486 results about "Rice plants" patented technology

The invention provides specific transgenic rice plants, plant material and seeds, characterized in that these products harbor a specific transformation event at a specific location in the rice genome. Tools are also provided which allow rapid and unequivocal identification of the event in biological samples.

The present invention is in the field of plant biochemistry and genetics. More specifically the invention relates to nucleic acid molecules from plant cells, in particular, genomic DNA sequences from rice plants and nucleic acid molecules that contain markers, in particular, single nucleotide polymorphism (SNP) and repetitive element markers. In addition, the present invention provides nucleic acid molecules having regulatory elements or encoding proteins or fragments thereof. The invention also relates to proteins and fragments of proteins so encoded and antibodies capable of binding the proteins. The invention also relates to methods of using the nucleic acid molecules, markers, repetitive elements and fragments of repetitive elements, regulatory elements, proteins and fragments of proteins, and antibodies, for example for genome mapping, gene identification and analysis, plant breeding, preparation of constructs for use in plant gene expression, and transgenic plants.

Rice plants are disclosed with two separate, but synergistic mechanisms for resistance to herbicides that normally inhibit a plant's acetohydroxyacid synthase (AHAS) enzyme. The herbicide resistance of plants with both resistance mechanisms is substantially greater than one would expect from a simple combination of the two types of resistance. The first of the two resistance mechanisms is a metabolic pathway that is not fully understood, but that does not itself involve a mutant AHAS enzyme. The second resistance mechanism is a mutant AHAS enzyme, an enzyme that shows direct resistance to levels of herbicide that normally inhibit the enzyme, in both in vivo and in vitro assays. Besides controlling red rice, many AHAS-inhibiting herbicides also effectively control other weeds that are common in rice fields. Several of these herbicides have residual activity, so that a treatment controls both existing weeds as well as weeds that sprout later. No herbicide currently available for use on rice has residual activity against a broad spectrum of weeds including red rice. With effective residual activity against red rice and other weeds, rice producers now have a weed control system superior to those currently used.

The present invention provides a rice plant that shows enhanced growth and increased seed production, which also enables reduction of the use of chemical fertilizers.In the present invention, a nitrogen-fixing endophytic bacterium is isolated from bacteria symbiotically inhabiting natural plants, the isolated endophytic bacterium is artificially proliferated and then artificially inoculated into rice plants, and thus, the nitrogen-fixing endophytic bacteria are allowed to infect to rice plants.

The invention discloses application for using an LbCpf1 - RR mutant in a CRISPR / Cpf1 system in plant gene editing. The application for using the LbCpf1 - RR mutant in the CRISPR / Cpf1 system in plant gene editing uses an OsPDS gene and an OsSBEIIb gene as target genes, constructs a target double loci of one gene and a series of vectors of two genes, and utilizes an agrobacterium-mediated transformation method to import the vectors into rice callus, and rice plants with the target gene knockout are successfully obtained by using the LbCpf1-RR mutant. The only difference between the LbCpf1-RR mutant and the protein LbCpf1 is that the 532nd amino acid changes from G to R, and the 595th amino acid changes from K to R. The LbCpf1-RR mutant provided by the application for using the LbCpf1 - RR mutant in the CRISPR / Cpf1 system in plant gene editing expands the PAM site sequence identified by the LbCpf1-RR mutant, so that the editing range of the CRISPR / Cpf1 system in rice genome is expanded, great significance for promoting the application of the system in the field of plant genome editing is achieved. The application for using the LbCpf1 - RR mutant in the CRISPR / Cpf1 system in plant geneediting has great application value.

The invention discloses application of an OsKTN80b gene in terms of reducing rice plant height, belonging to the field of rice gene engineering. A plant with reduced plant height is finally obtained mainly by performing gene editing on the OsKTN80b gene through a CRISPR / Cas9 system, so as to obtain a transgenic plant subjected to OsKTN80b gene knockout, and selecting a homozygous mutant. The invention further discloses two target spot sequences SG1 or SG2 used for the OsKTN80b gene knockout; the target spot sequences are respectively formed by nucleotide sequences shown as SEQ ID No. 4 and SEQ ID No. 5. Compared with wild type rice, the plant height of the transgenic plant subjected to the OsKTN80b gene knockout is reduced by 13.56 to 16.33 percent, and the effect of reducing the rice plant height is good; secondly, a method provided by the invention is not limited to a genetic background, and the problems linked with mal-characters do not exist.

