Filtration and microflow control device for circulating tumor cells as well as preparation process and working method of filtration and microflow control device

Abstract

The invention provides a filtration and microflow control device for circulating tumor cells. The device comprises a negative-pressure sample feeding pump and a microflow control chip, wherein the microflow control chip comprises an upper-layer sample feeding layer, a plastic micro-hole filtering film and a lower-layer waste liquid layer; a plurality of filtering holes are uniformly formed in theplastic micro-hole filtering film; the upper-layer sample feeding layer is communicated to a sample test tube; and the lower-layer waste liquid layer is connected to a negative-pressure feeding pump.The invention further provides a preparation process of the filtration and microflow control device for the circulating tumor cells. The preparation process comprises the following steps: preparing the upper-layer sample feeding layer and the lower-layer waste liquid layer, preparing the plastic micro-hole filtering film, assembling the microflow control chip and carrying out integral mounting. The invention further provides a working method of the filtration and microflow control device. The working method comprises the steps of preparing a sample, capturing tumor cells, adding an antibody diluent and carrying out immunofluorescent staining observation and counting. According to the filtration and microflow control device, the cell capturing and authentication functions are integrated, sothat the injury and pollution of tumor cells in the transfer process are reduced; and the device has the characteristics of low cost, simplicity in operation and the like.

Description

technical field [0001] The invention belongs to the technical field of circulating tumor cell identification, and more specifically relates to a microfluidic device for filtering circulating tumor cells, a preparation process and a working method thereof. Background technique [0002] Circulating tumor cells (CTCs) shed from the primary tumor into the peripheral circulation and further infect distant organs to form metastases. At present, many studies have proved that CTC is closely related to the development and metastasis of cancer. Therefore, by detecting the changes of circulating tumor cells in peripheral blood, the recurrence and metastasis of the tumor can be monitored in real time, and the progress of the tumor can be evaluated, which has important clinical value. [0003] Detecting CTCs is a challenge due to their rarity and heterogeneity. At present, there are mainly the following methods to capture CTCs: 1. Density gradient centrifugation: It is one of the class...

AI Technical Summary

Problems solved by technology

At present, there are mainly the following methods to capture CTCs: 1. Density gradient centrifugation: It is one of the classic methods for CTC capture, but the recovery rate of density gradient centrifugation is low and the degree of enrichment is not high, so the adoption rate is not high

2. Immunocapture and filtration methods are relatively recognized enrichment methods at present. The Cellsearch system certified by the U.S. FDA relies on EpCAM antibody for immunomagnetic bead sorting of CTCs. However, epithelial- Mesenchymal transformation (EMT), so some CTCs based on epithelial features cannot be detected by this method

Moreover, the high cost, complicated operation, and low throughput of this method limit its large-scale clinical application.

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