238results about How to "Clear composition" patented technology

The invention relates to the culture medium research and development technical field of modern biological technology and provides a serum-free medium for MDCK cell large-scale adherent culture and single-cell suspension culture, which comprises 21 amino acids, 6 vitamins, 8 salts, 8 lipids, 4 trace elements, 2 buffers, 1 protein hydrolysate, 1 acid-base indicator and 6 other additives. The serum-free medium can be prepared by the conventional preparation method, and an application method thereof is the conventional method. The serum-free medium has the beneficial effects that: the serum-free medium does not contain serum, has clear components, is beneficial for separating and purifying the product and improves the product quality; the serum-free medium supports long-term subculture of MDCK cells and does not require long-term and complex adaptation process; and the serum-free medium can well support the adherent growth and single-cell suspension growth of the MDCK cells, has clear components and easy preparation and utilization, and is suitable for mass production of biological products.

The invention relates to a leaven dedicated for fermenting bed of microorganism engineering constituting in weight ratio: Lactobacillus of 15%-25%, enterococcus of 15-30%, yeast of 5-15%, bacillus subtilis of 10-30%, Bacillus licheniformis of 5-20%, Lactobacillus sporogenes of 5-20%; total live microbe being 5-10 hundred million/g. The leaven of the invention is composed of biotic bacteria with strong activity, and has definite composition and mechanism, and stable product quality, and good health effect for improving immunity of the animal and preventing disease, strong degradation ability for pig manure; the leaven can not degrade material of fermenting bed pad, can prolongs life of fermenting bed; the fermenting bed can be conveniently and simply manufactured, which saves more than 80% of labor.

An aqueous, heat and cold stable, non-alcoholic or slightly-alcoholic microemulsion based antimicrobial mouthwash composition with improved antimicrobial efficacy. The composition comprises a unique water-soluble matrix composite, at least one water-immiscible or water-insoluble antimicrobial agent, and optionally, a preservative or preservative system, a weak carboxylic acid, a coloring agent and other additives. Examples of antimicrobial agents include menthol, thymol, eucalyptol and/or methyl salicylate. A process for preparing the oral care composition is also disclosed.

A bamboo root amino-acid peptide extract, its production and use are disclosed. The extract contains amino acid 10-50wt%, which consists of free amino acid 5-40wt%, tyrosine, serine, aspartine, glutamic acid, alanine, phenylalaine, valine and delta-OH-Lys. It has good taste and can be used for functional food, beverage and condiment.

The invention discloses an osmanthus phenylethanoid glycoside extract. The total phenylethanoid glycoside content in the osmanthus phenylethanoid glycoside extract is 30-90 wt%; and the total phenylethanoid glycosides comprise verbascoside and isoverbascoside. The verbascoside content in the osmanthus phenylethanoid glycoside extract is 25-80 wt%, and the weight ratio of the verbascoside to the isoverbascoside in the total phenylethanoid glycosides is 48:1-300:1. The invention also discloses a preparation method of the osmanthus phenylethanoid glycoside extract and application of the osmanthus phenylethanoid glycoside extract in preparing foods, drugs or health products for preventing and treating diabetes.

The invention discloses a method for quickly determining anthocyanin in a seed coat of a black peanut by using UPLC/MS/MS (Ultra Performance Liquid Chromatography/tandem Mass Spectrometry), belonging to the technical field of detection. The method comprises the following steps of (1) extracting anthocyanin in the seed coat of the black peanut, (2) purifying anthocyanin in the seed coat of the black peanut, and (3) analyzing and determining anthocyanin in the seed coat of the black peanut. With the adoption of the method, anthocyanin in the black peanut can be extracted quickly and fully, so that the kind and the content of different anthocyanin can be determined precisely; the method has an important significance in revealing a material basis and an action mechanism of an activity function of anthocyanin; and a theoretical foundation is provided for developing the peanut variety rich in anthocyanin content and improving the nutritional quality and a health-care function of the peanut.

The invention provides a method for rapidly separating and extracting hUC-MSC (human Umbilical Cord mesenchymal stem cells). The method comprises the following steps: taking the freshly collected umbilical cord tissue of a healthy newborn baby, carrying out on-ice transportation on the freshly collected umbilical cord tissue in umbilical cord storage transportation liquid containing double antibodies, carrying out cleaning and disinfection by adopting 75% alcohol and normal saline, removing blood vessels, carrying out blunt dissection on wharton jelly, carrying out mechanical pulverization, treating the obtained product I by adopting red blood cell lysis buffer for 3 min, digesting the obtained product II by adopting IV collagenase, screening the obtained product III by adopting a 100-200-mesh sieve, carrying out suspension culture on the obtained product IV by adopting a serum-free medium, wherein the liquid is changed every 3-5 days, taking supernatant, detecting cell pollution, after the adherent rate in a plate reaches 30-70%, carrying out trypsinization, carrying out centrifugation, collecting cells, carrying out passage amplification, carrying out merging when the cell merging rate reaches 90% or above, collecting the cells, carrying out cryopreservation on the cells, and detecting the biological characteristics of hUC-MSC.

The invention relates to a Chinese traditional medicine active extract, a preparation method thereof and an application thereof, particularly relates to an effective part extracted from a Chinese traditional medicine Qinglongyi, its preparation method and its application. The Qinglongyi active extract is prepared by the following steps of ethanol diacolation, reflux, ultrasonic extraction, CO2 supercritical extraction, ethanol precipitation, elution, interception, reflux, concentration, dryness and the like. According to the invention, the anticancer activity of the Qinglongyi effective part is taken as an index for screening the effective part of Qinglongyi, efficacy tests are carried out by using the extract with different contents, the result proves that an effective component in the Qinglongyi active extract for treating cancer is total naphthoquinone; the efficacy test result proves that an effective component for anti-inflammatory analgesic effect is total flavonoid, total tannin and polar micromolecules; a substrate for improving immune function is polysaccharide.

