The invention discloses paeonia suffruticosa vinegar which contains active ingredients extracted from paeonia suffruticosa, wherein the active ingredients comprise paeonin, paeonol, astragalin, flavonoid, organic acid, amino acid, mineral substances, vitamins and other nutrition ingredients. The paeonia suffruticosa vinegar is mild in tart taste, slightly sweet, pleasant in fragrance and rich in nutrition, can be directly drunk, and is taken as a natural green nutritious health-care product. The paeonia suffruticosa vinegar has the functions of reducing blood fat, softening blood vessels, reducing blood pressure, removing free radicals such as O-2 and.OH in human bodies, resisting oxidation and ageing, adjusting immunity, moisturizing skin and nourishing face as well as helping digestion and dispelling the effects of alcohol. The invention also discloses a preparation method of the paeonia suffruticos vinegar.