The invention relates to a preparation method and application of an immobilized agent bag of an efficient denitrifier. The preparation method of the agent bag comprises the steps of screening out efficient heterotrophic
nitrification and
aerobic denitrification bacteria at first, and then carrying out salt-tolerant
domestication on the efficient heterotrophic
nitrification and
aerobic denitrification bacteria to obtain salt-tolerant heterotrophic
nitrification and
aerobic denitrification bacteria; adsorbing the immobilized salt-tolerant heterotrophic nitrification and aerobic
denitrification bacteria by using
modified zeolite to obtain immobilized salt-tolerant heterotrophic nitrification and aerobic
denitrification bacteria
zeolite; and then flatly laying the obtain immobilized salt-tolerant heterotrophic nitrification and aerobic
denitrification bacteria
zeolite in a cloth bag to obtain the final product. The bacterial density of
zeolite particles in the cloth bag is 1* 109-5* 109 CFU / mL. The prepared immobilized agent bag is widely used in
shallow sea aquatic farms and
aquaculture ponds, and efficient removal in a high-
salinity environment is realized; and moreover, the agent bag can be reused, the cost is greatly reduced, the shortcoming that free microbial
flora which are directly used easily flow along with tides of
seawater, cannot accurately position bacteria and cannotbe in full contact with the bacteria is overcome effectively, threatening to the quality of
aquaculture products due to introduction of alien microbes is also avoided,
ecological safety of water of aseawater
aquaculture region is protected, and the immobilized agent bag is expected to be widely used.