The invention relates to a serum-free culture medium for amplification in vitro of a human immune killer cell, wherein the serum-free culture medium is formed by combining and mixing a basal culture medium and a serum replacement, and the serum-free culture medium comprises the basic components of 2 saccharides, 22 amino acids, 6 lipids, 2 nucleosides, 11 vitamins, 16 salts, 1 hormone, 2 protides, hydroxyethylpiperazine ethane sulfonic acid and phenol red. The serum replacement is combined and added into the basal culture medium to prepare the serum-free culture medium. The components of the serum-free culture medium are definite, and the serum-free culture medium supports amplification in vitro of the immune killer cell, achieves high efficiency and stability, promotes the further study and application of biological therapy, promotes the more smooth progressing of the immune cell therapy, and benefits the nation and the people.

The invention discloses monosubstituted metal phthalocyanine and a synthesis and separation method and application thereof. The conventional synthesis and separation of a metal phthalocyanine coordination compound are limited by experimental conditions and are not suitable to be widely applied. The monosubstituted metal phthalocyanine is prepared by the following steps of: reacting substituted phthalonitrile 1 with substituted phthalonitrile 3 in the presence of n-amyl alcohol, DMF (N,N-dimetbylformamide) or DMSO (dimethyl sulphoxide) according to a molar ratio; adding metal salt and organic base, wherein the molar ratio of the metal salt to the substituted phthalonitrile 1 is 1:1-1:5, the molar ratio of the organic base to the substituted phthalonitrile 1 is 3:1-10:1, the molar ratio of the n-amyl alcohol, the DMF or the DMSO to the substituted phthalonitrile is 10:1-20:1 and the reaction is performed at the temperature of between 120 and 200 DEG C for 15 to 30 hours to generate a monosubstituted metal phthalocyanine-containing mixture; and separating the mixture by a dry-column chromatography. The method is used for preparing new monosubstituted metal phthalocyanine.

The invention belongs to the field of immunodetection and provides a method for diagnosing bovine tuberculosis mediated by a recombinant protein mixture and a regent for diagnosing bovine tuberculosis mediated by the recombinant protein mixture; the reagent comprises the recombinant protein mixture used as specific irritant; animals infected by mycobacterium bovis can be simulated by the recombinant protein mixture so as to generate a DTH (delayed type hypersensitivity) reaction; compared with a PPD (purified protein derivative) intradermal allergic reaction test, a intradermal allergic reaction test established by regent provided by the invention has higher specificity; mycobacterium bovis infection can be distinguished from environmental mycobacteria infection; and therefore, the method and the reagent can be effectively used for clinical detection of the bovine tuberculosis.

The invention provides an organic rare earth complex NH4[Eu(C7H6O3)4(phen)2] and a preparation method thereof and application of the organic rare earth complex serving as a photoluminescent material. According to the organic rare earth complex, a europium ion is adopted as a coordination center atom, salicylic acid and 1,10-phenanthroline serve as organic ligands, lowest triplet energy levels of the organic ligands can be well matched with the resonance emission energy level of the europium ion, the organic rare earth complex has a good light absorption property in the ultraviolet region and has a high fluorescence quantum yield under the excitation of ultraviolet light, and therefore the organic rare earth complex has a good light conversion property; it is shown through X-ray diffraction testing results that the component and structure of the organic rare earth complex are definite; the organic rare earth complex NH4[Eu(C7H6O3)4(phen)2] can adsorb the ultraviolet light with the wavelength ranging from 200 nm to 400 nm and converts the ultraviolet light into visible light with the wavelength of 618 nm.

The invention is suitable for the field of medicines and provides a drug composition for preventing and treating related diseases of ischemic stroke. The drug composition comprises the following components in parts: 1.0-3.5 parts of notoginsenoside R1, 4.0-7.5 parts of ginsenoside Rg1, 3.5-7.5 parts of ginsenoside Rb1, 1.0-3.0 parts of ginsenoside Re, 0.03-0.15 part of astragaloside, 0.02-0.010 part of calycosin-7-O-beta-D-glucopyranoside, 0.01-0.10 part of harpagoside, 0.002-0.010 part of cinnamic acid, 0.45-1.5 parts of salvianolic acid B, 0.02-0.10 part of cryptotanshinone and 0.02-0.10 part of tanshinone IIA. The drug composition provided by the invention has the functions of promoting blood circulation and removing blood stasis, supplementing qi and nourishing yin, can be used for preventing and treating the related diseases of the ischemic stroke and is capable of overcoming the shortcomings of component complexity and the like of traditional compound traditional Chinese medicine preparations.

The invention discloses a method for preparing collagen peptide by hydrolyzing bovine bones, and the collagen peptide. The method comprises the following steps: S1, cleaning bovine bones, and freezingand preserving the cleaned bovine bones at a temperature from -20 DEG C to -1 DEG C for 3-11 hours; S2, grinding and crushing the frozen and preserved bovine bones in step S1 into bone residues; S3,adding 0.5-1.5 times of water into the bone residues in step S2, performing homogenizing, adjusting the temperature to 40-50 DEG C, then adding protease, and performing enzymolysis under a certain condition; and S4, performing enzyme deactivation treatment after the enzymolysis is finished, performing filtering by a plate-frame filter press or performing purifying refining by membrane separation,and performing drying after double-effect concentration to obtain the bovine bone collagen peptide. The technological method provided by the invention has the advantages of short enzymolysis time andgood enzymolysis effect; and the obtained protein peptide product is easy to dissolve in water, and has a high nutritional value and good dispersibility.