The invention provides a
filtration and microflow control device for circulating
tumor cells. The device comprises a negative-pressure sample feeding pump and a microflow control
chip, wherein the microflow control
chip comprises an upper-layer sample feeding layer, a plastic micro-hole filtering film and a lower-layer waste
liquid layer; a plurality of filtering holes are uniformly formed in theplastic micro-hole filtering film; the upper-layer sample feeding layer is communicated to a sample
test tube; and the lower-layer waste
liquid layer is connected to a negative-pressure feeding pump.The invention further provides a preparation process of the
filtration and microflow control device for the circulating
tumor cells. The preparation process comprises the following steps: preparing the upper-layer sample feeding layer and the lower-layer waste
liquid layer, preparing the plastic micro-hole filtering film, assembling the microflow control
chip and carrying out integral mounting. The invention further provides a working method of the
filtration and microflow control device. The working method comprises the steps of preparing a sample, capturing
tumor cells, adding an
antibody diluent and carrying out immunofluorescent
staining observation and counting. According to the filtration and microflow control device, the
cell capturing and
authentication functions are integrated, sothat the injury and
pollution of tumor cells in the transfer process are reduced; and the device has the characteristics of low cost, simplicity in operation and the like.