The invention relates to an MAAPN (Muscular Amino Acids and Peptides and Nucleosides) extractive containing
drug regimen ingredients such as polypeptide,
amino acid,
nucleoside and
nucleotide, as well as the composition thereof. The preparation method comprises the following steps: taking out heart and
muscle of a rabbit, removing
grease, homogenate, vacuum-pumping, leading in
carbon dioxide, then vacuum-pumping, and inflating repeatedly for two times; respectively adding
carbon dioxide saturated rabbit heart and
muscle slurry into
liquid carbon dioxide for stirring,
cooling down to 80 DEG C below zero, keeping for 6 to 10 hours, increasing temperature to 40 to 50 DEG C, respectively taking rabbit heart and
muscle after
freeze thawing according weight ratio of one to three and adding into injection water respectively, adding heart tissue solution into elastic
protease, and adding muscular tissue solution into papayotin; combining zymolytic heart tissue solution and muscular tissue solution, carrying out
centrifugation, taking supernatant fluid, adding 0.3 times of
activated charcoal in weight, stirring for 30 min in the
room temperature, and filtering through a
micropore filtration film.
Excipient can be added into the composition prepared by the extractive so as to make squirt,
powder for injection or freeze-dried
powder.