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A kind of preparation method of mussel oligosaccharide and its product and application

A technology of mussel polysaccharides and mussels, which is applied in the field of preparation of mussel oligosaccharides, can solve the problems of no reports of mussel oligosaccharides, and achieve the effects of mild conditions, simple process and high yield

Active Publication Date: 2020-08-18
SHANDONG ACADEMY OF PHARMACEUTICAL SCIENCES +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports about mussel oligosaccharides at home and abroad

Method used

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  • A kind of preparation method of mussel oligosaccharide and its product and application
  • A kind of preparation method of mussel oligosaccharide and its product and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 mussel polysaccharide preparation

[0038] (1) Raw material processing: take the dried frozen and boiled mussels with thick shells produced in Baolei County, Zhejiang Province as raw materials, pulverize them with a pulverizer, and pass through a 20-mesh sieve to obtain dried mussel powder;

[0039] (2) Polysaccharide extraction: put the dried mussel powder prepared in step (1) into the extraction tank, add 6 times the volume of water, extract at 100°C for 2 hours, and the rotation speed of the stirring paddle is 60rpm:

[0040] (3) Slag removal: Use a 50-mesh screen to separate mussel meat residue and polysaccharide extract:

[0041] (4) Enzymolysis: adjust the pH of the polysaccharide extract prepared in step (3) to 8.0 with NaOH, add alkaline protease with a mass ratio of 0.2%, and enzymolyze at 50°C for 2 hours, and after the enzymolysis is completed, place it at 80°C Inactivate for 15 minutes to obtain the enzymatic hydrolysis solution;

[0042] (5) ...

Embodiment 2

[0046] Embodiment 2 Yeast cell preparation

[0047] (1) Incline cultivation

[0048] Inoculate Saccharomyces cerevisiae (CICC No. 1002) on the slant of YPD medium (mass percentage: 2% peptone, 1% yeast powder, 2% glucose, 2% agar, natural pH), culture at 28°C for 48h, and rinse with sterile water Inclined surface to obtain a bacterial suspension, that is, a seed solution.

[0049] (2) shake flask culture

[0050] Put the seed solution into YPD medium (mass percentage: 2% peptone, 1% yeast powder, 2% glucose, natural pH), inoculum size is 5%, cultivate at 28°C for 30h, centrifuge at 10000rpm for 3min, discard the supernatant, and obtain Saccharomyces cerevisiae cells.

Embodiment 3

[0051] Embodiment 3 mussel oligosaccharide preparation

[0052](1) The mussel polysaccharide powder of Example 1 was dissolved in distilled water to prepare a 10% polysaccharide solution, and the pH was adjusted to 5.5 with HCl to obtain the mussel polysaccharide solution;

[0053] (2) adding medium-temperature α-amylase to the mussel polysaccharide solution described in step (1) to make the concentration 0.5 g / L, incubating at 50°C for 0.5h, and inactivating at 100°C for 15min to obtain a mussel polysaccharide hydrolyzate;

[0054] (3) The mussel polysaccharide hydrolyzate described in step (2) is filtered with a 0.22 μm filter membrane, and the filtrate is collected to obtain the mussel oligosaccharide solution I;

[0055] (4) Add the yeast cells of Example 1 to the mussel oligosaccharide solution I described in step (3) according to the mass volume ratio of 8%, culture on a shaking table at 30° C. and 150 rpm for 30 h, and centrifuge to get the supernatant to obtain mussels...

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Abstract

The invention provides a method for preparing Mytilus edulis oligosaccharides. The method comprises the following steps: dissolving Mytilus edulis polysaccharide powder in distilled water, adding alpha-amylase to carry out enzymolysis on principal chains of Mytilus edulis polysaccharides, adding saccharomycete cells into the enzymolysis solution to remove monosaccharides, disaccharides and trisaccharides, and carrying out activated-charcoal purification to remove undegraded polysaccharides, thereby obtaining the Mytilus edulis oligosaccharides with the polymerization degree of 4 to 8. The method for preparing the Mytilus edulis oligosaccharides, provided by the invention, has the characteristics of high yield, simple process, moderate conditions, easiness in amplification, and the like. Meanwhile, the invention further provides application of the Mytilus edulis oligosaccharides in preparation of blood fat regulating drugs or food. The Mytilus edulis oligosaccharides have a prospect for further research and development of blood fat lowering drugs and health-care food.

Description

technical field [0001] The invention relates to a method for preparing oligosaccharides, in particular to a method for preparing mussel oligosaccharides with a degree of polymerization of 4-8. Background technique [0002] Mussel (Mytilus sp.) belongs to a kind of bivalve shellfish and is an important species in marine shellfish farming. It is also known as sea red, commonly known as "egg in the sea". Due to the large output of mussels, it is not easy to preserve after harvesting, so they have always been cooked and processed into dry products - mussels. Mussels have high nutritional value and certain medicinal value, which are recorded in "Compendium of Materia Medica", "Supplements to Materia Medica" and "Rihuazi Materia Medica". Modern pharmacological studies have shown that mussels have the functions of improving immunity, anti-tumor, scavenging oxygen free radicals, anti-atherosclerosis, lowering blood lipids, inhibiting the formation of thrombus, improving myocardial ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/00A23L33/125A61K31/716A61P3/06C12R1/865
CPCA23L33/125A23V2200/00A61K31/716C12P19/00C12P19/14A23V2200/3262
Inventor 刘飞张金华朱希强张天娇邵华荣张林军袁超李珍爱侯重文凌沛学王凤山
Owner SHANDONG ACADEMY OF PHARMACEUTICAL SCIENCES
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