Complex microbial agent for producing soybean paste in Pixian County and preparation method thereof
A technology of compound microbial bacterial agent and watercress, which is applied in food preparation, application, food science, etc., can solve problems such as difficulty in popularization and application, instability, and uncontrollability, and achieve shortened production cycle, increased content, and increased amino nitrogen content Effect
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Embodiment 1
[0046] The processing steps of the present embodiment are as follows:
[0047] (1) Activation and expansion of strains
[0048] ●Strain activation
[0049] Put 10mL of normal saline with a mass concentration of 0.9% into 4 test tubes, sterilize at 121°C for 20 minutes, and then cool to 30°C. 1g each of Bacillus freeze-dried bacteria powder and halo-tolerant Tetradococcus freeze-dried bacteria powder were added to 4 test tubes under sterile conditions, shaken and dissolved, and placed in a 30°C constant temperature incubator to activate for 30 minutes for later use;
[0050] ●Expanded culture of bacteria
[0051] Insert the activated Lactobacillus plantarum and Halotolerance Tetradendococcus respectively into the bacterial expansion medium, and cultivate them on a shaker (130r / min) at 30°C for 21h; the volume percentage of the Lactobacillus plantarum bacterial solution is 1%, and the The volume percentage of said bacteria expansion medium is 99%; the volume percentage of sai...
Embodiment 2
[0070] The processing steps of the present embodiment are as follows:
[0071] (1) Activation and expansion of strains
[0072] ●Strain activation
[0073] Put 10mL of normal saline with a mass concentration of 0.9% into 4 test tubes, sterilize at 121°C for 20 minutes, and then cool to 30°C. 0.5g each of Bacillus freeze-dried bacteria powder and halo-tolerant Tetradococcus freeze-dried bacteria powder were added to 4 test tubes under sterile conditions, shaken and dissolved, and placed in a 30°C constant temperature incubator to activate for 40 minutes for later use;
[0074] ●Expanded culture of bacteria
[0075] Insert the activated Lactobacillus plantarum and tetradococcus halotolerant respectively into the bacterial expansion medium, and cultivate them in a shaker (180r / min) at 30°C for 24h; the volume percentage of the Lactobacillus plantarum bacterial solution is 2%, and the The volume percentage of said bacteria expansion medium is 98%; the volume percentage of said ...
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