315 results about "Salvianolic acid" patented technology

The invention discloses an extraction method of salvianolic acid B and hydroxy safflor yellow A as well as a preparation method of danhong for injection, and relates to the extraction method of the salvianolic acid and the hydroxy safflor yellow as well as the preparation method of the danhong preparation. The invention solves the problems that the extraction of the salvianolic acid B and the hydroxy safflor yellow A is not thorough, and the transportation and the storage of the prepared danhong injection are inconvenient. The salvianolic acid B of the invention is extracted according to the following steps: backflow, acidification and adsorption; thus the salvianolic acid B is obtained. The hydroxy safflor yellow A of the invention is extracted according to the following steps: boil out, acidification and adsorption; thus the hydroxy safflor yellow A is obtained. The danhong for injection is extracted according to the following steps: compounding ratio and standing, filtrating and lyophylization; thus the danhong for injection of the invention is obtained. The extraction rate of the salvianolic acid B and the hydroxy safflor yellow A of the invention is high, and the transportation and the storage of the prepared danhong injection are convenient.

The invention discloses a method for manufacturing salvianolic acid A of danshen root, which is characterized in that total salvianolic acid in the danshen root is transformed into salvianolic acid A by adopting a certain method. The method increases greatly the extraction yield of salvianolic acid A. The salvianolic acid A is prepared into tablets, capsule, granula, soft capsule, micro pill, dripping pill and oral liquid, which have good pharmacological action indicated by pharmacological experiments.

The invention relates to a method for increasing the contents of tanshinone and salvianolic acid in a salvia miltiorrhiza hairy root by using a transgene AtMYC2, belonging to the technical field of gene engineering. The method comprises the steps of constructing a high-efficiency expression vector of a plant by using an arabidopsis transcription factor AtMYC2, and carrying out genetic transformation on salvia miltiorrhiza leaves to obtain a gene AtMYC2 overexpressed transgenetic salvia miltiorrhiza hairy root; analyzing the expression of AtMYC2 in the transgenetic salvia miltiorrhiza hairy root and related genes in biosynthetic pathways of tanshinone and salvianolic acid through qRT-PCR; measuring the contents of tanshinone and salvianolic acid in the transgenetic salvia miltiorrhiza hairy root by using a high-performance liquid chromatography (HPLC); and measuring the antioxidant activity of tanshinone and salvianolic acid in the transgenetic salvia miltiorrhiza hairy root by using a DPPH free radical scavenging method. The invention provides the method for simultaneously increasing the contents of tanshinone and salvianolic acid in salvia miltiorrhiza hairy root and also provides a novel high-quality raw material for producing tanshinone and salvianolic acid with important clinic demands so as to have the positive promoting significance and application value for relieving the problem that the drug resources of tanshinone and salvianolic acid are short.

Compositions are provided containing molecules having at least one moiety from beta-(3,4-dihydroxyphenyl)lactic acid and/or caffeic acid, which are found in extracts from the plant genus Salvia, said moieties being of formula(I), the active agents having a molecular weight of at least 190 daltons. A class of preferred agents are those which are conjugated to form dimers, trimers, tetramers and larger polymers containing said moieties, with the most preferred being salvianolic acid dimers, trimers, tetramers and larger polymers. The compounds and compositions may be administered in pharmaceutically acceptable carriers and excipients systematically or locally to treat viral infections.

The invention relates to establishment of a salvianolic acid extract fingerprint spectrum and a content measurement method of related components. The content measurement method includes the following steps: 1) establishing the salvianolic acid extract digitized quantitative fingerprint spectrum through ultrahigh performance liquid multi-wavelength visible ultraviolet detection method; 2) recognizing peaks in the fingerprint spectrum in the step 1) with mass spectrum to determine common fingerprint peaks of ultraviolet detection; and 3) performing measurement to contents of three indicative components, which are high in content, significant in activity and definite in structure, recognized in the step 2) through high performance liquid chromatography variable-wavelength detection method.

The present invention relates to one kind of phenolic acid compound and its preparation process and use in treating AIDS. Red sage material is treated through solvent extraction, macroporous adsorbing resin purification, conventional drying and other steps to obtain total phenolic acid. The total phenolic acid is dissolved in water via heating, chromatographic column separation and repeated purification to obtain new compound salvianolic acid N. Enzyme and cell activity experiments show that the new compound has HIV resisting activity and is hopeful in being developed into one new kind of AIDS treating medicine.

The invention discloses a preparation method of a high-content salvianolic acid B. The method comprises the following two steps: separating total salvianolic acids and preparing the high-content salvianolic acid B. According to the method, water phase is selectively removed via a method of organic solvent extraction without affecting on water-soluble components, and the rest of water phase is acidized and then extracted, so that the water-soluble components with high polarity such as inorganic salt, tannin, sugar and protein and can be selectively removed; finally, the high-content total salvianolic acid is obtained. Based on the obtained total salvianolic acid, salvianolic acid B product having content being more than 95% is finally obtained with the final yield about 3% by carrying out the steps of regulating pH of the total salvianolic acid solution within 6.0 to 8.0, separating the total salvianolic acid solution via macroporous resin and the like. The method is simple to develop, effective and low in cost, and has an industrial value.

