirring and dissociating the complex precipitate by the dissociating solution; and adsorbing the liquid dissociated for the second time by the active carbon, and then removing carbon and filtering, precipitating the filtrate by alcohol, dehydrating the obtained sodium hyaluronate, and vacuumizing. The sodium hyaluronate finished product prepared by the method is low in absorbance, few in impurities, high in yield, good in the physicochemical property of intermediate, and few in adverse reactions.

The invention provides a detoxification method of wood fibre hydrolysis sugar liquid, which takes prehydrolysis sugar liquid concentration solution of the dilute sulphuric acid pretreatment cornstalks as raw materials. The invention is characterized in that the mixture which is prepared by trialkylamine, n-caprylic alcohol and kerosene is taken as complexing extracting agent; and carrying out complexing extraction and detoxification on the concentrated prehydrolysis sugar liquid under the condition of room temperature. In the concentrated prehydrolysis sugar liquid after complexing extraction, the removal rate of the main inhibitor acetic acid exceeds 70%; the removal rate of hydromethyl furfural exceeds 40%; the removal rate of furfural reaches 100%; and simultaneously, part of the lignin degradation products are removed.

The invention discloses a method for adsorbing and removing phosphates from a water body by using zirconium oxide modified carbon nano tubes. The method comprises the following steps of: ultrasonically dispersing the pretreated carbon nano tubes into a zirconium oxide solution; modifying the carbon nano tubes with zirconium oxide by using a hydrothermal synthesis method to prepare the zirconium oxide modified carbon nano tubes; adding the zirconium oxide modified carbon nano tubes into a phosphorus water body, so as to adsorb and remove the phosphates from the water body. According to the method, the zirconium oxide modified carbon nano tube is used as an adsorbers for the first time and is applied to treatment of the phosphorus water body. The adsorber has a good effect of removing phosphates and is acid and alkali resistant; the adsorption time is short; the treatment condition is mild; and the treatment efficiency is high. The method creates good economical and environmental benefits when used for removing phosphates from a water body.

The invention discloses a method for preparing a water-soluble phytosterol nano-dispersion system with an intestinal targeting release function. The method comprises the following steps: preparing a phytosterol solution; preparing a pectin-containing ethanol water; adding zein into the pectin-containing ethanol water to obtain a zein pectin solution; mixing the phytosterol solution and the zein pectin solution by adopting an ultrasonic treatment mode to obtain a nano-particle dispersion liquid; performing rotary evaporation on the nano-particle dispersion liquid to remove organic solvent and water to obtain a zein / pectin-loaded phytosterol nano-dispersion system. According to the method, the ultrasonic method and anti-solvent method are adopted, the pectin ingredient is added based on thezein serving as an embedding medium, formation of a compact interface structure is further promoted on the basis of ensuring effective embedding of the zein on the phytosterol, and the stability of the nano-dispersion system is improved.

A method is used in a time-of-flight mass spectrometer for analysis of a first pulsed ion beam, the ions of which are disposed along the pulse direction, separated with respect to their ion masses. The ions of at least one individual predetermined ion mass or of at least one predetermined range of ion masses can be decoupled from the first pulsed ion beam, as at least one decoupled ion beam, and the first ion beam and the at least one decoupled ion beam are analyzed.

Methods of enhancing efficiency of downstream chromatography steps for purification of proteins comprising: (a) passing a composition comprising a polypeptide of interest and various contaminants through an ion exchange membrane, wherein the polypeptide and the membrane have opposite charge, at operating conditions comprised of a buffer having a pH sufficiently distinct from the pi of the polypeptide to enhance the charge of the polypeptide and a low ionic strength effective to prevent the shielding of charges by buffer ions, which cause the membrane to bind the polypeptide and at least one contaminant, (b) overloading the ion exchange membrane such that at least one contaminant remains bound to the membrane while the polypeptide of interest is primarily in the effluent; (c) collecting the effluent from the ion exchange membrane comprising the polypeptide of interest; (d) subjecting the membrane effluent comprising the polypeptide of interest to a purification step of similar charge as the previous membrane, and (e) recovering the purified polypeptide from the effluent of the charged ion exchange chromatography purification step.

The invention relates to a synthesis method of bimodal mesoporous silicon dioxide, belonging to the technical field of inorganic porous materials. The method is characterized in that by using a cationic quaternary ammonium salt surfactant as a template, a silicon source is subjected to prehydrolysis treatment in a phosphoric acid system incapable of easily forming ordered mesoporous phase, and a phosphate solid which is weakly alkaline after being dissolved is directly added to induce the synthesis of the bimodal mesoporous silicon dioxide, of which the specific area is 949.7-1181.8 m<2> / g, the pore volume is 0.68-1.05 cm<3> / g and the framework pore sizes are respectively 2.1-2.6nm and 3.3-3.9nm. The synthesis system and preparation conditions used by the method are beneficial to in-situ packaging of various metal ions in different mesoporous pore canals and also beneficial to in-situ coating and immobilization of bioactive guest molecules in different pore canals, so that the method has important application prospects in the field of development of novel commercial catalysts, biocatalysts, multistage porous complex function materials and the like.

The invention relates to the field of wet metallurgy and chemical industry and particularly discloses a method for extracting nickel, cobalt and copper from a nickel-copper alloy chloride leaching solution. The method includes the steps that (1) the chloride leaching solution is heated, the pH value of the solution is adjusted by slowly adding liquid ammonia under the stirring condition, and the solution is filtered so as to obtain a filtrate; (2) the saponification rate is controlled through a 2-ethylhexyl phosphate mono-2-ethylhexyl ester and a sulfonated kerosene extraction agent, and cobalt and copper ions in the filtrate in the step (1) are extracted; (3) cobalt in a loaded organic phase in the step (2) is washed with diluted hydrochloric acid, and copper in the organic phase obtainedafter cobalt washing is finished is back-extracted through diluted sulphuric acid; (4) an ammonium hydrogen carbonate solution is reacted with an extraction raffinate in the step (2), the reaction endpoint is controlled, and nickel powder is obtained through reduction; (5) the ammonium hydrogen carbonate solution is reacted with a cobalt chloride washing solution in the step (3), the reaction endpoint is controlled, and cobalt powder is obtained through reduction; and (6) the copper sulfate back extraction solution in the step (3) is evaporated and crystallized, and then copper sulfate pentahydrate is obtained.