The invention discloses ultra-low-capacity liquid containing dinotefuran, which takes the dinotefuran or a combination of the dinotefuran and an active component II as an active ingredient. Other components comprise assistants and solvents which are complemented to form ultra-low-capacity liquid with content being 100 percent. The active component II is any one of imidaclothiz, high-efficacy cypermethrin, cypermethrin, ethofenprox, tolfenpyrad, flonicamid, chlorantraniliprole and the like. The ultra-low-capacity liquid containing the dinotefuran can be used for preventing and controlling cropinjurious insects such as rice plant hoppers, rice leaf folders, brown plant hoppers, white back plant hoppers, small brown plant hoppers, rice stem borers, rice thrips and the like, and has the advantages of convenience in production, water saving, high working efficiency, good efficacy, long efficacy duration, synergistic interaction and the like.

The present invention belongs to the field of agricultural cultivation technology, and particularly relates to a high-yield cultivation method of organic selenium-enriched rice. The method includes the following steps: A, selecting rice seeds and sterilizing the rice seeds; B, arranging rice seedling beds; C, sowing the rice seeds and cultivating rice seedlings; D, preparing a large field; E, transplanting the rice seedlings; F, managing the field; and J, harvesting the rice. Insect disinfestation for the rice seeds is carried out using Tripterygium wilfordii extracts; meanwhile, Tripterygium wilfordii extracts are used to dissolve the selenium and biological pesticides in the rice production process, and further improvements on the prevention and control of plant diseases and insect pests are realized in the overall growth process of the organic selenium-enriched rice. Instead of chemical fertilizers, bio-organic fertilizers are used in the rice cultivating process, which ensures a variety of nutrients required in the rice cultivating process and improves the rice yields. The rice yields and the selenium content of the organic selenium-enriched rice are higher than those of rice planted with traditional cultivation technology, and pesticide residues are significantly lower than that of the rice planted with the traditional cultivation technology.

The invention discloses an anti-rice blast gene Pi9 specific CAPS marker Pi9caps and application thereof. The invention discloses a method for identifying or auxiliarily identifying whether rice contains anti-rice blast gene Pi9 and / or whether rice has rice blast resistance, which comprises the following steps: detecting 40804th nucleotide from the 5' terminal of 75-1-127BAC12.1 sequence or homologous sequence thereof in chromosome 6 of rice to be detected, and identifying according to the kind of the 40804th nucleotide the 5' terminal of the 75-1-127BAC12.1 sequence or homologous sequence thereof in chromosome 6 of rice. The anti-rice blast gene Pi9 specific CAPS marker Pi9caps can accurately judge whether the selected rice plant contains the anti-rice blast gene Pi9, and thus, has important application meanings in accelerating the anti-rice blast rice species breeding progress.

The present invention relates a method for transforming a rice plant to provide it with a C4 photosynthetic pathway by way of the introduction of some genes participating in a C4 photosynthetic pathway. To this end, the method of the invention comprises introducing a phosphoenolpyruvate carboxylase (PEPC) and a gene coding for a phosphoenolpyruvate carboxykinase (PCK) which has been connected with a DNA fragment coding for a transit peptide into a rice plant.

The invention discloses an application process of a rice plant growth regulator. The rice plant growth regulator consists of a rice plant growth regulator in a rice seedling stage, a rice plant growth regulator in a tillering stage, and a rice plant growth regulator in a reproductive stage, wherein the rice plant regulator in the rice seedling stage is used for the rice seedling stage, the rice plant growth regulator in the tillering stage is used for the tillering stage, and the rice plant growth regulator in the reproductive stage is used for the reproductive stage.

