3076 results about "Trehalose" patented technology

Novel trehalose click polymers have in vitro and in vivo application in the cellular delivery of biologically active molecules, including nucleic acids and polypeptides. The trehalose click polymers of the present invention provide increased stability in serum as compared with other non-viral vectors, and are particularly useful in protecting nucleic acids against degradation.

The invention provides a cell cryo-preserved liquid, an application, and an immune cell cryo-preservation method. The cell cryo-preserved liquid includes 0.7-0.9 ml / ml of a cell culture medium, 8-26 mg / ml of non-essential amino acids, 0.04-0.1 ml / ml of trehalose, 0.5-10 mg / ml of vitamin C, 10-50 mg / ml of human albumin, 0.04-0.12 ml / ml of propylene glycol, 0.01-0.07 ml / ml of dimethyl sulfoxide and 0.5-3 mg / ml of lentinan. Compared with a cell cryo-preserved liquid in the prior art, the human albumin can replace fetal calf serum to achieve the effects of the fetal calf serum in the cell cryo-preserved liquid, and meanwhile, the cell cryo-preserved liquid is added with the non-essential amino acids, the vitamin C and the lentinan, thereby ensuring cell proliferation vitality after recovery is not influenced. The trehalose and the propylene glycol can replace a protective effect of dimethyl sulfoxide, thereby ensuring moisture in cells to be not crystallized during approach of freezing point. The components are cooperated with each other to achieve cryo-preservation of immune cells.

The present invention generally relates to devices and methods for the preservation of cells using drying, freezing, and other related techniques. In one set of embodiments, the invention allows for the preservation of cells in a dried state. In another set of embodiments, the invention allows for the preservation of cells within a glass or other non-viscous, non-frozen media. In some embodiments, the invention allows for the preservation of cells at temperatures below the freezing point of water, and in some cases at cryogenic temperatures, without inducing ice formation. The cells, in certain embodiments, may be preserved in the presence of intracellular and / or extracellular carbohydrates (which may be the same or different), for example, trehalose and sucrose. Carbohydrates may be transported intracellularly by any suitable technique, for example, using microinjection, or through non-microinjected methods such as through pore-forming proteins, electroporation, heat shock, etc. In certain instances, the glass transition temperature of the cells may be raised, e.g., by transporting a carbohydrate intracellularly. In some cases, the cells may be dried and / or stored, for example, in a substantially moisture-saturated environment or a desiccating environment. The cells may also be stored in a vacuum or a partial vacuum. The cells may be protected from oxygen, moisture, and / or light during storage. In certain cases, an inhibitor, such as a cell death inhibitor, a protease inhibitor, an apoptosis inhibitor, and / or an oxidative stress inhibitor may be used during preservation of the cells. The cells may be stored for any length of time, then recovered to a viable state, e.g., through rehydration, for further use.

The invention relates to a composite soil conditioner, comprising biogas residues, pyroligneous acid, mineral powder, soluble chitosan, diethylene triaminepentaacetic acid pentasodium, a surfactant, tobacco powder, trehalose, di-rhamnolipid, a flocculating component, a penetrating agent, enteromorpha powder, modified zeolite, boron-zinc-magnesium-containing fungicide, a nano additive, an accelerant, pentaerythritol triacrylate and ionic liquid, preferably also comprising 4-methyl phenyl valerate which is equal to the ionic liquid in weight. The novel composite soil conditioner has excellent soil improvement and recovery performance, the utilization rate of the fertilizer by the crop is greatly increased, and the composite soil conditioner can significantly increase the yield, simultaneously also can improve the permeability of the soil and avoids side effects of heavy metals such as cadmium; thus, the composite soil conditioner is beneficial to crop growth and has a wide industrial application prospect.

The invention discloses a cryoprotective agent of peripheral blood mononuclear cells. The cryoprotective agent is prepared from the following ingredients in parts by weight: 43-53 parts of a culture medium, 30-40 parts of auto-plasma, 12-17 parts of dimethyl sulfoxide, 5-7% of human serum albumin, and the balance being 0.17-0.23mol / L trehalose. The invention discloses a preservation method of the cryoprotective agent. The preservation method comprises the steps of collecting fresh anticoagulation blood, preparing peripheral mononuclear cells into cell suspension by using human serum albumin, adding the cryoprotective agent with the same volume as that of the cell suspension, mixing uniformly, preserving for 10-20h at below 90-below-70-below DEG C, and preserving in liquid nitrogen. According to the cryoprotective agent and the preservation method thereof, a cryopreserved cell has the high motility rate and the strong cell activity and can shorten the Cytokine Induced Killer (CIT) cell induced amplification period.