Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for simultaneously detecting content of six colorants in grape wine

A coloring agent and wine technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low recovery rate, easy loss in the process, low recovery rate, etc., and achieve high sensitivity, good precision, and good detection accuracy Effect

Inactive Publication Date: 2016-11-23
JIANGSU KINGS LUCK BREWERY
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the national standard GB / T 5009.35-2003, high performance liquid chromatography is recommended as the first method. The existing problems are: the sample pretreatment steps are cumbersome, the process is easy to lose, resulting in low recovery rate; the presence of bright blue in the sample will easily be absorbed by methanol- The formic acid mixed solution is washed away, and the recovery rate is low; the staff must be extremely skilled, and the detection efficiency of large batches of samples is low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for simultaneously detecting content of six colorants in grape wine
  • Method for simultaneously detecting content of six colorants in grape wine
  • Method for simultaneously detecting content of six colorants in grape wine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] The invention provides a method for simultaneously detecting the contents of six colorants in wine by liquid chromatography-mass spectrometry, and the six colorants include tartrazine, sunset yellow, amaranth, allura red, carmine and brilliant blue. The specific implementation of this method is as follows:

[0028] a. Sample pretreatment: Measure 25 mL of commercially available wine in a 50 mL beaker, preheat and boil until completely dry, add water to make up to 25 mL.

[0029] b. Preparation of colorant mixed standard solution: Weigh about 1g of colorant standard substance and dilute with ultrapure water to 100 mL respectively, as stock solution of mixed standard substance, tartrazine, allura red, amaranth, carmine , Brilliant Blue, and Sunset Yellow have a standard solution concentration of 4.527×10 -4 g / mL, 3.32×10 -4 g / mL, 9.282×10 -4 g / mL, 9.77×10 -4 g / mL, 9.28×10 -4 g / mL, 4.489×10 -4 g / mL; When using, draw a certain amount of stock solution and mix, then gr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for simultaneously detecting content of six colorants in grape wine. The method comprises the following steps that 1, a sample is prepared, wherein a certain volume of a wine sample to be detected is measured and boiled till the wine sample is dried to the maximum extent, ultrapure water is added for scale volume to the original volume, and the treated sample passes through a micropore filtration membrane; 2, a colorant mixed standard solution is prepared, wherein certain quantities of colorant standard substances are weighed separately, ultrapure water is added for scale volume to a certain volume, obtained colorant standard substance solutions are mixed in use, the mixed solution is progressively diluted to solutions with needed concentrations, and the diluted solutions pass through a water-phase micropore filtration membrane; 3, qualitative and quantitative analysis is conducted through a high performance liquid chromatography-quadrupole-electrostatic field orbitrap mass analysis combined instrument. The method is good in accuracy, high in sensitivity and good in precision when the method is used for detecting the six colorants in the grape wine sample, the recovery rate meets the analysis and detection requirements, and the method can be suitable for detection analysis to colorants in various grape wine.

Description

technical field [0001] The invention relates to the technical field of food analysis and detection, in particular to a method for simultaneously detecting the contents of six colorants in wine. Background technique [0002] Wine with health-preserving effects has become more and more popular in recent years, and the safety of wine has also attracted more and more public attention. [0003] The purpose of adding colorants to food is to improve the color of food, increase people's appetite, and increase its value. Food colorants can be divided into two categories: natural pigments and synthetic pigments. Synthetic pigments are widely used in the food processing industry due to their characteristics of low cost, diverse tones, bright colors and strong tinting strength. However, most of these colorants are azo compounds, which are generally synthesized from aromatic hydrocarbon compounds. Toxicological studies have found that synthetic pigments are chronically toxic or carcinog...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/02G01N30/06G01N30/14
CPCG01N30/88G01N30/02G01N30/06G01N30/14G01N2030/027G01N2030/062G01N2030/065G01N2030/146G01N2030/8809
Inventor 许桂林桂翔孙莹唐群勇季方董泉生孙丽香
Owner JIANGSU KINGS LUCK BREWERY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com