A rice cultivar designated CL171-AR is disclosed. The invention relates to the seeds of rice cultivar CL171-AR, to the plants of rice CL171-AR, to methods for producing a rice plant produced by crossing the cultivar CL171-AR with itself or another rice variety, and to methods for controlling weeds in the vicinity of plants of rice cultivar CL171-AR, which comprises increased resistance to acetohydroxyacid synthase-inhibiting herbicides. The invention further relates to hybrid rice seeds and plants produced by crossing the cultivar CL171-AR with another rice cultivar.

The invention is directed to expression of non-plant proteins in rice plants. Expression is optimized by use of a non-rice promoter of a monocot protein gene and its corresponding signal peptide for expression of the non-plant protein in rice plant at high yields. The invention is useful for making human proteins, polypeptides and peptides in rice seeds. The expressed protein product can be isolated from the rice seed for administration to humans or other animals.

The invention discloses a protein relative to rice tillering as well as a coding gene and the application thereof. The protein relative to rice tillering is the protein with one of the following amino-acid residue sequences: (1) SEQ ID NO: 3 in a sequence list; (2) the protein, in which the SEQ ID NO: 3 in the sequence list is replaced, deleted or added by one or a plurality of amino-acid residues, and which is correlated with the rice tillering. The protein relative to rice tillering and the coding gene thereof play an important role in the reasonable configuration of the rice plant structure and the further rice yield improvement.

The invention relates to the field of plant breeding, and particularly relates to the fertility restoration of a homozygous recessive genic male sterility rice plant. Furthermore, the invention relates to a method for establishing a plant male sterile line and a maintainer line and a genetic transformation material, and more particularly relates to a structure, a rice cell, tissue or organ, a method for establishing a rice male sterile line and a maintainer line, a method for restoring the male fertility of a rice sterile plant, a method for preparing rice seeds, a genetic transformation material, a method for preparing hybrid rice and an application of the rice male sterile line in preparation of hybrid rice.

It is intended to establish a technology of controlling Gramineous plant seed-borne disease that avoids the danger of development of resistant pathogeus, being highly safe to environment andstable. There is provided a fungus having activity of controlling fungal disease and bacterial disease occurring during the raising of rice plant seedling, and are further provided, using the fungus as an active ingredient, a controlling agent, method of controlling and biological material.

A rice planting method for obtaining rice planted in a field with frogs belongs to the technical field of the stereo ecological effective production combining scientific planting and culturing. The rice planting method is as follows: frogs are cultivated while rice is planted in the rice field; specific measures are as follows: a waterhole is arranged at a water inlet of the rice field; a ditch isarranged along the periphery of ridge of the rice field; a plastic thin film with 1.2m height surrounds a defensive wall which preventing the frogs from escaping on the periphery of ridge of the ricefield; mound for the wintering of the frogs is arranged in the rice field; after rice is planted in the rice field, the water depth needs to be kept over 3cm; about five thousands of frogs are cultivated per acre. Growing environment of the rice is used for cultivating the frogs which prey on pests and other insects growing in the rice field; excrement of the frogs is used as fertilizer of rice growing. Water body resources and ecology environment of the rice field are fully utilized by cultivating the frogs in the rice field, thus being capable of achieving effects of rice and frog intergrowth and mutual benefit, efficiency improvement and energy consumption. The rice planted in the field with the frogs is pure green superior rice with high grade; and the frogs have very high economicalvalue.

The invention belongs to the field of genetic engineering of plants and discloses a method for building a high-efficiency regenerating and transforming system of Oryza sativaL. subsp. japonica 11 through cultivation of induction of embryogenic callus, subculture multiplication of embryogenic callus, transformation mediated by agrobacterium tumefaciens, cocultivation of embryogenic callus and agrobacterium tumefaciens, screening and cultivation of resistant callus and seedlings differentiation of resistant callus. The method has the advantages of simple technological processes, low production cost, and capabilities of obviously increasing the inductivity of the embryogenic callus, the subculture multiplication rate and the differentiation and regeneration rate of the embryogenic callus of the Oryza sativaL. subsp. japonica 11 and simultaneously shortening the period of obtaining regenerative rice plants; and on the basis, an improved agrobacterium tumefaciens-mediated method can be used for remarkably increasing the efficiency of obtaining the transgenosis rice plants and shortening the period of obtaining regenerative rice plants.