The invention relates to new use of B Salvianolic acid. The B Salvianolic acid has a strong inhibition effect to tyrosinase, thereby being used for whitening skin, removing freckle, resisting wrinkle, and treating hyperpigmentation diseases, and being applicable to food additives. The inhibition of the B Salvianolic acid to the activity of the tyrosinase is detected, the B Salvianolic acid is very high in the activity of the tyrosinase, and is better in stability, and the B Salvianolic acid can effectively inhibit the generation of the melanin, and can be taken as the natural skin-whitening agent for cosmetics. Meanwhile, the B Salvianolic acid can more preferably promote the skin cell proliferation function, and has the functions of beautifying and resisting wrinkle, thereby being applicable to the cosmetics with the functions of beautifying and resisting wrinkle.

The invention belongs to the medicine technical field, in particular to a gastric retention preparation containing total salvianolic acid. The invention adopts the effective position of the Chinese medicine of total salvianolic acid as the main component, and the stomach retention preparation is made with acceptable auxiliary materials in pharmacy. The preparation of the invention is gastric floating preparation or stomach mucoadhesive preparation; the invention can significantly prolong the retention time of the total salvianolic acid on the stomach and the upper part of small intestine, enhance stability of medicine, and prolong effective absorption time and improve the utilizing degree of salvianolic acid biology through the double effect of enhancing the medicine stability and prolonging the effective absorption time. The invention can not only improve gastrointestinal absorption, but also have better efficacy in treating gastric ulcer and some other diseases; the invention also has simple production technique and is easy for industrialization; the invention is easy for the patients to take with good compliance.

The invention provides a medicinal composition containing general ginsenoside and total salvianolic acid and a preparation method thereof. The preparation method comprises the following steps of: performing heating reflux on ginseng in 60 percent (volume/volume) of aqueous solution of ethanol, after recovering the ethanol, allowing extract to pass through macroporous adsorbent resin, eluting by using water, 20 percent (volume/volume) aqueous solution of ethanol and 60 percent (volume/volume) aqueous solution of ethanol respectively, collecting a part which is eluted by 60 percent (volume/volume) aqueous solution of ethanol, and removing solvent to obtain general ginsenoside extract; leaching root of red-rooted salvia in water by heating, adding the ethanol into the extract until the volume of the ethanol is 80 percent of that of the extract, standing, filtering, after recovering the ethanol, allowing filter liquor to pass through the macroporous adsorbent resin, eluting by using the water and 40 percent (volume/volume) of aqueous solution of ethanol, collecting a part which is eluted by 40 percent (volume/volume) of aqueous solution of ethanol, and removing the solvent to obtain total salvianolic acid extract; and mixing the general ginsenoside extract and the total salvianolic acid extract according to a weight ratio of (0.1-10):(0.1-10).

The invention discloses a method for extracting salvianolic acid A, which is characterized in that a new process method is adopted to obtain salvianolic acid A extract, the yield of the salvianolic acid A in the salvianolic acid A extract prepared with the method is more than five thousandth, thus saving a great quantity of resources. The invention further discloses a method for preparing the salvianolic acid A, which comprises the steps of adopting a medium pressure liquid phase preparation method, wherein eluent has two phases to carry out isocratic or gradient elution to obtain high-purity salvianolic acid A with related substances, such as salvianolic acid F and salvianolic acid C, with content lower than 0.5 percent. The quality of the salvianolic acid A obtained by using the method is more uniform and more stable.

The invention provides a water-soluble salvia miltiorrhiza extractive, wherein the weight percent of total phenolic acid is 80-100 percent. The water-soluble salvia miltiorrhiza extractive is characterized by comprising the following components in percent by weight: 40-90 percent of salvianolic acid B, 3.0-15 percent of rosmarinic acid, 2-10 percent of alkannic acid and 0.2-2.2 percent of salvianolic acid E. The invention also provides a preparation containing the extractive and the extractive.

The invention relates to application of polymer salvianolic acid in preparation of drugs used for inhibiting occurrence or development of aortic aneurysm or aortic dissection and a pharmaceutical composition comprising the polymer salvianolic acid used as a pharmaceutical active substance, related desired adjuvant and the like. The pharmaceutical composition can be used for inhibiting occurrence of aortic aneurysm or aortic dissection. The polymer salvianolic acid is dimer salvianolic acid, trimer salvianolic acid or tetramer salvianolic acid, wherein the dimer salvianolic acid is rosmarinic acid, the trimer salvianolic acid is salvianolic acid C, alkannic acid or salvianolic acid A, and the tetramer salvianolic acid is salvianolic acid B.