The invention discloses an ecological breeding method of efficient polyculture of pseudorasbora parva, crab larva and loach. The method comprises the steps of: pond conditions, disinfection, breeding water preparation, grass planting, fry breeding, water level adjustment, water quality adjustment and control, feeding management, disease control, daily management and the like. The breeding method provided by the invention focus on the breeding of crab larvae; the pseudorasbora parva is mainly used as cleaners for residual feeds, so that the pollution to water quality can be greatly reduced, the water quality can be improved, the growth of crab larvae and loaches is promoted, and meanwhile, blue-green algae outbreak in the crab larva pond can be biologically controlled by the polyculture of the pseudorasbora parva; the loaches play a role in loosening the soil, and eliminates hazardous substances in the soil in time, so as to avoid the water quality becoming bad due to the high temperature in summer, in addition, loaches do not need additional feeding and management, are beneficial supplement to the breeding of the crab larvae, and thus the pond benefit is greatly increased.