The present invention discloses a feed for procambarus clarkii and a preparation method thereof. The feed for procambarus clarkia comprises the following raw material components: flour, rice bran, rapeseed meal, peanut meal, soybean meal, super steam fish meal, cottonseed meal, corn gluten meal, bentonite, brewer's yeasts, spray-dried animal blood cells, squid cream, monocalcium phosphate, semen ricini kernels, phosphatide oil, fish oil, choline, a mildew preventive, shrimp trace elements, shrimp vitamins, vitamin C, an antioxidant, inositol and carnitine. The feed for the procambarus clarkii can inhibit the gonad development and inhibit natural ovulation in the development period of the procambarus clarkia, thereby increase the weight gain, and increase the economic benefits. The preparation method of the feed for the procambarus clarkii is simple and convenient in operation, and conductive to improving the breeding efficiency.