The invention belongs to the field of genetics and breeding, in particular relates to a rice plant type related gene RL10 and application thereof. The rice gene RL10 has a sequence as shown in SEQ ID NO.1, and the rice gene RL10 codes F-box proteins and controls the polar growth of rice leaves and the morphogenesis of rice panicle traits. The gene can improve the plant types and the panicle types of the prior species, improve the yield, and has wide application prospect in the high-yield breeding of rice.

The invention discloses a cultivation method for obtaining rice plants, which simultaneously show male-sterile and dwarf properties, through genetic engineering. Dwarf male-sterile rice with simultaneous incomplete expression of male-fertile genes and plant height genes is cultivated with an RNAi (Ribonucleic Acid interfere) technology by using a GA20-oxidase gene (OsGA20o*2), which has an effect of stretching rice stems and is used for gibberellin synthesis, and an RTS (Ricetapetum-Specific) gene which plays a key role in development of rice anther tapetum; the male-sterile and dwarf properties of the dwarf male-sterile rice simultaneously occur; and the rice can be normally fertilized to bear fruits after receiving wild type plant pollen. The dwarf male-sterile rice can be widely applied to rice recurrent selection breeding and has great significances to rice genetic breeding and production.

The invention relates to a rice-fish comprehensive planting and breeding technical method. The method is a production method of cultivating and planting ratoon rice after harvesting of single-season rice and continuing fish stocking in the rice field by the utilization of the ecological principle of rice fields. According the ratoon rice, after ripening of the single-season rice, about upper 2 / 3 parts of rice plants are left, ears of rice are picked and the lower 1 / 3 part of the plants and roots is left; after fertilization and cultivation, the second season rice grows out. Breeding of fish inthe rice field runs through the whole process. Melons and vegetables are planted in the ridge, thus creating the three-dimensional circulating rice-fish comprehensive planting and breeding model of ''single-season rice+ ratoon rice + fish'', namely the ''single-season rice+ ratoon rice + fish'' model. The technical method of the invention can be promoted and applied in rice fields in mountainousareas; the bred Sanjiang rice field carp contains rich selenium, which is very beneficial to the human body; and good economic, social and ecological benefits can be obtained.

This invention pertains to plants, plant material and seeds characterized by harboring a specific transformation event particularly by the presence of the bar gene under control of a CaMV 35S promoter, at a specific location in the rice genome. The rice plants of the invention combine glufosinate tolerance with optimal overall agronomic performance, genetic stability and adaptability to different genetic backgrounds.

The invention discloses a method for improving inoculation induction rate of rice blast, which belongs to the technical field of agricultural plant protection. The method comprises the following steps: firstly, collecting conidiospores of rice blast fungi; secondly, preparing inoculation liquid of rice blast fungi; thirdly, spraying the inoculation liquid for inoculation; and fourthly, observing, recording, counting and the like. In the invention, water retention agent and surface-active agent are added into the inoculation liquid, so that water in the inoculation liquid can be kept effectively; moreover, the hydrophilcity and the spreading property of the surface of a rice plant are improved, so that not only is the amount that the rice plant accepts the inoculation liquid improved, but also the time that the inoculation liquid stays on the rice acceptor is prolonged, further, favorable conditions are provided for induction of rice blast fungi, and the induction rate of artificial inoculation of rice blast is improved remarkably. The method can be popularized and applied to the study of rice blast as well as resistance identification and resistance breeding of rice blast.

The present invention provides a molecule marking method for a rice anti-rice blast gene Pigm, wherein four special molecule markers G1408, G2997, G2723 and G5301 are screened. According to the present invention, the four special molecule having co-segregation with the anti-rice blast gene are adopted to carry out PCR amplification and agarose gel electrophoresis detection on genome DNA of a rice parent strain or a hybridization generation, and the rice plant containing any one of the four special molecule markers after detection can be adopted as a breeding material containing high anti-rice blast Pigm gene. The method has characteristics of simple operation and accurate detection result, and can meet requirements of high-throughput molecule marker-assisted breeding.