This invention relates to
plant breeding and the protection of plants from insects. More specifically, this invention includes novel transformation events of cotton plants comprising one or more
polynucleotide sequences, as described herein, inserted into specific site(s) within the
genome of a cotton
cell. In highly preferred embodiments, said
polynucleotide sequences
encode “stacked” Cry1F and Cry1Ac lepidopteran
insect inhibitory proteins. However, the subject invention includes plants having single cry1F or cry1Ac events, as described herein. Additionally, the invention is related to cotton plants derived from that transformation event and to assays for detecting the presence of the event in a sample. More specifically, the present invention provides
DNA and related assays for detecting the presence of certain
insect-resistance events in cotton. The assays are based on the
DNA sequences of recombinant constructs inserted into the cotton
genome and of the genomic sequences flanking the
insertion sites. These sequences are unique. Based on these insert and border sequences, event-
specific primers were generated.
PCR analysis demonstrated that these cotton lines can be identified in different cotton genotypes by analysis of the PCR amplicons generated with these event-specific primer sets. Thus, these and other related procedures can be used to uniquely identify these cotton lines. Kits and conditions useful in conducting the assays are also provided. These materials and methods can also be used to assist breeding programs to further develop traits in cotton.